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Article: Identification of an amplified gene cluster in glioma including two novel amplified genes isolated by exon trapping

TitleIdentification of an amplified gene cluster in glioma including two novel amplified genes isolated by exon trapping
Authors
Issue Date1997
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00439/index.htm
Citation
Human Genetics, 1997, v. 101 n. 2, p. 190-197 How to Cite?
AbstractGene amplification, which occurs in more than 50% of malignant gliomas, is considered to play a pivotal role in tumorigenesis. There are, however, few studies aimed toward the isolation of novel genes from amplified sequences. Previously, we reported amplification of the protooncogene MET (hepatocyte growth factor receptor; 7q31) in more than 20% of glioblastomas. For an approximate size estimation of the amplification unit we analyzed three glioblastomas all of which carried an amplified MET gene, by Southern blot analysis and/or competitive polymerase chain reaction using eight DNA markers. Although the extent of the amplified domain varied, the close vicinity of the MET gene was the only region consistently amplified in these glioblastomas. A yeast artificial chromosome (YAC) contig of 900 kb was refined spanning the amplified region flanking the MET gene. The YAC inserts were subcloned into 59 cosmids, which were used for exon trapping. Eight sequences were identical to parts of the genes MET and CAPZA2 (human actin capping protein cc-subunit). Two newly identified exons and the CAPZA2 exons were amplified in tumor TX3095, which retains an amplified MET gene. The new exons were localized close to MET and CAPZA2. Characterization of the clones, which were termed glioma-amplified sequence (GAS)7-1 and GAS7-2, showed an open reading frame and a different expression pattern in multiple human tissues. This study reports the identification of a cluster of amplified genes including two novel genes in a region amplified in more than 20% of glioblastomas.
Persistent Identifierhttp://hdl.handle.net/10722/44333
ISSN
2015 Impact Factor: 5.138
2015 SCImago Journal Rankings: 2.931
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMueller, HWen_HK
dc.contributor.authorMichel, Aen_HK
dc.contributor.authorHeckel, Den_HK
dc.contributor.authorFischer, Uen_HK
dc.contributor.authorTönnes, Men_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorScherer, Sen_HK
dc.contributor.authorZang, KDen_HK
dc.contributor.authorMeese, Een_HK
dc.date.accessioned2007-09-12T03:51:35Z-
dc.date.available2007-09-12T03:51:35Z-
dc.date.issued1997en_HK
dc.identifier.citationHuman Genetics, 1997, v. 101 n. 2, p. 190-197en_HK
dc.identifier.issn0340-6717en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44333-
dc.description.abstractGene amplification, which occurs in more than 50% of malignant gliomas, is considered to play a pivotal role in tumorigenesis. There are, however, few studies aimed toward the isolation of novel genes from amplified sequences. Previously, we reported amplification of the protooncogene MET (hepatocyte growth factor receptor; 7q31) in more than 20% of glioblastomas. For an approximate size estimation of the amplification unit we analyzed three glioblastomas all of which carried an amplified MET gene, by Southern blot analysis and/or competitive polymerase chain reaction using eight DNA markers. Although the extent of the amplified domain varied, the close vicinity of the MET gene was the only region consistently amplified in these glioblastomas. A yeast artificial chromosome (YAC) contig of 900 kb was refined spanning the amplified region flanking the MET gene. The YAC inserts were subcloned into 59 cosmids, which were used for exon trapping. Eight sequences were identical to parts of the genes MET and CAPZA2 (human actin capping protein cc-subunit). Two newly identified exons and the CAPZA2 exons were amplified in tumor TX3095, which retains an amplified MET gene. The new exons were localized close to MET and CAPZA2. Characterization of the clones, which were termed glioma-amplified sequence (GAS)7-1 and GAS7-2, showed an open reading frame and a different expression pattern in multiple human tissues. This study reports the identification of a cluster of amplified genes including two novel genes in a region amplified in more than 20% of glioblastomas.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00439/index.htmen_HK
dc.relation.ispartofHuman Geneticsen_HK
dc.rightsThe original publication is available at www.springerlink.comen_HK
dc.subject.meshChromosomes, human, pair 7en_HK
dc.subject.meshExons - geneticsen_HK
dc.subject.meshGene amplificationen_HK
dc.subject.meshGlioma - geneticsen_HK
dc.subject.meshMultigene familyen_HK
dc.titleIdentification of an amplified gene cluster in glioma including two novel amplified genes isolated by exon trappingen_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1007/s004390050612en_HK
dc.identifier.pmid9402967-
dc.identifier.scopuseid_2-s2.0-0031468813en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031468813&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume101en_HK
dc.identifier.issue2en_HK
dc.identifier.spage190en_HK
dc.identifier.epage197en_HK
dc.identifier.isiWOS:A1997YJ56000014-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridMueller, HW=7401833871en_HK
dc.identifier.scopusauthoridMichel, A=7201401602en_HK
dc.identifier.scopusauthoridHeckel, D=7003703578en_HK
dc.identifier.scopusauthoridFischer, U=22947094800en_HK
dc.identifier.scopusauthoridTönnes, M=7801559903en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridScherer, S=55159183300en_HK
dc.identifier.scopusauthoridZang, KD=7006166983en_HK
dc.identifier.scopusauthoridMeese, E=7004705747en_HK

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