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Article: Cloning, expression, and chromosomal localization to 11p12-13 of a human LIM/HOMEOBOX gene, hLim-1

TitleCloning, expression, and chromosomal localization to 11p12-13 of a human LIM/HOMEOBOX gene, hLim-1
Authors
Issue Date1997
PublisherMary Ann Liebert, Inc Publishers.
Citation
DNA and cell biology, 1997, v. 16 n. 6, p. 671-678 How to Cite?
AbstractWe have identified a putative transcription factor, designated hLim-1, from human fetal brain using degenerate polymerase chain reaction (PCR) and cDNA library screening. The deduced open reading frame, derived from sequencing a 3.0-kb hLim-1 cDNA, encodes a protein of 384 amino acids with two cysteine-rich LIM domains and one homeobox (HOX) DNA-binding domain. The nucleotide sequence of hLim-1 cDNA is 87% identical to mouse Lim-1 and the predicted amino acid sequence is greater than 97% conserved. Expression patterns of hLim-1 were evaluated by Northern analysis and reverse transcription (RT)-PCR coupled with Southern blotting. HLim-1 expression was observed in human brain, thymus, and tonsillar tissue. Expression of hLim-1 was also observed in 58% of acute myelogenous leukemia (AML) cell lines and in four of five primary samples from patients with chronic myeloid leukemia (CML) in myeloid blast transformation. The gene encoding hLim-1 was mapped using fluorescence in situ hybridization (FISH) to human chromosome 11p12-13. The expression pattern and structural characteristics of the hLim-1 gene suggest that it encodes a transcriptional regulatory protein involved in the control of differentiation and development of neural and lymphoid cells. Its expression in CML in blast crisis suggests that it may be involved with progression in this disease; a prospective study is required to confirm this.
Persistent Identifierhttp://hdl.handle.net/10722/44331
ISSN
2015 Impact Factor: 2.574
2015 SCImago Journal Rankings: 0.810
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDong, WFen_HK
dc.contributor.authorHeng, HHQen_HK
dc.contributor.authorLowsky, Ren_HK
dc.contributor.authorXu, Yen_HK
dc.contributor.authorDeCoteau, JFen_HK
dc.contributor.authorShi, XMen_HK
dc.contributor.authorTsui, L-Cen_HK
dc.contributor.authorMinden, MDen_HK
dc.date.accessioned2007-09-12T03:51:33Z-
dc.date.available2007-09-12T03:51:33Z-
dc.date.issued1997en_HK
dc.identifier.citationDNA and cell biology, 1997, v. 16 n. 6, p. 671-678en_HK
dc.identifier.issn1044-5498en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44331-
dc.description.abstractWe have identified a putative transcription factor, designated hLim-1, from human fetal brain using degenerate polymerase chain reaction (PCR) and cDNA library screening. The deduced open reading frame, derived from sequencing a 3.0-kb hLim-1 cDNA, encodes a protein of 384 amino acids with two cysteine-rich LIM domains and one homeobox (HOX) DNA-binding domain. The nucleotide sequence of hLim-1 cDNA is 87% identical to mouse Lim-1 and the predicted amino acid sequence is greater than 97% conserved. Expression patterns of hLim-1 were evaluated by Northern analysis and reverse transcription (RT)-PCR coupled with Southern blotting. HLim-1 expression was observed in human brain, thymus, and tonsillar tissue. Expression of hLim-1 was also observed in 58% of acute myelogenous leukemia (AML) cell lines and in four of five primary samples from patients with chronic myeloid leukemia (CML) in myeloid blast transformation. The gene encoding hLim-1 was mapped using fluorescence in situ hybridization (FISH) to human chromosome 11p12-13. The expression pattern and structural characteristics of the hLim-1 gene suggest that it encodes a transcriptional regulatory protein involved in the control of differentiation and development of neural and lymphoid cells. Its expression in CML in blast crisis suggests that it may be involved with progression in this disease; a prospective study is required to confirm this.en_HK
dc.languageengen_HK
dc.publisherMary Ann Liebert, Inc Publishers.en_HK
dc.relation.ispartofDNA and cell biology-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThis is a copy of an article published in the [DNA and cell biology] © [1997] [copyright Mary Ann Liebert, Inc.]; [DNA and cell biology] is available online at: http://www.liebertonline.com.-
dc.subject.meshChromosomes, human, pair 11en_HK
dc.subject.meshHomeodomain proteins - geneticsen_HK
dc.subject.meshIn situ hybridization, fluorescenceen_HK
dc.subject.meshSequence homology, amino aciden_HK
dc.subject.meshMolecular sequence dataen_HK
dc.titleCloning, expression, and chromosomal localization to 11p12-13 of a human LIM/HOMEOBOX gene, hLim-1en_HK
dc.typeArticleen_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1089/dna.1997.16.671-
dc.identifier.pmid9212161-
dc.identifier.scopuseid_2-s2.0-0030911692-
dc.identifier.isiWOS:A1997XJ16000001-

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