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Article: Localization of 67 exons on a YAC contig spanning 1.5 Mb around the multidrug resistance gene region of human chromosome 7q21.1

TitleLocalization of 67 exons on a YAC contig spanning 1.5 Mb around the multidrug resistance gene region of human chromosome 7q21.1
Authors
Issue Date1998
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygeno
Citation
Genomics, 1998, v. 49 n. 1, p. 14-22 How to Cite?
AbstractA contig of 21 nonchimeric yeast artificial chromosomes (YACs) was previously assembled across 1.5 Mb of the multidrug resistance (MDR) gene (PGY1 and PGY3) region of human chromosome 7q21.1. This region of the human genome has now been subjected to exon amplification to detect the presence of additional genes. Exon trapping was performed directly on the YACs. Sixty- seven gene fragments were isolated and characterized by sequence analysis and comparison with public databases. The localization of these exons in the 1.5- Mb region was determined by hybridization to YAC clones, and they were localized in 11 subregions of YAC contigs. The exon collection includes 21 exons that were identical to known cDNA sequences of PGY1, PGY3, sorcin (SRI), the cDNA similar to the δ subunit of the human amiloride-sensitive Na+ channel (SCNED), and 4 cDNAs with unknown function; 43 exons that showed homology/similarity to known cDNA sequences of mouse DMP1, rat COT, mouse and human NADHD, human MDC, 3 cDNAs encoding possible membrane proteins, and 21 other cDNAs; and 3 exons that shared no homology/similarity with any sequence in public databases. The nucleotide sequences of all the PGY1 and PGY3 exons were identical to the corresponding cDNA sequences previously determined, and these exons were localized to the expected positions on the appropriate YAC clones. No other member of the MDR gene family thus appeared to be present in the 1.5-Mb region. The integrated physical and exon maps should prove valuable for both fine mapping and determination of a complete gene map of this segment of the genome.
Persistent Identifierhttp://hdl.handle.net/10722/44340
ISSN
2015 Impact Factor: 2.386
2015 SCImago Journal Rankings: 1.281
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTorigoe, Ken_HK
dc.contributor.authorHarada, Ten_HK
dc.contributor.authorKusaba, Hen_HK
dc.contributor.authorUchiumi, Ten_HK
dc.contributor.authorKohno, Ken_HK
dc.contributor.authorGreen, EDen_HK
dc.contributor.authorScherer, SWen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorSchlessinger, Den_HK
dc.contributor.authorKuwano, Men_HK
dc.contributor.authorWada, Men_HK
dc.date.accessioned2007-09-12T03:51:43Z-
dc.date.available2007-09-12T03:51:43Z-
dc.date.issued1998en_HK
dc.identifier.citationGenomics, 1998, v. 49 n. 1, p. 14-22en_HK
dc.identifier.issn0888-7543en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44340-
dc.description.abstractA contig of 21 nonchimeric yeast artificial chromosomes (YACs) was previously assembled across 1.5 Mb of the multidrug resistance (MDR) gene (PGY1 and PGY3) region of human chromosome 7q21.1. This region of the human genome has now been subjected to exon amplification to detect the presence of additional genes. Exon trapping was performed directly on the YACs. Sixty- seven gene fragments were isolated and characterized by sequence analysis and comparison with public databases. The localization of these exons in the 1.5- Mb region was determined by hybridization to YAC clones, and they were localized in 11 subregions of YAC contigs. The exon collection includes 21 exons that were identical to known cDNA sequences of PGY1, PGY3, sorcin (SRI), the cDNA similar to the δ subunit of the human amiloride-sensitive Na+ channel (SCNED), and 4 cDNAs with unknown function; 43 exons that showed homology/similarity to known cDNA sequences of mouse DMP1, rat COT, mouse and human NADHD, human MDC, 3 cDNAs encoding possible membrane proteins, and 21 other cDNAs; and 3 exons that shared no homology/similarity with any sequence in public databases. The nucleotide sequences of all the PGY1 and PGY3 exons were identical to the corresponding cDNA sequences previously determined, and these exons were localized to the expected positions on the appropriate YAC clones. No other member of the MDR gene family thus appeared to be present in the 1.5-Mb region. The integrated physical and exon maps should prove valuable for both fine mapping and determination of a complete gene map of this segment of the genome.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygenoen_HK
dc.relation.ispartofGenomicsen_HK
dc.subject.meshChromosomes, human, pair 7en_HK
dc.subject.meshExonsen_HK
dc.subject.meshP-glycoproteinsen_HK
dc.subject.meshGenes, mdren_HK
dc.subject.meshNadh dehydrogenase - geneticsen_HK
dc.titleLocalization of 67 exons on a YAC contig spanning 1.5 Mb around the multidrug resistance gene region of human chromosome 7q21.1en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0888-7543&volume=49&issue=1&spage=14&epage=22&date=1998&atitle=Localization+of+67+exons+on+a+YAC+contig+spanning+1.5+Mb+around+the+multidrug+resistance+gene+region+of+human+chromosome+7q21.1en_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1006/geno.1997.5200en_HK
dc.identifier.pmid9570944-
dc.identifier.scopuseid_2-s2.0-0032054780en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032054780&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume49en_HK
dc.identifier.issue1en_HK
dc.identifier.spage14en_HK
dc.identifier.epage22en_HK
dc.identifier.isiWOS:000073166900002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTorigoe, K=7006588353en_HK
dc.identifier.scopusauthoridHarada, T=35467447700en_HK
dc.identifier.scopusauthoridKusaba, H=6701575870en_HK
dc.identifier.scopusauthoridUchiumi, T=7006335911en_HK
dc.identifier.scopusauthoridKohno, K=7201467409en_HK
dc.identifier.scopusauthoridGreen, ED=7201576916en_HK
dc.identifier.scopusauthoridScherer, SW=35374654500en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridSchlessinger, D=7103215816en_HK
dc.identifier.scopusauthoridKuwano, M=7103200772en_HK
dc.identifier.scopusauthoridWada, M=7401843807en_HK

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