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Article: Characterization of the segmental duplication LCR7-20 in the human genome

TitleCharacterization of the segmental duplication LCR7-20 in the human genome
Authors
Issue Date2004
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygeno
Citation
Genomics, 2004, v. 83 n. 2, p. 262-269 How to Cite?
AbstractOur previous study described the amplification of a genomic sequence containing exon 9 of CFTR in the human genome. Here we report that this CFTR sequence is part of a large duplicated sequence unit, provisionally named LCR7-20. Through successive screening of two human chromosome 7-specific cosmid libraries to construct a cosmid contig, we assembled two sequenced BAC clones into a single contig containing a prototypic LCR7-20 unit. Subsequent searches of existing human genome sequences identified additional six copies of LCR7-20-like sequences with more than 90% sequence homology. Additional genomic clones containing LCR7-20-like sequences were then isolated from total genomic BAC and PAC libraries. Restriction fragment analysis and limited sequencing data indicated that there could be around 30 copies of LCR7-20-like sequences in the human genome and that the average region of homology could extend over 120 kb. As indicated by fluorescence in situ hybridization analysis, LCR7-20-like sequences are dispersed on different chromosomes, mainly in the centromeric and pericentromeric regions, and some may exist in tandem copies. Our study also indicates that many genomic regions containing LCR7-20's either have been misassembled or are missing in current versions of the human genome sequence. © 2003 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/44386
ISSN
2015 Impact Factor: 2.386
2015 SCImago Journal Rankings: 1.281
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Xen_HK
dc.contributor.authorLi, Xen_HK
dc.contributor.authorLi, Men_HK
dc.contributor.authorAcimovic, YJen_HK
dc.contributor.authorLi, Zen_HK
dc.contributor.authorScherer, SWen_HK
dc.contributor.authorEstivill, Xen_HK
dc.contributor.authorTsui, LCen_HK
dc.date.accessioned2007-09-12T03:52:32Z-
dc.date.available2007-09-12T03:52:32Z-
dc.date.issued2004en_HK
dc.identifier.citationGenomics, 2004, v. 83 n. 2, p. 262-269en_HK
dc.identifier.issn0888-7543en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44386-
dc.description.abstractOur previous study described the amplification of a genomic sequence containing exon 9 of CFTR in the human genome. Here we report that this CFTR sequence is part of a large duplicated sequence unit, provisionally named LCR7-20. Through successive screening of two human chromosome 7-specific cosmid libraries to construct a cosmid contig, we assembled two sequenced BAC clones into a single contig containing a prototypic LCR7-20 unit. Subsequent searches of existing human genome sequences identified additional six copies of LCR7-20-like sequences with more than 90% sequence homology. Additional genomic clones containing LCR7-20-like sequences were then isolated from total genomic BAC and PAC libraries. Restriction fragment analysis and limited sequencing data indicated that there could be around 30 copies of LCR7-20-like sequences in the human genome and that the average region of homology could extend over 120 kb. As indicated by fluorescence in situ hybridization analysis, LCR7-20-like sequences are dispersed on different chromosomes, mainly in the centromeric and pericentromeric regions, and some may exist in tandem copies. Our study also indicates that many genomic regions containing LCR7-20's either have been misassembled or are missing in current versions of the human genome sequence. © 2003 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygenoen_HK
dc.relation.ispartofGenomicsen_HK
dc.subject.meshChromosomes, human, pair 7en_HK
dc.subject.meshContig mappingen_HK
dc.subject.meshGene duplicationen_HK
dc.subject.meshGenome, humanen_HK
dc.subject.meshCystic fibrosis transmembrane conductance regulator - geneticsen_HK
dc.titleCharacterization of the segmental duplication LCR7-20 in the human genomeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0888-7543&volume=83&issue=2&spage=262&epage=269&date=2004&atitle=Characterization+of+the+segmental+duplication+LCR7-20+in+the+human+genomeen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1016/j.ygeno.2003.08.003en_HK
dc.identifier.pmid14706455-
dc.identifier.scopuseid_2-s2.0-0346728739en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0346728739&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume83en_HK
dc.identifier.issue2en_HK
dc.identifier.spage262en_HK
dc.identifier.epage269en_HK
dc.identifier.isiWOS:000188211400008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLiu, X=36076945600en_HK
dc.identifier.scopusauthoridLi, X=37109238900en_HK
dc.identifier.scopusauthoridLi, M=7405264026en_HK
dc.identifier.scopusauthoridAcimovic, YJ=6506724119en_HK
dc.identifier.scopusauthoridLi, Z=22135340200en_HK
dc.identifier.scopusauthoridScherer, SW=35374654500en_HK
dc.identifier.scopusauthoridEstivill, X=36047834200en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK

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