Article: In vivo measurements of ion transport in long-living CF mice

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TitleIn vivo measurements of ion transport in long-living CF mice
AuthorsWilschanski, MA2 3
Rozmahel, R2 3
Beharry, S3
Kent, G3
Li, C3
Tsui, LC2 3
Durie, P2 3
Bear, CE1 3
Issue Date1996
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
CitationBiochemical And Biophysical Research Communications, 1996, v. 219 n. 3, p. 753-759 [How to Cite?]
DOI: http://dx.doi.org/10.1006/bbrc.1996.0306
AbstractThe Cftr (Cystic Fibrosis Transmembrane Conductance Regulator) gene codes for an epithelial chloride (C1) channel essential for fluid secretion into the respiratory and gastrointestinal tract and from exocrine glands. Mice lacking CFTR function due to a disruption of Cftr exon 10 or exon 1 (Cftr(mlUNC/MlUNC) or Cftr(mlHSC/mlHSC) mice, respectively) generally suffer from severe gastrointestinal disease resulting in death shortly after birth or at the time of weaning. However, a subgroup of the Cftr(mlHSC/mlHSC) mice have been characterized which exhibit relatively mild intestinal pathology resulting in a noncompromised lifespan compared to the more severely affected Cftr(mlUNC/mlUNC) mice. We compared the ion transport capacity of the intestinal mucosa of the mildly and severely affected CF mice using the in vivo technique of rectal potential difference (PD) measurement and found that the net calcium-activated chloride conductance toward the lumen was much greater in the rectum of mildly affected mice than in the severely affected mice. Hence, the milder phenotype may be related to the expression of a factor which enhances the net calcium-activated chloride conductance into the lumen of the intestinal tract.
ISSN0006-291X
2011 Impact Factor: 2.484
2011 SCImago Journal Rankings: 0.287
DOIhttp://dx.doi.org/10.1006/bbrc.1996.0306
ISI Accession Number IDWOS:A1996TZ79900014
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorWilschanski, MA
dc.contributor.authorRozmahel, R
dc.contributor.authorBeharry, S
dc.contributor.authorKent, G
dc.contributor.authorLi, C
dc.contributor.authorTsui, LC
dc.contributor.authorDurie, P
dc.contributor.authorBear, CE
dc.date.accessioned2007-09-12T03:50:56Z
dc.date.available2007-09-12T03:50:56Z
dc.date.issued1996
dc.description.abstractThe Cftr (Cystic Fibrosis Transmembrane Conductance Regulator) gene codes for an epithelial chloride (C1) channel essential for fluid secretion into the respiratory and gastrointestinal tract and from exocrine glands. Mice lacking CFTR function due to a disruption of Cftr exon 10 or exon 1 (Cftr(mlUNC/MlUNC) or Cftr(mlHSC/mlHSC) mice, respectively) generally suffer from severe gastrointestinal disease resulting in death shortly after birth or at the time of weaning. However, a subgroup of the Cftr(mlHSC/mlHSC) mice have been characterized which exhibit relatively mild intestinal pathology resulting in a noncompromised lifespan compared to the more severely affected Cftr(mlUNC/mlUNC) mice. We compared the ion transport capacity of the intestinal mucosa of the mildly and severely affected CF mice using the in vivo technique of rectal potential difference (PD) measurement and found that the net calcium-activated chloride conductance toward the lumen was much greater in the rectum of mildly affected mice than in the severely affected mice. Hence, the milder phenotype may be related to the expression of a factor which enhances the net calcium-activated chloride conductance into the lumen of the intestinal tract.
dc.description.natureabstract
dc.identifier.citationBiochemical And Biophysical Research Communications, 1996, v. 219 n. 3, p. 753-759 [How to Cite?]
DOI: http://dx.doi.org/10.1006/bbrc.1996.0306
dc.identifier.doihttp://dx.doi.org/10.1006/bbrc.1996.0306
dc.identifier.epage759
dc.identifier.isiWOS:A1996TZ79900014
dc.identifier.issn0006-291X
2011 Impact Factor: 2.484
2011 SCImago Journal Rankings: 0.287
dc.identifier.issue3
dc.identifier.openurl
dc.identifier.pmid8645253
dc.identifier.scopuseid_2-s2.0-0029869364
dc.identifier.spage753
dc.identifier.urihttp://hdl.handle.net/10722/44299
dc.identifier.volume219
dc.languageeng
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
dc.publisher.placeUnited States
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.relation.referencesReferences in Scopus
dc.subject.meshChlorides - metabolism
dc.subject.meshAmiloride - pharmacology
dc.subject.meshCystic fibrosis - genetics - metabolism - physiopathology
dc.subject.meshCystic fibrosis transmembrane conductance regulator - genetics - physiology
dc.subject.meshEpithelium - physiology - physiopathology
dc.titleIn vivo measurements of ion transport in long-living CF mice
dc.typeArticle
Author Affiliations
  1. University of Toronto, Faculty of Medicine
  2. University of Toronto
  3. Hospital for Sick Children, Toronto