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Article: Organization of heterologous DNA inserts on the mouse meiotic chromosome core

TitleOrganization of heterologous DNA inserts on the mouse meiotic chromosome core
Authors
Issue Date1994
PublisherSpringer Verlag. The Journal's web site is located at http://www.springeronline.com/sgw/cda/frontpage/0,10735,5-40109-70-1066702-0,00.html
Citation
Chromosoma, 1994, v. 103 n. 6, p. 401-407 How to Cite?
AbstractWith simultaneous immunofluorescence and fluorescent in situ hybridization, we have determined the organization of native and heterologous DNA sequences relative to the cores of meiotic prophase chromosomes. The normal chromatin organization is demonstrated with probes of mouse sequences: a cosmid probe that identifies unique sequences and a 720 kb yeast artificial chromosome (YAC) probe that recognizes a specific region of the chromatin domain. The heterologous DNA consists of a 1.8 Mb insertion of 40 tandem head-to-tail phage λ LIZ vectors and of 11.4 Mb of bacterial/mouse DNA repeats. The lengthy λ insert is unusual in that it is not contained in the chromatin domain of chromosome 4 and in that it fails to form direct attachments to the chromosome core. The ends are attached indirectly, probably by means of the flanking mouse sequences. At late stages of meiotic prophase, while the terminal attachments remain the same, the λ DNA becomes highly compacted. Apparently, higher order condensation and core attachment are independent processes. The condensed inserts relax precociously at metaphase I. In the mouse heterozygous for the insert, the two sister inserts are usually merged, as are all four inserts in the homozygous mouse. Evidently chromatin loops with identical sequences can become associated during meiotic prophase. Mouse sequences within a heterologous DNA insert (repeats of bacterial plasmid pBR322 with a mouse β-globin insert) were observed to restore some degree of core attachment.
Persistent Identifierhttp://hdl.handle.net/10722/44278
ISSN
2015 Impact Factor: 4.303
2015 SCImago Journal Rankings: 3.397
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHeng, HHQen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorMoens, PBen_HK
dc.date.accessioned2007-09-12T03:50:30Z-
dc.date.available2007-09-12T03:50:30Z-
dc.date.issued1994en_HK
dc.identifier.citationChromosoma, 1994, v. 103 n. 6, p. 401-407en_HK
dc.identifier.issn0009-5915en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44278-
dc.description.abstractWith simultaneous immunofluorescence and fluorescent in situ hybridization, we have determined the organization of native and heterologous DNA sequences relative to the cores of meiotic prophase chromosomes. The normal chromatin organization is demonstrated with probes of mouse sequences: a cosmid probe that identifies unique sequences and a 720 kb yeast artificial chromosome (YAC) probe that recognizes a specific region of the chromatin domain. The heterologous DNA consists of a 1.8 Mb insertion of 40 tandem head-to-tail phage λ LIZ vectors and of 11.4 Mb of bacterial/mouse DNA repeats. The lengthy λ insert is unusual in that it is not contained in the chromatin domain of chromosome 4 and in that it fails to form direct attachments to the chromosome core. The ends are attached indirectly, probably by means of the flanking mouse sequences. At late stages of meiotic prophase, while the terminal attachments remain the same, the λ DNA becomes highly compacted. Apparently, higher order condensation and core attachment are independent processes. The condensed inserts relax precociously at metaphase I. In the mouse heterozygous for the insert, the two sister inserts are usually merged, as are all four inserts in the homozygous mouse. Evidently chromatin loops with identical sequences can become associated during meiotic prophase. Mouse sequences within a heterologous DNA insert (repeats of bacterial plasmid pBR322 with a mouse β-globin insert) were observed to restore some degree of core attachment.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://www.springeronline.com/sgw/cda/frontpage/0,10735,5-40109-70-1066702-0,00.htmlen_HK
dc.relation.ispartofChromosomaen_HK
dc.rightsThe original publication is available at www.springerlink.comen_HK
dc.subject.meshBacteriophage lambda - geneticsen_HK
dc.subject.meshChromosomes - geneticsen_HK
dc.subject.meshDna, recombinant - geneticsen_HK
dc.subject.meshDna, viral - geneticsen_HK
dc.subject.meshFluorescent antibody techniqueen_HK
dc.titleOrganization of heterologous DNA inserts on the mouse meiotic chromosome coreen_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1007/s004120050048en_HK
dc.identifier.pmid7859560-
dc.identifier.scopuseid_2-s2.0-0028004439en_HK
dc.identifier.volume103en_HK
dc.identifier.issue6en_HK
dc.identifier.spage401en_HK
dc.identifier.epage407en_HK
dc.identifier.isiWOS:A1994PT81500004-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridHeng, HHQ=7005338076en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridMoens, PB=35557314900en_HK

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