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Article: Multi-ion pore behaviour in the CFTR chloride channel

TitleMulti-ion pore behaviour in the CFTR chloride channel
Authors
Issue Date1993
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/nature
Citation
Nature, 1993, v. 366 n. 6450, p. 79-82 How to Cite?
AbstractCYSTIC fibrosis transmembrane conductance regulator (CFTR) is a non- rectifying, low-conductance channel regulated by ATP and phosphorylation, which mediates apical chloride conductance in secretory epithelia and malfunctions in cystic fibrosis (CF). Mutations at Lys 335 and Arg 347 in the sixth predicted transmembrane helix of CFTR alter its halide selectivity in whole-cell studies and its single channel conductance, but the physical basis of these alterations is unknown and permeation in CFTR is poorly understood. Here we present evidence that wild-type CFTR can contain more than one anion simultaneously. The conductance of CFTR passes through a minimum when channels are bathed in mixtures of two permeant anions. This anomalous mole fraction effect can be abolished by replacing Arg 347 with an aspartate and can be toggled on or off by varying the pH after the same residue is replaced with a histidine. Thus the CFTR channel should provide a convenient model in which to study multi-ion pore behaviour and conduction. The loss of multiple occupancy may explain how naturally occurring CF mutations at this site cause disease.
Persistent Identifierhttp://hdl.handle.net/10722/44266
ISSN
2015 Impact Factor: 38.138
2015 SCImago Journal Rankings: 21.936
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTabcharani, JAen_HK
dc.contributor.authorRommens, JMen_HK
dc.contributor.authorHou, YXen_HK
dc.contributor.authorChang, XBen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorRiordan, JRen_HK
dc.contributor.authorHanrahan, JWen_HK
dc.date.accessioned2007-09-12T03:50:15Z-
dc.date.available2007-09-12T03:50:15Z-
dc.date.issued1993en_HK
dc.identifier.citationNature, 1993, v. 366 n. 6450, p. 79-82en_HK
dc.identifier.issn0028-0836en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44266-
dc.description.abstractCYSTIC fibrosis transmembrane conductance regulator (CFTR) is a non- rectifying, low-conductance channel regulated by ATP and phosphorylation, which mediates apical chloride conductance in secretory epithelia and malfunctions in cystic fibrosis (CF). Mutations at Lys 335 and Arg 347 in the sixth predicted transmembrane helix of CFTR alter its halide selectivity in whole-cell studies and its single channel conductance, but the physical basis of these alterations is unknown and permeation in CFTR is poorly understood. Here we present evidence that wild-type CFTR can contain more than one anion simultaneously. The conductance of CFTR passes through a minimum when channels are bathed in mixtures of two permeant anions. This anomalous mole fraction effect can be abolished by replacing Arg 347 with an aspartate and can be toggled on or off by varying the pH after the same residue is replaced with a histidine. Thus the CFTR channel should provide a convenient model in which to study multi-ion pore behaviour and conduction. The loss of multiple occupancy may explain how naturally occurring CF mutations at this site cause disease.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/natureen_HK
dc.relation.ispartofNatureen_HK
dc.subject.meshChloride channels - genetics - physiologyen_HK
dc.subject.meshCystic fibrosis - metabolismen_HK
dc.subject.meshIon channels - genetics - physiologyen_HK
dc.subject.meshMembrane proteins - genetics - physiologyen_HK
dc.subject.meshThiocyanates - metabolismen_HK
dc.titleMulti-ion pore behaviour in the CFTR chloride channelen_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1038/366079a0en_HK
dc.identifier.pmid7694154-
dc.identifier.scopuseid_2-s2.0-0027423190en_HK
dc.identifier.volume366en_HK
dc.identifier.issue6450en_HK
dc.identifier.spage79en_HK
dc.identifier.epage82en_HK
dc.identifier.isiWOS:A1993MF00700059-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTabcharani, JA=6701582734en_HK
dc.identifier.scopusauthoridRommens, JM=7006884140en_HK
dc.identifier.scopusauthoridHou, YX=36771685300en_HK
dc.identifier.scopusauthoridChang, XB=25934062500en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridRiordan, JR=7202229758en_HK
dc.identifier.scopusauthoridHanrahan, JW=7103386837en_HK

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