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Article: Murine phosphatidylserine-specific phospholipase A1 (Ps-pla1) maps to Chromosome 16 but is distinct from the lpd (lipid defect) locus

TitleMurine phosphatidylserine-specific phospholipase A1 (Ps-pla1) maps to Chromosome 16 but is distinct from the lpd (lipid defect) locus
Authors
Issue Date2001
PublisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00335/
Citation
Mammalian Genome, 2001, v. 12 n. 2, p. 129-132 How to Cite?
AbstractWe have previously generated a mouse transgenic line with an insertional mutation designated lpd that demonstrates a phenotype of hypertriglyceridemia and fatty liver. Since the recently identified phosphatidylserine-specific phospholipase A1 (PS-PLA1) demonstrates significant homology to triglyceride lipases, we reasoned that the mouse Ps-pla1 gene may be the disrupted gene within the lpd locus. Using a rat PS-PLA1 cDNA sequence to search the EST database, we identified a mouse EST homolog AA839424. Sequencing analysis of AA839424 revealed a putative Ps-pla1 protein of 456 amino acids with extensive overall structural conservation with human and rat PS-PLA1 and with triglyceride lipases. Conserved sequences in Ps-pla1 include a lipase consensus sequences G×S×G, a catalytic triad, and eight of the ten conserved cysteine residues that are required for tertiary structure. Mouse Ps-pla1 carries a phosphatidylserine-binding motif that is absent in all triglyceride lipases. Using a mouse whole-genome radiation hybrid (WG-RH) mapping panel (T31), we mapped mouse Ps-pla1 to Chromosome (Chr) 16 between genetic markers D16Mit194 and D16Mit38, which is 17.1 cM centromeric to the lpd locus. On the basis of chromosome location, we conclude that Ps-pla1 and lpd are distinct genes in lipid metabolism.
Persistent Identifierhttp://hdl.handle.net/10722/44364
ISSN
2015 Impact Factor: 2.078
2015 SCImago Journal Rankings: 2.082
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWen, XYen_HK
dc.contributor.authorStewart, AKen_HK
dc.contributor.authorSkaug, Jen_HK
dc.contributor.authorWei, Een_HK
dc.contributor.authorTsui, LCen_HK
dc.date.accessioned2007-09-12T03:52:09Z-
dc.date.available2007-09-12T03:52:09Z-
dc.date.issued2001en_HK
dc.identifier.citationMammalian Genome, 2001, v. 12 n. 2, p. 129-132en_HK
dc.identifier.issn0938-8990en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44364-
dc.description.abstractWe have previously generated a mouse transgenic line with an insertional mutation designated lpd that demonstrates a phenotype of hypertriglyceridemia and fatty liver. Since the recently identified phosphatidylserine-specific phospholipase A1 (PS-PLA1) demonstrates significant homology to triglyceride lipases, we reasoned that the mouse Ps-pla1 gene may be the disrupted gene within the lpd locus. Using a rat PS-PLA1 cDNA sequence to search the EST database, we identified a mouse EST homolog AA839424. Sequencing analysis of AA839424 revealed a putative Ps-pla1 protein of 456 amino acids with extensive overall structural conservation with human and rat PS-PLA1 and with triglyceride lipases. Conserved sequences in Ps-pla1 include a lipase consensus sequences G×S×G, a catalytic triad, and eight of the ten conserved cysteine residues that are required for tertiary structure. Mouse Ps-pla1 carries a phosphatidylserine-binding motif that is absent in all triglyceride lipases. Using a mouse whole-genome radiation hybrid (WG-RH) mapping panel (T31), we mapped mouse Ps-pla1 to Chromosome (Chr) 16 between genetic markers D16Mit194 and D16Mit38, which is 17.1 cM centromeric to the lpd locus. On the basis of chromosome location, we conclude that Ps-pla1 and lpd are distinct genes in lipid metabolism.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00335/en_HK
dc.relation.ispartofMammalian Genomeen_HK
dc.rightsThe original publication is available at www.springerlink.comen_HK
dc.subject.meshPhosphatidylserines - metabolismen_HK
dc.subject.meshPhospholipases a - geneticsen_HK
dc.subject.meshRadiation hybrid mappingen_HK
dc.subject.meshSequence analysis, dnaen_HK
dc.subject.meshExpressed sequence tagsen_HK
dc.titleMurine phosphatidylserine-specific phospholipase A1 (Ps-pla1) maps to Chromosome 16 but is distinct from the lpd (lipid defect) locusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0938-8990&volume=12&issue=2&spage=129&epage=132&date=2001&atitle=Murine+phosphatidylserine-specific+phospholipase+A1+(Ps-pla1)+maps+to+chromosome+16+but+is+distinct+from+the+lpd+(lipid+defect)+locusen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1007/s003350010256en_HK
dc.identifier.pmid11210182-
dc.identifier.scopuseid_2-s2.0-0035121574en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035121574&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume12en_HK
dc.identifier.issue2en_HK
dc.identifier.spage129en_HK
dc.identifier.epage132en_HK
dc.identifier.isiWOS:000166684200007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWen, XY=55197619700en_HK
dc.identifier.scopusauthoridStewart, AK=7403496983en_HK
dc.identifier.scopusauthoridSkaug, J=6603258009en_HK
dc.identifier.scopusauthoridWei, E=8044586400en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK

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