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Article: In vivo measurements of ion transport in long-living CF mice

TitleIn vivo measurements of ion transport in long-living CF mice
Authors
Issue Date1996
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 1996, v. 219 n. 3, p. 753-759 How to Cite?
Abstract
The Cftr (Cystic Fibrosis Transmembrane Conductance Regulator) gene codes for an epithelial chloride (C1) channel essential for fluid secretion into the respiratory and gastrointestinal tract and from exocrine glands. Mice lacking CFTR function due to a disruption of Cftr exon 10 or exon 1 (Cftr(mlUNC/MlUNC) or Cftr(mlHSC/mlHSC) mice, respectively) generally suffer from severe gastrointestinal disease resulting in death shortly after birth or at the time of weaning. However, a subgroup of the Cftr(mlHSC/mlHSC) mice have been characterized which exhibit relatively mild intestinal pathology resulting in a noncompromised lifespan compared to the more severely affected Cftr(mlUNC/mlUNC) mice. We compared the ion transport capacity of the intestinal mucosa of the mildly and severely affected CF mice using the in vivo technique of rectal potential difference (PD) measurement and found that the net calcium-activated chloride conductance toward the lumen was much greater in the rectum of mildly affected mice than in the severely affected mice. Hence, the milder phenotype may be related to the expression of a factor which enhances the net calcium-activated chloride conductance into the lumen of the intestinal tract.
Persistent Identifierhttp://hdl.handle.net/10722/44299
ISSN
2013 Impact Factor: 2.281
ISI Accession Number ID
References

 

Author Affiliations
  1. University of Toronto Faculty of Medicine
  2. University of Toronto
  3. Hospital for Sick Children University of Toronto
DC FieldValueLanguage
dc.contributor.authorWilschanski, MAen_HK
dc.contributor.authorRozmahel, Ren_HK
dc.contributor.authorBeharry, Sen_HK
dc.contributor.authorKent, Gen_HK
dc.contributor.authorLi, Cen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorDurie, Pen_HK
dc.contributor.authorBear, CEen_HK
dc.date.accessioned2007-09-12T03:50:56Z-
dc.date.available2007-09-12T03:50:56Z-
dc.date.issued1996en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 1996, v. 219 n. 3, p. 753-759en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/44299-
dc.description.abstractThe Cftr (Cystic Fibrosis Transmembrane Conductance Regulator) gene codes for an epithelial chloride (C1) channel essential for fluid secretion into the respiratory and gastrointestinal tract and from exocrine glands. Mice lacking CFTR function due to a disruption of Cftr exon 10 or exon 1 (Cftr(mlUNC/MlUNC) or Cftr(mlHSC/mlHSC) mice, respectively) generally suffer from severe gastrointestinal disease resulting in death shortly after birth or at the time of weaning. However, a subgroup of the Cftr(mlHSC/mlHSC) mice have been characterized which exhibit relatively mild intestinal pathology resulting in a noncompromised lifespan compared to the more severely affected Cftr(mlUNC/mlUNC) mice. We compared the ion transport capacity of the intestinal mucosa of the mildly and severely affected CF mice using the in vivo technique of rectal potential difference (PD) measurement and found that the net calcium-activated chloride conductance toward the lumen was much greater in the rectum of mildly affected mice than in the severely affected mice. Hence, the milder phenotype may be related to the expression of a factor which enhances the net calcium-activated chloride conductance into the lumen of the intestinal tract.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subject.meshChlorides - metabolismen_HK
dc.subject.meshAmiloride - pharmacologyen_HK
dc.subject.meshCystic fibrosis - genetics - metabolism - physiopathologyen_HK
dc.subject.meshCystic fibrosis transmembrane conductance regulator - genetics - physiologyen_HK
dc.subject.meshEpithelium - physiology - physiopathologyen_HK
dc.titleIn vivo measurements of ion transport in long-living CF miceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=219&issue=3&spage=753&epage=759&date=1996&atitle=In+vivo+measurements+of+ion+transport+in+long-living+CF+miceen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.natureabstracten_HK
dc.identifier.doi10.1006/bbrc.1996.0306en_HK
dc.identifier.pmid8645253en_HK
dc.identifier.scopuseid_2-s2.0-0029869364en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029869364&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume219en_HK
dc.identifier.issue3en_HK
dc.identifier.spage753en_HK
dc.identifier.epage759en_HK
dc.identifier.isiWOS:A1996TZ79900014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWilschanski, MA=6701812857en_HK
dc.identifier.scopusauthoridRozmahel, R=6701510561en_HK
dc.identifier.scopusauthoridBeharry, S=6701596427en_HK
dc.identifier.scopusauthoridKent, G=34770664200en_HK
dc.identifier.scopusauthoridLi, C=7501673777en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridDurie, P=7005360997en_HK
dc.identifier.scopusauthoridBear, CE=7006718679en_HK

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