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Article: Upregulation of chondroitin 6-sulphotransferase-1 facilities Schwann cell migration during axonal growth

TitleUpregulation of chondroitin 6-sulphotransferase-1 facilities Schwann cell migration during axonal growth
Authors
KeywordsChondroitin sulphate proteoglycans
Development
Dorsal root ganglion
Nerve regeneration
Sciatic nerve crush
Issue Date2006
PublisherCompany of Biologists Ltd.
Citation
Journal Of Cell Science, 2006, v. 119 n. 5, p. 933-942 How to Cite?
AbstractCell migration is central to development and posttraumatic regeneration. The differential increase in 6-sulphated chondroitins during axonal growth in both crushed sciatic nerves and brain development suggests that chondroitin 6-sulphotransferase-1 (C6ST-1) is a key enzyme that mediates cell migration in the process. We have cloned the cDNA of the C6ST-1 gene (C6st1) (GenBank accession number AF178689) from crushed sciatic nerves of adult rats and produced ribonucleotide probes accordingly to track signs of 6-sulphated chondroitins at the site of injury. We found C6st1 mRNA expression in Schwann cells emigrating from explants of both sciatic nerve segments and embryonic dorsal root ganglia. Immunocytochemistry indicated pericellular 6-sulphated chondroitin products around C6ST-1-expressing frontier cells. Motility analysis of frontier cells in cultures subjected to staged treatment with chondroitinase ABC indicated that freshly produced 6-sulphated chondroitin moieties facilitated Schwann cell motility, unlike restrictions resulting from proteoglycan interaction with matrix components. Sciatic nerve crush provided further evidence of in vivo upregulation of the C6ST-1 gene in mobile Schwann cells that guided axonal regrowth 1-14 days post crush; downregulation then accompanied declining mobility of Schwann cells as they engaged in the myelination of re-growing axons. These findings are the first to identify upregulated C6st1 gene expression correlating with the motility of Schwann cells that guide growing axons through both developmental and injured environments.
Persistent Identifierhttp://hdl.handle.net/10722/44575
ISSN
2015 Impact Factor: 4.706
2015 SCImago Journal Rankings: 3.501
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Jen_HK
dc.contributor.authorChau, CHen_HK
dc.contributor.authorLiu, Hen_HK
dc.contributor.authorJang, BRen_HK
dc.contributor.authorLi, Xen_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorShum, DKYen_HK
dc.date.accessioned2007-10-30T06:04:45Z-
dc.date.available2007-10-30T06:04:45Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal Of Cell Science, 2006, v. 119 n. 5, p. 933-942en_HK
dc.identifier.issn0021-9533en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44575-
dc.description.abstractCell migration is central to development and posttraumatic regeneration. The differential increase in 6-sulphated chondroitins during axonal growth in both crushed sciatic nerves and brain development suggests that chondroitin 6-sulphotransferase-1 (C6ST-1) is a key enzyme that mediates cell migration in the process. We have cloned the cDNA of the C6ST-1 gene (C6st1) (GenBank accession number AF178689) from crushed sciatic nerves of adult rats and produced ribonucleotide probes accordingly to track signs of 6-sulphated chondroitins at the site of injury. We found C6st1 mRNA expression in Schwann cells emigrating from explants of both sciatic nerve segments and embryonic dorsal root ganglia. Immunocytochemistry indicated pericellular 6-sulphated chondroitin products around C6ST-1-expressing frontier cells. Motility analysis of frontier cells in cultures subjected to staged treatment with chondroitinase ABC indicated that freshly produced 6-sulphated chondroitin moieties facilitated Schwann cell motility, unlike restrictions resulting from proteoglycan interaction with matrix components. Sciatic nerve crush provided further evidence of in vivo upregulation of the C6ST-1 gene in mobile Schwann cells that guided axonal regrowth 1-14 days post crush; downregulation then accompanied declining mobility of Schwann cells as they engaged in the myelination of re-growing axons. These findings are the first to identify upregulated C6st1 gene expression correlating with the motility of Schwann cells that guide growing axons through both developmental and injured environments.en_HK
dc.format.extent3321420 bytes-
dc.format.extent1803 bytes-
dc.format.extent1980 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherCompany of Biologists Ltd.en_HK
dc.relation.ispartofJournal of Cell Scienceen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectChondroitin sulphate proteoglycansen_HK
dc.subjectDevelopmenten_HK
dc.subjectDorsal root ganglionen_HK
dc.subjectNerve regenerationen_HK
dc.subjectSciatic nerve crushen_HK
dc.subject.meshAxons-physiologyen_HK
dc.subject.meshSchwann-Cells-metabolismen_HK
dc.subject.meshSulfotransferases-metabolismen_HK
dc.titleUpregulation of chondroitin 6-sulphotransferase-1 facilities Schwann cell migration during axonal growthen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9533&volume=119&issue=Pt 5&spage=933&epage=942&date=2006&atitle=Upregulation+of+chondroitin+6-sulphotransferase-1+facilitates+Schwann+cell+migration+during+axonal+growthen_HK
dc.identifier.emailChau, CH: mchchau@hkucc.hku.hken_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityChau, CH=rp00398en_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1242/jcs.02796en_HK
dc.identifier.pmid16495484en_HK
dc.identifier.scopuseid_2-s2.0-33645230959en_HK
dc.identifier.hkuros121191-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33645230959&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume119en_HK
dc.identifier.issue5en_HK
dc.identifier.spage933en_HK
dc.identifier.epage942en_HK
dc.identifier.isiWOS:000236385300015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLiu, J=36071959600en_HK
dc.identifier.scopusauthoridChau, CH=10040110500en_HK
dc.identifier.scopusauthoridLiu, H=12792847900en_HK
dc.identifier.scopusauthoridJang, BR=12793193100en_HK
dc.identifier.scopusauthoridLi, X=8093673500en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK
dc.identifier.scopusauthoridShum, DKY=7004824447en_HK

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