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Article: Molecular characterization of a common fragile site (FRA7H) on human chromosome 7 by the cloning of a simian virus 40 integration site

TitleMolecular characterization of a common fragile site (FRA7H) on human chromosome 7 by the cloning of a simian virus 40 integration site
Authors
Issue Date1998
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings Of The National Academy Of Sciences Of The United States Of America, 1998, v. 95 n. 14, p. 8141-8146 How to Cite?
AbstractCommon fragile sites are chromosomal loci prone to breakage and rearrangement, hypothesized to provide targets for foreign DNA integration. We cloned a simian virus 40 integration site and showed by fluorescent in situ hybridization analysis that the integration event had occurred within a common aphidicolin-induced fragile site on human chromosome 7, FRA7H. A region of 161 kb spanning FRA7H was defined and sequenced. Several regions with a potential unusual DNA structure, including high-flexibility, low- stability, and non-B-DNA-forming sequences were identified in this region. We performed a similar analysis on the published FRA3B sequence and the putative partial FRA7G, which also revealed an impressive cluster of regions with high flexibility and low stability. Thus, these unusual DNA characteristics are possibly intrinsic properties of common fragile sites that may affect their replication and condensation as well as organization, and may lead to fragility.
Persistent Identifierhttp://hdl.handle.net/10722/44338
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMishmar, Den_HK
dc.contributor.authorRahat, Aen_HK
dc.contributor.authorScherer, SWen_HK
dc.contributor.authorNyakatura, Gen_HK
dc.contributor.authorHinzmann, Ben_HK
dc.contributor.authorKohwi, Yen_HK
dc.contributor.authorMandelGutfroind, Yen_HK
dc.contributor.authorLee, JRen_HK
dc.contributor.authorDrescher, Ben_HK
dc.contributor.authorSas, DEen_HK
dc.contributor.authorMargalit, Hen_HK
dc.contributor.authorPlatzer, Men_HK
dc.contributor.authorWeiss, Aen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorRosenthal, Aen_HK
dc.contributor.authorKerem, Ben_HK
dc.date.accessioned2007-09-12T03:51:41Z-
dc.date.available2007-09-12T03:51:41Z-
dc.date.issued1998en_HK
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America, 1998, v. 95 n. 14, p. 8141-8146en_HK
dc.identifier.issn0027-8424en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44338-
dc.description.abstractCommon fragile sites are chromosomal loci prone to breakage and rearrangement, hypothesized to provide targets for foreign DNA integration. We cloned a simian virus 40 integration site and showed by fluorescent in situ hybridization analysis that the integration event had occurred within a common aphidicolin-induced fragile site on human chromosome 7, FRA7H. A region of 161 kb spanning FRA7H was defined and sequenced. Several regions with a potential unusual DNA structure, including high-flexibility, low- stability, and non-B-DNA-forming sequences were identified in this region. We performed a similar analysis on the published FRA3B sequence and the putative partial FRA7G, which also revealed an impressive cluster of regions with high flexibility and low stability. Thus, these unusual DNA characteristics are possibly intrinsic properties of common fragile sites that may affect their replication and condensation as well as organization, and may lead to fragility.en_HK
dc.languageengen_HK
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_HK
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_HK
dc.subject.meshChromosome fragilityen_HK
dc.subject.meshChromosomes, human, pair 7en_HK
dc.subject.meshDna transposable elements - geneticsen_HK
dc.subject.meshSimian virus 40 - geneticsen_HK
dc.subject.meshChromosome fragile sitesen_HK
dc.titleMolecular characterization of a common fragile site (FRA7H) on human chromosome 7 by the cloning of a simian virus 40 integration siteen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0027-8424&volume=95&issue=14&spage=8141&epage=8146&date=1998&atitle=Molecular+characterization+of+a+common+fragile+site+(FRA7H)+on+human+chromosome+7+by+the+cloning+of+a+simian+virus+40+integration+siteen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_OA_fulltexten_HK
dc.identifier.doi10.1073/pnas.95.14.8141en_HK
dc.identifier.pmid9653154-
dc.identifier.pmcidPMC20943-
dc.identifier.scopuseid_2-s2.0-13144283613en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-13144283613&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume95en_HK
dc.identifier.issue14en_HK
dc.identifier.spage8141en_HK
dc.identifier.epage8146en_HK
dc.identifier.isiWOS:000074717300055-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMishmar, D=7801384496en_HK
dc.identifier.scopusauthoridRahat, A=6602639524en_HK
dc.identifier.scopusauthoridScherer, SW=35374654500en_HK
dc.identifier.scopusauthoridNyakatura, G=6603291414en_HK
dc.identifier.scopusauthoridHinzmann, B=6603476156en_HK
dc.identifier.scopusauthoridKohwi, Y=6603841981en_HK
dc.identifier.scopusauthoridMandelGutfroind, Y=6504220696en_HK
dc.identifier.scopusauthoridLee, JR=19435064100en_HK
dc.identifier.scopusauthoridDrescher, B=35958059900en_HK
dc.identifier.scopusauthoridSas, DE=8130744200en_HK
dc.identifier.scopusauthoridMargalit, H=7003661401en_HK
dc.identifier.scopusauthoridPlatzer, M=55412750700en_HK
dc.identifier.scopusauthoridWeiss, A=7402247966en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridRosenthal, A=7203080010en_HK
dc.identifier.scopusauthoridKerem, B=35376353800en_HK

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