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Article: Regional localization of 725 human chromosome 7-specific yeast artificial chromosome clones

TitleRegional localization of 725 human chromosome 7-specific yeast artificial chromosome clones
Authors
Issue Date1994
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygeno
Citation
Genomics, 1994, v. 22 n. 2, p. 439-448 How to Cite?
AbstractToward the construction of a complete physical map of human chromosome 7, we have localized 725 YAC clones to cytogenetically defined regions using fluorescence in situ hybridization (FISH) and by screening with DNA markers of known chromosomal locations. These chromosome 7-specific YAC clones are part of a library constructed with DNA isolated from monochromosomal 7 human- hamster somatic cell hybrid lines. The FISH mapping for 575 clones was accomplished by using 'Alu-PCR' amplified YAC DNA against metaphase chromosome spreads made from a monochromosomal 7 human-mouse somatic cell hybrid line. Hybridization- or PCR-based screening of previously mapped DNA markers was performed for the mapping of 221 YAC clones. There was excellent correlation between the map locations obtained for the 71 YACs localized with both methods. All of the regionally localized YAC clones are valuable reagents for mapping and identification of disease genes on human chromosome 7.
Persistent Identifierhttp://hdl.handle.net/10722/44273
ISSN
2015 Impact Factor: 2.386
2015 SCImago Journal Rankings: 1.281
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKunz, Jen_HK
dc.contributor.authorScherer, SWen_HK
dc.contributor.authorKlawitz, Ien_HK
dc.contributor.authorSoder, Sen_HK
dc.contributor.authorDu, YZen_HK
dc.contributor.authorSpeich, Nen_HK
dc.contributor.authorKalffSuske, Men_HK
dc.contributor.authorHeng, HHQen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorGrzeschik, KHen_HK
dc.date.accessioned2007-09-12T03:50:23Z-
dc.date.available2007-09-12T03:50:23Z-
dc.date.issued1994en_HK
dc.identifier.citationGenomics, 1994, v. 22 n. 2, p. 439-448en_HK
dc.identifier.issn0888-7543en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44273-
dc.description.abstractToward the construction of a complete physical map of human chromosome 7, we have localized 725 YAC clones to cytogenetically defined regions using fluorescence in situ hybridization (FISH) and by screening with DNA markers of known chromosomal locations. These chromosome 7-specific YAC clones are part of a library constructed with DNA isolated from monochromosomal 7 human- hamster somatic cell hybrid lines. The FISH mapping for 575 clones was accomplished by using 'Alu-PCR' amplified YAC DNA against metaphase chromosome spreads made from a monochromosomal 7 human-mouse somatic cell hybrid line. Hybridization- or PCR-based screening of previously mapped DNA markers was performed for the mapping of 221 YAC clones. There was excellent correlation between the map locations obtained for the 71 YACs localized with both methods. All of the regionally localized YAC clones are valuable reagents for mapping and identification of disease genes on human chromosome 7.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygenoen_HK
dc.relation.ispartofGenomicsen_HK
dc.subject.meshChromosomes, artificial, yeasten_HK
dc.subject.meshChromosomes, human, pair 7en_HK
dc.subject.meshGenetic markersen_HK
dc.subject.meshIn situ hybridization, fluorescenceen_HK
dc.subject.meshPolymerase chain reactionen_HK
dc.titleRegional localization of 725 human chromosome 7-specific yeast artificial chromosome clonesen_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1006/geno.1994.1407en_HK
dc.identifier.pmid7806232-
dc.identifier.scopuseid_2-s2.0-0028169989en_HK
dc.identifier.volume22en_HK
dc.identifier.issue2en_HK
dc.identifier.spage439en_HK
dc.identifier.epage448en_HK
dc.identifier.isiWOS:A1994PB57100026-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKunz, J=7102122018en_HK
dc.identifier.scopusauthoridScherer, SW=35374654500en_HK
dc.identifier.scopusauthoridKlawitz, I=6508184641en_HK
dc.identifier.scopusauthoridSoder, S=6602121375en_HK
dc.identifier.scopusauthoridDu, YZ=7402893752en_HK
dc.identifier.scopusauthoridSpeich, N=36943916900en_HK
dc.identifier.scopusauthoridKalffSuske, M=6602127306en_HK
dc.identifier.scopusauthoridHeng, HHQ=7005338076en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridGrzeschik, KH=7004321252en_HK

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