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Article: The cloning and chromosomal mapping of two novel human opioid- somatostatin-like receptor genes, GPR7 and GPR8, expressed in discrete areas of the brain

TitleThe cloning and chromosomal mapping of two novel human opioid- somatostatin-like receptor genes, GPR7 and GPR8, expressed in discrete areas of the brain
Authors
Issue Date1995
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygeno
Citation
Genomics, 1995, v. 28 n. 1, p. 84-91 How to Cite?
AbstractFollowing the cloning of the opioid receptors μ, κ, and δ, we conducted a search for related receptors. Using oligonucleotides based on the opioid and also the structurally related somatostatin receptors, we amplified genomic DNA using the polymerase chain reaction and isolated fragments of novel G protein-coupled receptor genes. Two of these gene fragments designated clones 12 and 11 were used to isolate the full-length genes. The intronless coding sequences of these genes, named GPR7 and GPR8, shared 70% identity with each other, and each shared significant similarity with the sequences encoding transmembrane regions of the opioid and somatostatin receptors. GPR7 was mapped to chromosome 10q11.2-q21.1 and GPR8 to chromosome 20q13.3. Northern blot analysis using human mRNA demonstrated expression of GPR7 mainly in cerebellum and frontal cortex, while GPR8 was located mainly in the frontal cortex. In situ hybridization revealed expression of GPR7 in the human pituitary. A partial sequence of the mouse orthologue of GPR7 was obtained, and in situ hybridization demonstrated expression in discrete nuclei of brain, namely suprachiasmatic, areuate, and ventromedial nuclei of hypothalamus. A stable cell line expressing the GPR7 gene was created, but expression levels of the receptor were low. The available pharmacology indicated binding to several opioid drugs such as bremazocine, levorphanol, and β-FNA, but not to the opioid receptor subtype-selective μ, δ, or κ agonists.
Persistent Identifierhttp://hdl.handle.net/10722/44285
ISSN
2015 Impact Factor: 2.386
2015 SCImago Journal Rankings: 1.281
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorO'Dowd, BFen_HK
dc.contributor.authorScheideler, MAen_HK
dc.contributor.authorNguyen, Ten_HK
dc.contributor.authorCheng, Ren_HK
dc.contributor.authorRasmussen, JSen_HK
dc.contributor.authorMarchese, Aen_HK
dc.contributor.authorZastawny, Ren_HK
dc.contributor.authorHeng, HHQen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorShi, Xen_HK
dc.contributor.authorAsa, Sen_HK
dc.contributor.authorPuy, Len_HK
dc.contributor.authorGeorge, SRen_HK
dc.date.accessioned2007-09-12T03:50:38Z-
dc.date.available2007-09-12T03:50:38Z-
dc.date.issued1995en_HK
dc.identifier.citationGenomics, 1995, v. 28 n. 1, p. 84-91en_HK
dc.identifier.issn0888-7543en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44285-
dc.description.abstractFollowing the cloning of the opioid receptors μ, κ, and δ, we conducted a search for related receptors. Using oligonucleotides based on the opioid and also the structurally related somatostatin receptors, we amplified genomic DNA using the polymerase chain reaction and isolated fragments of novel G protein-coupled receptor genes. Two of these gene fragments designated clones 12 and 11 were used to isolate the full-length genes. The intronless coding sequences of these genes, named GPR7 and GPR8, shared 70% identity with each other, and each shared significant similarity with the sequences encoding transmembrane regions of the opioid and somatostatin receptors. GPR7 was mapped to chromosome 10q11.2-q21.1 and GPR8 to chromosome 20q13.3. Northern blot analysis using human mRNA demonstrated expression of GPR7 mainly in cerebellum and frontal cortex, while GPR8 was located mainly in the frontal cortex. In situ hybridization revealed expression of GPR7 in the human pituitary. A partial sequence of the mouse orthologue of GPR7 was obtained, and in situ hybridization demonstrated expression in discrete nuclei of brain, namely suprachiasmatic, areuate, and ventromedial nuclei of hypothalamus. A stable cell line expressing the GPR7 gene was created, but expression levels of the receptor were low. The available pharmacology indicated binding to several opioid drugs such as bremazocine, levorphanol, and β-FNA, but not to the opioid receptor subtype-selective μ, δ, or κ agonists.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygenoen_HK
dc.relation.ispartofGenomicsen_HK
dc.subject.meshBrain - metabolismen_HK
dc.subject.meshChromosomes, human, pair 10en_HK
dc.subject.meshChromosomes, human, pair 14en_HK
dc.subject.meshChromosomes, human, pair 20en_HK
dc.subject.meshReceptors, opioid - genetics - metabolismen_HK
dc.titleThe cloning and chromosomal mapping of two novel human opioid- somatostatin-like receptor genes, GPR7 and GPR8, expressed in discrete areas of the brainen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0888-7543&volume=28&issue=1&spage=84&epage=91&date=1995&atitle=The+cloning+and+chromosomal+mapping+of+two+novel+human+opioid-somatostatin-like+receptor+genes+expressed+in+discrete+areas+of+the+brianen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1006/geno.1995.1109en_HK
dc.identifier.pmid7590751-
dc.identifier.scopuseid_2-s2.0-0029116382en_HK
dc.identifier.volume28en_HK
dc.identifier.issue1en_HK
dc.identifier.spage84en_HK
dc.identifier.epage91en_HK
dc.identifier.isiWOS:A1995RL09600011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridO'Dowd, BF=7102778005en_HK
dc.identifier.scopusauthoridScheideler, MA=35820835600en_HK
dc.identifier.scopusauthoridNguyen, T=26667936100en_HK
dc.identifier.scopusauthoridCheng, R=7201955297en_HK
dc.identifier.scopusauthoridRasmussen, JS=7402620551en_HK
dc.identifier.scopusauthoridMarchese, A=7005075855en_HK
dc.identifier.scopusauthoridZastawny, R=6603100120en_HK
dc.identifier.scopusauthoridHeng, HHQ=7005338076en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridShi, X=7402953863en_HK
dc.identifier.scopusauthoridAsa, S=7103083314en_HK
dc.identifier.scopusauthoridPuy, L=6701570001en_HK
dc.identifier.scopusauthoridGeorge, SR=35429392100en_HK

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