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Article: Modes of DAPI banding and simultaneous in situ hybridization

TitleModes of DAPI banding and simultaneous in situ hybridization
Authors
Issue Date1993
PublisherSpringer Verlag. The Journal's web site is located at http://www.springeronline.com/sgw/cda/frontpage/0,10735,5-40109-70-1066702-0,00.html
Citation
Chromosoma, 1993, v. 102 n. 5, p. 325-332 How to Cite?
AbstractBy controlling the degree of chromatin denaturation through formamide incubation, or by heat treatment and/or by high pH, three types of high quality 4',6-diamidino-2-phenylindole (DAPI) bands can be produced sequentially on the same set of 5-bromo-2'-deoxyuridine (BrdU)-incorporated chromosomes: first DAPI multibanding (the equivalent of Q-banding), then partial C-banding including distamycin A (DA)/DAPI banding, and finally C-banding pattern. It is assumed that the different DAPI-chromatin interactions following these treatments reflect the different chromatin structures at the chromosomal sites. Since the DAPI banding protocol is compatible with in situ hybridization, the combination of fluorescent in situ hybridization (FISH) with DAPI banding allows the simultaneous detection of signals from the DNA probes and the identification of the chromosomal band location of the probe. We demonstrate this useful application with the localization of the cystic fibrosis and Duchenne muscular dystrophy gene probes to their appropriate bands.
Persistent Identifierhttp://hdl.handle.net/10722/44261
ISSN
2015 Impact Factor: 4.303
2015 SCImago Journal Rankings: 3.397
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHeng, HHQen_HK
dc.contributor.authorTsui, LCen_HK
dc.date.accessioned2007-09-12T03:50:07Z-
dc.date.available2007-09-12T03:50:07Z-
dc.date.issued1993en_HK
dc.identifier.citationChromosoma, 1993, v. 102 n. 5, p. 325-332en_HK
dc.identifier.issn0009-5915en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44261-
dc.description.abstractBy controlling the degree of chromatin denaturation through formamide incubation, or by heat treatment and/or by high pH, three types of high quality 4',6-diamidino-2-phenylindole (DAPI) bands can be produced sequentially on the same set of 5-bromo-2'-deoxyuridine (BrdU)-incorporated chromosomes: first DAPI multibanding (the equivalent of Q-banding), then partial C-banding including distamycin A (DA)/DAPI banding, and finally C-banding pattern. It is assumed that the different DAPI-chromatin interactions following these treatments reflect the different chromatin structures at the chromosomal sites. Since the DAPI banding protocol is compatible with in situ hybridization, the combination of fluorescent in situ hybridization (FISH) with DAPI banding allows the simultaneous detection of signals from the DNA probes and the identification of the chromosomal band location of the probe. We demonstrate this useful application with the localization of the cystic fibrosis and Duchenne muscular dystrophy gene probes to their appropriate bands.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://www.springeronline.com/sgw/cda/frontpage/0,10735,5-40109-70-1066702-0,00.htmlen_HK
dc.relation.ispartofChromosomaen_HK
dc.rightsThe original publication is available at www.springerlink.comen_HK
dc.subject.meshCells, cultureden_HK
dc.subject.meshChromosome banding - methodsen_HK
dc.subject.meshCystic fibrosis - geneticsen_HK
dc.subject.meshFluorescent dyesen_HK
dc.subject.meshHydrogen-ion concentrationen_HK
dc.titleModes of DAPI banding and simultaneous in situ hybridizationen_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1007/BF00661275en_HK
dc.identifier.pmid8325164-
dc.identifier.scopuseid_2-s2.0-0027225483en_HK
dc.identifier.volume102en_HK
dc.identifier.issue5en_HK
dc.identifier.spage325en_HK
dc.identifier.epage332en_HK
dc.identifier.isiWOS:A1993LB42300004-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridHeng, HHQ=7005338076en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK

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