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Article: Localization to chromosome 7q36.1 of the human XRCC2 gene, determining sensitivity to DNA-damaging agents

TitleLocalization to chromosome 7q36.1 of the human XRCC2 gene, determining sensitivity to DNA-damaging agents
Authors
Issue Date1995
PublisherOxford University Press. The Journal's web site is located at http://hmg.oxfordjournals.org/
Citation
Human Molecular Genetics, 1995, v. 4 n. 1, p. 113-120 How to Cite?
AbstractThe identification of genes controlling cellular response to DNA damage is of considerable importance, and cell lines showing hypersensitivity to DNA-damaging agents can be used as vehicles to map and clone these genes. In this study the hamster cell line irs1, showing hypersensitivity to a number of different DNA-damaging agents, was fused to normal human cells to complement the defect. The resultant hybrids were analysed by Alu-PCR, chromosome painting, and with DNA markers to map the complementing gene (named XRCC2) to a specific chromosomal region. These hybrids showed correction of sensitivity to both X-rays and to mitomycin-C, and contained human chromosome 7, often as their only human component. Hybrids showing unstable retention of human chromosomes were sub-cloned to show that loss of chromosome 7 and loss of resistance to mitomycin-C occurred concordantly. Two separate hybrids were found to have a smaller piece of chromosome 7, and specific DNA probes and microsatellite markers defined this as a contiguous region at 7q35-36. Hybrid irradiation-fusion methods were used to further reduce the size of the complementing genomic region and to localize the gene to an approximately 3-5 Mb region at 7q36.1.
Persistent Identifierhttp://hdl.handle.net/10722/44282
ISSN
2015 Impact Factor: 5.985
2015 SCImago Journal Rankings: 4.288
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorThacker, Jen_HK
dc.contributor.authorTambini, CEen_HK
dc.contributor.authorSimpson, PJen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorScherer, SWen_HK
dc.date.accessioned2007-09-12T03:50:35Z-
dc.date.available2007-09-12T03:50:35Z-
dc.date.issued1995en_HK
dc.identifier.citationHuman Molecular Genetics, 1995, v. 4 n. 1, p. 113-120en_HK
dc.identifier.issn0964-6906en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44282-
dc.description.abstractThe identification of genes controlling cellular response to DNA damage is of considerable importance, and cell lines showing hypersensitivity to DNA-damaging agents can be used as vehicles to map and clone these genes. In this study the hamster cell line irs1, showing hypersensitivity to a number of different DNA-damaging agents, was fused to normal human cells to complement the defect. The resultant hybrids were analysed by Alu-PCR, chromosome painting, and with DNA markers to map the complementing gene (named XRCC2) to a specific chromosomal region. These hybrids showed correction of sensitivity to both X-rays and to mitomycin-C, and contained human chromosome 7, often as their only human component. Hybrids showing unstable retention of human chromosomes were sub-cloned to show that loss of chromosome 7 and loss of resistance to mitomycin-C occurred concordantly. Two separate hybrids were found to have a smaller piece of chromosome 7, and specific DNA probes and microsatellite markers defined this as a contiguous region at 7q35-36. Hybrid irradiation-fusion methods were used to further reduce the size of the complementing genomic region and to localize the gene to an approximately 3-5 Mb region at 7q36.1.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://hmg.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Molecular Geneticsen_HK
dc.subject.meshChromosomes, human, pair 7en_HK
dc.subject.meshDna - drug effects - radiation effectsen_HK
dc.subject.meshDna damageen_HK
dc.subject.meshGenetic complementation testen_HK
dc.subject.meshMitomycin - toxicityen_HK
dc.titleLocalization to chromosome 7q36.1 of the human XRCC2 gene, determining sensitivity to DNA-damaging agentsen_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1093/hmg/4.1.113-
dc.identifier.pmid7711722-
dc.identifier.scopuseid_2-s2.0-0028888636en_HK
dc.identifier.volume4en_HK
dc.identifier.issue1en_HK
dc.identifier.spage113en_HK
dc.identifier.epage120en_HK
dc.identifier.isiWOS:A1995QD37100016-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridThacker, J=7101915467en_HK
dc.identifier.scopusauthoridTambini, CE=6506020572en_HK
dc.identifier.scopusauthoridSimpson, PJ=16172630300en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridScherer, SW=35374654500en_HK

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