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Article: DNA fingerprinting elicited evolutionary trend of oral Candida tropicalis isolates from diverse geographic locales

TitleDNA fingerprinting elicited evolutionary trend of oral Candida tropicalis isolates from diverse geographic locales
Authors
KeywordsCandida tropicalis
Evolution
Fingerprinting
PCR
Issue Date2006
PublisherMedknow Publications Pvt Ltd. The Journal's web site is located at http://www.ijmm.org
Citation
Indian Journal of Medical Microbiology, 2006, v. 24 n. 3, p. 186-194 How to Cite?
AbstractPurpose: To study molecular profiles of oral Candida tropicalis isolates from five different geographic locales to determine the molecular diversity, clonality and evolutionary trends of this opportunistic pathogen. Methods: A total of 36 strains from five countries (China, Canada, Scotland, Japan and Tanzania) were genotyped by PCR fingerprinting with 11 separate primers. Of these, primers RSG9, RSG8, T3B and RSD12 generated complex fingerprinting patterns. Results: Three significantly dissimilar profiles were derived from the primer T3B and particularly focused on tDNA suggested the prevalence of genetic subtypes within the species. Comparison of tDNA and rDNA (RSD12) fingerprints of C. tropicalis suggested that rDNA is much more heterogeneous than the relatively distinct tDNA. Further analysis of similarity coefficient (S AB) of gel profiles derived from computer-generated dendrograms indicated some degree of similarity in isolates from five-disparate geographic locales as well as the presence of unique isotypes in each region. Conclusions: This study demonstrates the evolutionary divergence of distinct genetic subgroups within Candida tropicalis.
Persistent Identifierhttp://hdl.handle.net/10722/44545
ISSN
2015 Impact Factor: 1.006
2015 SCImago Journal Rankings: 0.530
References

 

DC FieldValueLanguage
dc.contributor.authorDassanayake, RSen_HK
dc.contributor.authorSamaranayake, Yen_HK
dc.contributor.authorYau, JJYen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2007-10-30T06:03:53Z-
dc.date.available2007-10-30T06:03:53Z-
dc.date.issued2006en_HK
dc.identifier.citationIndian Journal of Medical Microbiology, 2006, v. 24 n. 3, p. 186-194en_HK
dc.identifier.issn0255-0857en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44545-
dc.description.abstractPurpose: To study molecular profiles of oral Candida tropicalis isolates from five different geographic locales to determine the molecular diversity, clonality and evolutionary trends of this opportunistic pathogen. Methods: A total of 36 strains from five countries (China, Canada, Scotland, Japan and Tanzania) were genotyped by PCR fingerprinting with 11 separate primers. Of these, primers RSG9, RSG8, T3B and RSD12 generated complex fingerprinting patterns. Results: Three significantly dissimilar profiles were derived from the primer T3B and particularly focused on tDNA suggested the prevalence of genetic subtypes within the species. Comparison of tDNA and rDNA (RSD12) fingerprints of C. tropicalis suggested that rDNA is much more heterogeneous than the relatively distinct tDNA. Further analysis of similarity coefficient (S AB) of gel profiles derived from computer-generated dendrograms indicated some degree of similarity in isolates from five-disparate geographic locales as well as the presence of unique isotypes in each region. Conclusions: This study demonstrates the evolutionary divergence of distinct genetic subgroups within Candida tropicalis.en_HK
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dc.format.extent1842 bytes-
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dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherMedknow Publications Pvt Ltd. The Journal's web site is located at http://www.ijmm.orgen_HK
dc.relation.ispartofIndian Journal of Medical Microbiologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsIndian Journal of Medical Microbiology. Copyright © Medknow Publications Pvt Ltd.-
dc.subjectCandida tropicalisen_HK
dc.subjectEvolutionen_HK
dc.subjectFingerprintingen_HK
dc.subjectPCRen_HK
dc.subject.meshCandida-tropicalis-geneticsen_HK
dc.subject.meshCandidiasis,-Oral-epidemiologyen_HK
dc.subject.meshCandidiasis,-Oral-microbiologyen_HK
dc.subject.meshDNA-Fingerprintingen_HK
dc.titleDNA fingerprinting elicited evolutionary trend of oral Candida tropicalis isolates from diverse geographic localesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0255-0857&volume=24&issue=3&spage=186&epage=194&date=2006&atitle=DNA+fingerprinting+elicited+evolutionary+trend+of+oral+Candida+tropicalis+isolates+from+diverse+geographic+localesen_HK
dc.identifier.emailSamaranayake, YH: hema@hkucc.hku.hken_HK
dc.identifier.emailYau, JYY: yaujyy@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP: lakshman@hku.hk-
dc.identifier.authoritySamaranayake, Y=rp00025en_HK
dc.identifier.authoritySamaranayake, L=rp00023en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid16912438-
dc.identifier.scopuseid_2-s2.0-33748150830en_HK
dc.identifier.hkuros119285-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33748150830&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue3en_HK
dc.identifier.spage186en_HK
dc.identifier.epage194en_HK
dc.publisher.placeIndiaen_HK
dc.identifier.scopusauthoridDassanayake, R=6603321318en_HK
dc.identifier.scopusauthoridSamaranayake, Y=6602677237en_HK
dc.identifier.scopusauthoridYau, JJY=14326402900en_HK
dc.identifier.scopusauthoridSamaranayake, L=7102761002en_HK

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