File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Comparison between intratracheal and intravenous administration of liposome-DNA complexes for cystic fibrosis lung gene therapy

TitleComparison between intratracheal and intravenous administration of liposome-DNA complexes for cystic fibrosis lung gene therapy
Authors
KeywordsCationic liposomes
Cystic fibrosis
Lung gene therapy
Issue Date1998
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/gt
Citation
Gene Therapy, 1998, v. 5 n. 2, p. 181-188 How to Cite?
AbstractIntratracheal (i.t.) and intravenous (i.v.) delivery of DNA-vector formulations are two strategies to obtain gene transfer to the lung. It is still uncertain, however, which of these two modes of delivery will be more effective in the treatment of cystic fibrosis and other lung diseases. In this study, we attempted to optimize formulations of the cationic liposome DODAC:DOPE (dioleoyldimethylammoniumchloride:dioleoylphosphatidylethanolamine) complexed to plasmids encoding chloramphenicol acetyltransferase for i.t. and i.v. injection into CD-1 mice and compared the two methods. Our results showed that both methods conferred reporter gene expression in the lung that was significantly higher relative to injection of plasmid DNA alone. Expression using either mode of administration was maximal 24 h after injection and declined to around 10% of day 1 levels 2 weeks after injection. For i.v. delivery of DODAC:DOPE-DNA complexes multilamellar vesicles were more effective than large unilamellar vesicles in all organs investigated. Recombinant DNA could be detected in the distal lung region following either route of administration. However, i.t. administration predominantly led to DNA deposition in epithelial cells lining the bronchioles, eg in clara cells, whereas i.v. administration resulted in DNA deposition in the alveolar region of the lung including type II alveolar epithelial cells.
Persistent Identifierhttp://hdl.handle.net/10722/44335
ISSN
2015 Impact Factor: 3.242
2015 SCImago Journal Rankings: 1.519
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGriesenbach, Uen_HK
dc.contributor.authorChonn, Aen_HK
dc.contributor.authorCassady, Ren_HK
dc.contributor.authorHannam, Ven_HK
dc.contributor.authorAckerley, Cen_HK
dc.contributor.authorPost, Men_HK
dc.contributor.authorTanswell, AKen_HK
dc.contributor.authorOlek, Ken_HK
dc.contributor.authorO'Brodovich, Hen_HK
dc.contributor.authorTsui, LCen_HK
dc.date.accessioned2007-09-12T03:51:37Z-
dc.date.available2007-09-12T03:51:37Z-
dc.date.issued1998en_HK
dc.identifier.citationGene Therapy, 1998, v. 5 n. 2, p. 181-188en_HK
dc.identifier.issn0969-7128en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44335-
dc.description.abstractIntratracheal (i.t.) and intravenous (i.v.) delivery of DNA-vector formulations are two strategies to obtain gene transfer to the lung. It is still uncertain, however, which of these two modes of delivery will be more effective in the treatment of cystic fibrosis and other lung diseases. In this study, we attempted to optimize formulations of the cationic liposome DODAC:DOPE (dioleoyldimethylammoniumchloride:dioleoylphosphatidylethanolamine) complexed to plasmids encoding chloramphenicol acetyltransferase for i.t. and i.v. injection into CD-1 mice and compared the two methods. Our results showed that both methods conferred reporter gene expression in the lung that was significantly higher relative to injection of plasmid DNA alone. Expression using either mode of administration was maximal 24 h after injection and declined to around 10% of day 1 levels 2 weeks after injection. For i.v. delivery of DODAC:DOPE-DNA complexes multilamellar vesicles were more effective than large unilamellar vesicles in all organs investigated. Recombinant DNA could be detected in the distal lung region following either route of administration. However, i.t. administration predominantly led to DNA deposition in epithelial cells lining the bronchioles, eg in clara cells, whereas i.v. administration resulted in DNA deposition in the alveolar region of the lung including type II alveolar epithelial cells.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/gten_HK
dc.relation.ispartofGene Therapyen_HK
dc.subjectCationic liposomesen_HK
dc.subjectCystic fibrosisen_HK
dc.subjectLung gene therapyen_HK
dc.subject.meshLung gene therapyen_HK
dc.subject.meshCystic fibrosisen_HK
dc.subject.meshCationic liposomesen_HK
dc.subject.meshGene transfer techniquesen_HK
dc.subject.meshPhosphatidylethanolamines - administration & dosageen_HK
dc.titleComparison between intratracheal and intravenous administration of liposome-DNA complexes for cystic fibrosis lung gene therapyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0969-7128&volume=5&issue=2&spage=181&epage=188&date=1998&atitle=Comparison+between+intratracheal+and+intravenous+administration+of+liposome-DNA+complexes+for+cystic+fibrosis+lung+gene+therapyen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_OA_fulltexten_HK
dc.identifier.doi10.1038/sj.gt.3300562-
dc.identifier.pmid9578837-
dc.identifier.scopuseid_2-s2.0-6844258214en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-6844258214&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue2en_HK
dc.identifier.spage181en_HK
dc.identifier.epage188en_HK
dc.identifier.isiWOS:000072033100006-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridGriesenbach, U=6701826068en_HK
dc.identifier.scopusauthoridChonn, A=6701568752en_HK
dc.identifier.scopusauthoridCassady, R=6602198710en_HK
dc.identifier.scopusauthoridHannam, V=6603463916en_HK
dc.identifier.scopusauthoridAckerley, C=7006092057en_HK
dc.identifier.scopusauthoridPost, M=7201351734en_HK
dc.identifier.scopusauthoridTanswell, AK=35231349600en_HK
dc.identifier.scopusauthoridOlek, K=16186574200en_HK
dc.identifier.scopusauthoridO'Brodovich, H=7006287464en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats