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Article: High-resolution mapping of mammalian genes by in situ hybridization to free chromatin

TitleHigh-resolution mapping of mammalian genes by in situ hybridization to free chromatin
Authors
Issue Date1992
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings Of The National Academy Of Sciences Of The United States Of America, 1992, v. 89 n. 20, p. 9509-9513 How to Cite?
AbstractFluorescence in situ hybridization to metaphase chromosomes or chromatin fibers in interphase nuclei is a powerful technique in mapping genes and DNA segments to specific chromosome region. We have been able to release the chromatin fibers from cells arrested at G1 and G2 phases using different drugs and a simple alkaline lysis procedure. We have also demonstrated specific hybridization of fluorescence-labeled probes to single-copy genomic DNA sequences on the free chromatins. Fluorescence in situ hybridization signals have been detected for sequences separated as close as 21 kilobase pairs and as far as 350 kilobase pairs, with excellent correspondence between the observed and expected distances. The resolution of this technique should approach 10 kilobase pairs and its coverage should span millions of base pairs. Therefore, free chromatin mapping can be generally used to study the structure and organization of mammalian genomes.
Persistent Identifierhttp://hdl.handle.net/10722/44255
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHeng, HHQen_HK
dc.contributor.authorSquire, Jen_HK
dc.contributor.authorTsui, LCen_HK
dc.date.accessioned2007-09-12T03:50:00Z-
dc.date.available2007-09-12T03:50:00Z-
dc.date.issued1992en_HK
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America, 1992, v. 89 n. 20, p. 9509-9513en_HK
dc.identifier.issn0027-8424en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44255-
dc.description.abstractFluorescence in situ hybridization to metaphase chromosomes or chromatin fibers in interphase nuclei is a powerful technique in mapping genes and DNA segments to specific chromosome region. We have been able to release the chromatin fibers from cells arrested at G1 and G2 phases using different drugs and a simple alkaline lysis procedure. We have also demonstrated specific hybridization of fluorescence-labeled probes to single-copy genomic DNA sequences on the free chromatins. Fluorescence in situ hybridization signals have been detected for sequences separated as close as 21 kilobase pairs and as far as 350 kilobase pairs, with excellent correspondence between the observed and expected distances. The resolution of this technique should approach 10 kilobase pairs and its coverage should span millions of base pairs. Therefore, free chromatin mapping can be generally used to study the structure and organization of mammalian genomes.en_HK
dc.languageengen_HK
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_HK
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_HK
dc.subject.meshAmsacrine - pharmacologyen_HK
dc.subject.meshChromatin - ultrastructureen_HK
dc.subject.meshIn situ hybridization, fluorescence - methodsen_HK
dc.subject.meshChromosomes, human, pair 7en_HK
dc.subject.meshChromosome mapping - methodsen_HK
dc.titleHigh-resolution mapping of mammalian genes by in situ hybridization to free chromatinen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0027-8424&volume=89&issue=20&spage=9509&epage=9513&date=1992&atitle=High+resolution+mapping+of+mammalian+genes+by+in+situ+hybridization+to+free+chromatinen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1073/pnas.89.20.9509en_HK
dc.identifier.pmid1384055-
dc.identifier.pmcidPMC50161-
dc.identifier.scopuseid_2-s2.0-0026667606en_HK
dc.identifier.volume89en_HK
dc.identifier.issue20en_HK
dc.identifier.spage9509en_HK
dc.identifier.epage9513en_HK
dc.identifier.isiWOS:A1992JT97700030-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHeng, HHQ=7005338076en_HK
dc.identifier.scopusauthoridSquire, J=7102094488en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK

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