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Article: Physical mapping of a tandem duplication on the long arm of chromosome 7 associated with a multidrug resistant phenotype

TitlePhysical mapping of a tandem duplication on the long arm of chromosome 7 associated with a multidrug resistant phenotype
Authors
Issue Date1999
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cancergene
Citation
Cancer Genetics And Cytogenetics, 1999, v. 110 n. 1, p. 28-33 How to Cite?
AbstractBoth the expression of the multidrug transporter, P-glycoprotein (Pgp), and abnormalities of the long arm of chromosome 7 have been shown to be adverse prognostic indicators in acute leukemias. In this study, a clonal duplication, dup(7)(q11.1q31.1), inherited with the classical multidrug resistant phenotype in a drug-resistant derivative of a human T-cell leukemia cell line was characterized. The position of the duplication was of interest as the gene which encodes Pgp, MDR1, is located on the long arm of chromosome 7 at position 7q21.1. Fluorescence in situ hybridization (FISH) analysis with a chromosome 7-specific painting probe confirmed the composition of the abnormal chromosome. A YAC clone hybridizing to the MDR1 locus confirmed that this gene was located within the duplicated region of the derivative chromosome. With a panel of well-characterized YAC clones, the duplicated segment was found to be a direct tandem duplication, somewhat larger than estimated by conventional cytogenetics. The proximal and distal breakpoints of the abnormality were located and a YAC clone spanning the distal breakpoint was identified. This clone is of particular interest, as it harbors the markers D7S523 and D7S471, close to which a putative tumor suppressor gene is thought to lie. Further examination of the breakpoint region may therefore illuminate the mechanism of Pgp upregulation as well as providing information about a tumor suppressor gene.
Persistent Identifierhttp://hdl.handle.net/10722/44351
ISSN
2012 Impact Factor: 1.929
2013 SCImago Journal Rankings: 0.872
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDe Silva, MGen_HK
dc.contributor.authorKantharidis, Pen_HK
dc.contributor.authorScherer, SWen_HK
dc.contributor.authorRayeroux, Ken_HK
dc.contributor.authorCampbell, Len_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorZalcberg, JRen_HK
dc.date.accessioned2007-09-12T03:51:55Z-
dc.date.available2007-09-12T03:51:55Z-
dc.date.issued1999en_HK
dc.identifier.citationCancer Genetics And Cytogenetics, 1999, v. 110 n. 1, p. 28-33en_HK
dc.identifier.issn0165-4608en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44351-
dc.description.abstractBoth the expression of the multidrug transporter, P-glycoprotein (Pgp), and abnormalities of the long arm of chromosome 7 have been shown to be adverse prognostic indicators in acute leukemias. In this study, a clonal duplication, dup(7)(q11.1q31.1), inherited with the classical multidrug resistant phenotype in a drug-resistant derivative of a human T-cell leukemia cell line was characterized. The position of the duplication was of interest as the gene which encodes Pgp, MDR1, is located on the long arm of chromosome 7 at position 7q21.1. Fluorescence in situ hybridization (FISH) analysis with a chromosome 7-specific painting probe confirmed the composition of the abnormal chromosome. A YAC clone hybridizing to the MDR1 locus confirmed that this gene was located within the duplicated region of the derivative chromosome. With a panel of well-characterized YAC clones, the duplicated segment was found to be a direct tandem duplication, somewhat larger than estimated by conventional cytogenetics. The proximal and distal breakpoints of the abnormality were located and a YAC clone spanning the distal breakpoint was identified. This clone is of particular interest, as it harbors the markers D7S523 and D7S471, close to which a putative tumor suppressor gene is thought to lie. Further examination of the breakpoint region may therefore illuminate the mechanism of Pgp upregulation as well as providing information about a tumor suppressor gene.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cancergeneen_HK
dc.relation.ispartofCancer Genetics and Cytogeneticsen_HK
dc.subject.meshChromosome aberrationsen_HK
dc.subject.meshChromosomes, human, pair 7en_HK
dc.subject.meshDrug resistance, multiple - geneticsen_HK
dc.subject.meshLeukemia, t-cell - drug therapy - geneticsen_HK
dc.subject.meshPhysical chromosome mappingen_HK
dc.titlePhysical mapping of a tandem duplication on the long arm of chromosome 7 associated with a multidrug resistant phenotypeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0165-4608&volume=110&issue=1&spage=28&epage=33&date=1999&atitle=Physical+mapping+of+a+tandem+duplication+on+the+long+arm+of+chromosome+7+associated+with+a+multidrug+resistant+phenotypeen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1016/S0165-4608(98)00195-2en_HK
dc.identifier.pmid10198619-
dc.identifier.scopuseid_2-s2.0-0032922156en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032922156&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume110en_HK
dc.identifier.issue1en_HK
dc.identifier.spage28en_HK
dc.identifier.epage33en_HK
dc.identifier.isiWOS:000079485600006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDe Silva, MG=7103414142en_HK
dc.identifier.scopusauthoridKantharidis, P=6602818315en_HK
dc.identifier.scopusauthoridScherer, SW=35374654500en_HK
dc.identifier.scopusauthoridRayeroux, K=6505841075en_HK
dc.identifier.scopusauthoridCampbell, L=7403165735en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridZalcberg, JR=7005883541en_HK

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