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Article: An everted repeat mediates retinoic acid induction of the γF-crystallin gene: Evidence of a direct role for retinoids in lens development

TitleAn everted repeat mediates retinoic acid induction of the γF-crystallin gene: Evidence of a direct role for retinoids in lens development
Authors
KeywordsCrystallin
Eye
Lens
Mouse
Nuclear receptor
Retinoic acid
Issue Date1993
PublisherCold Spring Harbor Laboratory Press. The Journal's web site is located at http://genesdev.cshlp.org/
Citation
Genes And Development, 1993, v. 7 n. 2, p. 295-307 How to Cite?
AbstractThe vertebrate lens is a classical system for examining mechanisms of tissue determination and differentiation, yet little is known about the signaling molecules controlling its development. Here, we report that retinoic acid (RA), a substance known for its teratogenic effects on the eye and as a natural endogenous morphogenetic agent, acts as a regulator of gene expression in the lens. We have identified a novel type of RA response element (RARE) within the lens-specific mouse γF-crystallin promoter, consisting of two (A/G)GGTCA motifs in an everted arrangement spaced by 8 nucleotides. This element (γF-RARE) mediates activation of the γF-crystallin promoter by ligand-activated endogenous lens cell RA receptors (RARs) and confers RA responsiveness when linked to a heterologous promoter. γF-RARE is bound in vitro by RAR/RXR heterodimers, and both receptors cooperate in vivo to trans-activate this element. These observations demonstrate a direct effect of RA on lens-specific gene expression and reveal a novel role for retinoids in the development and homeostasis of the mammalian eye.
Persistent Identifierhttp://hdl.handle.net/10722/44257
ISSN
2015 Impact Factor: 10.042
2015 SCImago Journal Rankings: 11.812
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTini, Men_HK
dc.contributor.authorOtulakowski, Gen_HK
dc.contributor.authorBreitmanen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorGiguere, Ven_HK
dc.date.accessioned2007-09-12T03:50:02Z-
dc.date.available2007-09-12T03:50:02Z-
dc.date.issued1993en_HK
dc.identifier.citationGenes And Development, 1993, v. 7 n. 2, p. 295-307en_HK
dc.identifier.issn0890-9369en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44257-
dc.description.abstractThe vertebrate lens is a classical system for examining mechanisms of tissue determination and differentiation, yet little is known about the signaling molecules controlling its development. Here, we report that retinoic acid (RA), a substance known for its teratogenic effects on the eye and as a natural endogenous morphogenetic agent, acts as a regulator of gene expression in the lens. We have identified a novel type of RA response element (RARE) within the lens-specific mouse γF-crystallin promoter, consisting of two (A/G)GGTCA motifs in an everted arrangement spaced by 8 nucleotides. This element (γF-RARE) mediates activation of the γF-crystallin promoter by ligand-activated endogenous lens cell RA receptors (RARs) and confers RA responsiveness when linked to a heterologous promoter. γF-RARE is bound in vitro by RAR/RXR heterodimers, and both receptors cooperate in vivo to trans-activate this element. These observations demonstrate a direct effect of RA on lens-specific gene expression and reveal a novel role for retinoids in the development and homeostasis of the mammalian eye.en_HK
dc.languageengen_HK
dc.publisherCold Spring Harbor Laboratory Press. The Journal's web site is located at http://genesdev.cshlp.org/en_HK
dc.relation.ispartofGenes and Developmenten_HK
dc.subjectCrystallinen_HK
dc.subjectEyeen_HK
dc.subjectLensen_HK
dc.subjectMouseen_HK
dc.subjectNuclear receptoren_HK
dc.subjectRetinoic aciden_HK
dc.subject.meshCrystallins - geneticsen_HK
dc.subject.meshLens, Crystalline - embryologyen_HK
dc.subject.meshTretinoin - metabolismen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshRepetitive Sequences, Nucleic Aciden_HK
dc.titleAn everted repeat mediates retinoic acid induction of the γF-crystallin gene: Evidence of a direct role for retinoids in lens developmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0890-9369&volume=7&issue=2&spage=295&epage=307&date=1993&atitle=An+everted+repeat+mediates+retinoic+acid+induction+of+the+gamma+F-crystallin+gene:+evidence+of+a+direct+role+for+retinoids+in+lens+developmenten_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_OA_fulltexten_HK
dc.identifier.doi10.1101/gad.7.2.295-
dc.identifier.pmid8436299-
dc.identifier.scopuseid_2-s2.0-0027409151en_HK
dc.identifier.volume7en_HK
dc.identifier.issue2en_HK
dc.identifier.spage295en_HK
dc.identifier.epage307en_HK
dc.identifier.isiWOS:A1993KM85900013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTini, M=55409977200en_HK
dc.identifier.scopusauthoridOtulakowski, G=6701369894en_HK
dc.identifier.scopusauthoridBreitman=7409717085en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridGiguere, V=7006252444en_HK

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