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Article: Defect in the maintenance of the apical ectodermal ridge in the Dactylaplasia mouse

TitleDefect in the maintenance of the apical ectodermal ridge in the Dactylaplasia mouse
Authors
Issue Date1998
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbio
Citation
Developmental Biology, 1998, v. 201 n. 1, p. 78-89 How to Cite?
AbstractDuring vertebrate limb development the distal apex of the limb bud ectoderm is induced to form the apical ectodermal ridge (AER). The presence of the AER is required for the continued outgrowth of the limb bud. Classical embryological studies have led to the hypothesis that a secreted mesenchymal factor is required to maintain the AER. We have undertaken a detailed analysis of Dactylaplasia (Dac) mice, a semidominant mutant which displays missing central digits in the fore- and hindlimbs of heterozygous animals and monodactyly in homozygous animals. Our data show that Dac mice have a defect in the maintenance of the AER. At E10.5, the mutant AER is found to be morphologically normal. However, by E11.5 the central aspect of the AER degenerates leaving the anterior and posterior AER intact. In homozygous mice both the central and anterior AER degenerate, while the posterior extremity of the AER is unaffected. Analysis of BrdU incorporation reveals that degeneration of the AER is due to a lack of cell proliferation in the mutant AER. The loss of the AER leads to a reduction in cell proliferation in the subridge mesenchyme at E11.5. The data represent direct genetic evidence for the existence of an AER maintenance activity that is distinct from AER induction and differentiation. Moreover, the data suggest that the role of the AER maintenance factor is to promote cell proliferation in the ridge. Based on our findings, we propose a model for AER maintenance in the vertebrate limb.
Persistent Identifierhttp://hdl.handle.net/10722/44337
ISSN
2015 Impact Factor: 3.155
2015 SCImago Journal Rankings: 2.554
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCrackower, MAen_HK
dc.contributor.authorMotoyama, Jen_HK
dc.contributor.authorTsui, LCen_HK
dc.date.accessioned2007-09-12T03:51:39Z-
dc.date.available2007-09-12T03:51:39Z-
dc.date.issued1998en_HK
dc.identifier.citationDevelopmental Biology, 1998, v. 201 n. 1, p. 78-89en_HK
dc.identifier.issn0012-1606en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44337-
dc.description.abstractDuring vertebrate limb development the distal apex of the limb bud ectoderm is induced to form the apical ectodermal ridge (AER). The presence of the AER is required for the continued outgrowth of the limb bud. Classical embryological studies have led to the hypothesis that a secreted mesenchymal factor is required to maintain the AER. We have undertaken a detailed analysis of Dactylaplasia (Dac) mice, a semidominant mutant which displays missing central digits in the fore- and hindlimbs of heterozygous animals and monodactyly in homozygous animals. Our data show that Dac mice have a defect in the maintenance of the AER. At E10.5, the mutant AER is found to be morphologically normal. However, by E11.5 the central aspect of the AER degenerates leaving the anterior and posterior AER intact. In homozygous mice both the central and anterior AER degenerate, while the posterior extremity of the AER is unaffected. Analysis of BrdU incorporation reveals that degeneration of the AER is due to a lack of cell proliferation in the mutant AER. The loss of the AER leads to a reduction in cell proliferation in the subridge mesenchyme at E11.5. The data represent direct genetic evidence for the existence of an AER maintenance activity that is distinct from AER induction and differentiation. Moreover, the data suggest that the role of the AER maintenance factor is to promote cell proliferation in the ridge. Based on our findings, we propose a model for AER maintenance in the vertebrate limb.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbioen_HK
dc.relation.ispartofDevelopmental Biologyen_HK
dc.subject.meshEctoderm - pathology - physiologyen_HK
dc.subject.meshEmbryo - ultrastructureen_HK
dc.subject.meshLimb bud - abnormalities - ultrastructureen_HK
dc.subject.meshToes - abnormalities - embryologyen_HK
dc.subject.meshMicroscopy, electron, scanningen_HK
dc.titleDefect in the maintenance of the apical ectodermal ridge in the Dactylaplasia mouseen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0012-1606&volume=201&issue=1&spage=78&epage=89&date=1998&atitle=Defect+in+the+maintenance+of+the+apical+ectodermal+ridge+in+the+Dactylaplasia+mouseen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1006/dbio.1998.8938en_HK
dc.identifier.pmid9733575en_HK
dc.identifier.scopuseid_2-s2.0-0032168778en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032168778&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume201en_HK
dc.identifier.issue1en_HK
dc.identifier.spage78en_HK
dc.identifier.epage89en_HK
dc.identifier.isiWOS:000075918500007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCrackower, MA=6701530321en_HK
dc.identifier.scopusauthoridMotoyama, J=6701556287en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK

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