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Article: Structure and chromosomal localization of the human constitutive endothelial nitric oxide synthase gene

TitleStructure and chromosomal localization of the human constitutive endothelial nitric oxide synthase gene
Authors
Issue Date1993
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1993, v. 268 n. 23, p. 17478-17488 How to Cite?
AbstractEndothelial nitric oxide (NO) synthase is a unique NO synthase isoform that is expressed constitutively by vascular endothelium both in vivo and in vitro and is believed essential to local vascular homeostasis. This calcium/calmodulin-dependent isoform is distinct from neuronal NO synthase. Genomic clones encoding the human endothelial NO synthase were isolated and the structural organization of the gene was determined. The gene contains 26 exons spanning ≃21 kilobases of genomic DNA, encodes a messenger RNA of 4052 nucleotides, and is present as a single copy in the haploid human genome. Characterization of 5'-flanking genomic regions indicates that the endothelial NO synthase promoter is 'TATA-less' and exhibits proximal promoter elements consistent with a constitutively expressed gene that is found in endothelial cells, namely Sp1 and GATA motifs. The 5'-flanking region contains putative AP-1, AP-2, NF-1, heavy metal, acute-phase response shear stress, and sterol-regulatory cis-elements. The human endothelial NO synthase gene was assigned to the 7q35 → 7q36 region of chromosome 7 by Southern blot hybridization of human-rodent somatic cell hybrid lines and fluorescence in situ hybridization, whereas human neuronal NO synthase localized to the 12q24.2 region of chromosome 12.
Persistent Identifierhttp://hdl.handle.net/10722/44265
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
Other Identifiers
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMarsden, PAen_HK
dc.contributor.authorHeng, HHQen_HK
dc.contributor.authorScherer, SWen_HK
dc.contributor.authorStewart, RJen_HK
dc.contributor.authorHall, AVen_HK
dc.contributor.authorShi , XMen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorSchappert, KTen_HK
dc.date.accessioned2007-09-12T03:50:13Z-
dc.date.available2007-09-12T03:50:13Z-
dc.date.issued1993en_HK
dc.identifierhttp://www.jbc.org/cgi/reprint/268/23/17478.pdfen_HK
dc.identifier.citationJournal Of Biological Chemistry, 1993, v. 268 n. 23, p. 17478-17488en_HK
dc.identifier.issn0021-9258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44265-
dc.description.abstractEndothelial nitric oxide (NO) synthase is a unique NO synthase isoform that is expressed constitutively by vascular endothelium both in vivo and in vitro and is believed essential to local vascular homeostasis. This calcium/calmodulin-dependent isoform is distinct from neuronal NO synthase. Genomic clones encoding the human endothelial NO synthase were isolated and the structural organization of the gene was determined. The gene contains 26 exons spanning ≃21 kilobases of genomic DNA, encodes a messenger RNA of 4052 nucleotides, and is present as a single copy in the haploid human genome. Characterization of 5'-flanking genomic regions indicates that the endothelial NO synthase promoter is 'TATA-less' and exhibits proximal promoter elements consistent with a constitutively expressed gene that is found in endothelial cells, namely Sp1 and GATA motifs. The 5'-flanking region contains putative AP-1, AP-2, NF-1, heavy metal, acute-phase response shear stress, and sterol-regulatory cis-elements. The human endothelial NO synthase gene was assigned to the 7q35 → 7q36 region of chromosome 7 by Southern blot hybridization of human-rodent somatic cell hybrid lines and fluorescence in situ hybridization, whereas human neuronal NO synthase localized to the 12q24.2 region of chromosome 12.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_HK
dc.relation.ispartofJournal of Biological Chemistryen_HK
dc.rightsJournal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc.en_HK
dc.subject.meshChromosomes, human, pair 7en_HK
dc.subject.meshEndothelium, vascular - enzymologyen_HK
dc.subject.meshNitric oxide synthaseen_HK
dc.subject.meshRepetitive sequences, nucleic aciden_HK
dc.subject.meshIntronsen_HK
dc.titleStructure and chromosomal localization of the human constitutive endothelial nitric oxide synthase geneen_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_OA_fulltexten_HK
dc.identifier.pmid7688726-
dc.identifier.scopuseid_2-s2.0-0027183208en_HK
dc.identifier.volume268en_HK
dc.identifier.issue23en_HK
dc.identifier.spage17478en_HK
dc.identifier.epage17488en_HK
dc.identifier.isiWOS:A1993LQ98800087-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMarsden, PA=7201783899en_HK
dc.identifier.scopusauthoridHeng, HHQ=7005338076en_HK
dc.identifier.scopusauthoridScherer, SW=35374654500en_HK
dc.identifier.scopusauthoridStewart, RJ=35557271400en_HK
dc.identifier.scopusauthoridHall, AV=7403288758en_HK
dc.identifier.scopusauthoridShi , XM=37043617300en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridSchappert, KT=35884962200en_HK

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