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Article: A panel of radiation hybrids defining the 7q31-q32 region of human chromosome 7

TitleA panel of radiation hybrids defining the 7q31-q32 region of human chromosome 7
Authors
KeywordsChromosome 7
Radiation hybrid
Sequence tagged sites (STS)
Issue Date1996
PublisherOxford University Press. The Journal's web site is located at http://dnaresearch.oxfordjournals.org
Citation
Dna Research, 1996, v. 3 n. 3, p. 181-183 How to Cite?
AbstractMouse A9 cells containing human chromosome 7 tagged with pSV2neo were irradiated with X-rays and fused to A9 cells to isolate G418-resistant clones. From these clones, we selected radiation hybrids that contained 10-40 Mb of human DNA apparently at a single site of their genome by FISH analysis using human repetitive sequences as a probe. Then we made a panel of hybrids that contained various fragments of the 7q31-q32 region and cover its entire region altogether by PCR with STS markers of human chromosome 7. This panel is useful in chromosome transfer experiments since the dominant selective marker neo gene is attached to human DNA.
Persistent Identifierhttp://hdl.handle.net/10722/44312
ISSN
2015 Impact Factor: 5.267
2015 SCImago Journal Rankings: 2.420
Other Identifiers
References

 

DC FieldValueLanguage
dc.contributor.authorNakabayashi, Ken_HK
dc.contributor.authorOgata, Ten_HK
dc.contributor.authorFujii, Men_HK
dc.contributor.authorTakahashi, EIen_HK
dc.contributor.authorOgino, Hen_HK
dc.contributor.authorMigihshita, Een_HK
dc.contributor.authorScherer, SWen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorAyusawa, Den_HK
dc.date.accessioned2007-09-12T03:51:12Z-
dc.date.available2007-09-12T03:51:12Z-
dc.date.issued1996en_HK
dc.identifierhttp://dnaresearch.oxfordjournals.org/cgi/reprint/3/3/181en_HK
dc.identifier.citationDna Research, 1996, v. 3 n. 3, p. 181-183en_HK
dc.identifier.issn1340-2838en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44312-
dc.description.abstractMouse A9 cells containing human chromosome 7 tagged with pSV2neo were irradiated with X-rays and fused to A9 cells to isolate G418-resistant clones. From these clones, we selected radiation hybrids that contained 10-40 Mb of human DNA apparently at a single site of their genome by FISH analysis using human repetitive sequences as a probe. Then we made a panel of hybrids that contained various fragments of the 7q31-q32 region and cover its entire region altogether by PCR with STS markers of human chromosome 7. This panel is useful in chromosome transfer experiments since the dominant selective marker neo gene is attached to human DNA.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://dnaresearch.oxfordjournals.orgen_HK
dc.relation.ispartofDNA Researchen_HK
dc.subjectChromosome 7en_HK
dc.subjectRadiation hybriden_HK
dc.subjectSequence tagged sites (STS)en_HK
dc.subject.meshChromosome 7en_HK
dc.subject.meshSequence tagged sites (sts)en_HK
dc.subject.meshRadiation hybriden_HK
dc.subject.meshHybrid cells - radiation effectsen_HK
dc.subject.meshIn situ hybridization, fluorescenceen_HK
dc.titleA panel of radiation hybrids defining the 7q31-q32 region of human chromosome 7en_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1093/dnares/3.3.181-
dc.identifier.pmid8905237-
dc.identifier.scopuseid_2-s2.0-0030606601en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030606601&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume3en_HK
dc.identifier.issue3en_HK
dc.identifier.spage181en_HK
dc.identifier.epage183en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridNakabayashi, K=7101927819en_HK
dc.identifier.scopusauthoridOgata, T=7402000687en_HK
dc.identifier.scopusauthoridFujii, M=7403351377en_HK
dc.identifier.scopusauthoridTakahashi, EI=35428825300en_HK
dc.identifier.scopusauthoridOgino, H=7201714283en_HK
dc.identifier.scopusauthoridMigihshita, E=8439729400en_HK
dc.identifier.scopusauthoridScherer, SW=35374654500en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridAyusawa, D=7006549610en_HK

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