File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Temporal regulation of six crystallin transcripts during mouse lens development

TitleTemporal regulation of six crystallin transcripts during mouse lens development
Authors
KeywordsGene-expression
Lens development
Murine γ-crystallins
Polymerase chain reaction
Issue Date1992
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexer
Citation
Experimental Eye Research, 1992, v. 54 n. 5, p. 785-795 How to Cite?
AbstractUsing the polymerase chain reaction (PCR) and RNA blot analysis. we have examined the differential expression patterns of the γ-crystallins during lens development. Since only four of these genes had been previously characterized, the cDNAs for the remaining two genes. γC and γF, were isolated and sequenced. The steady-state mRNA profiles were then determined by RNA blot analysis of samples from embryonic stages to 180 days after birth, with gene-specific probes for γA, γB, γC, and γD, and a common probe for γE and γF. Due to the paucity of mismatches between the γE and γF-crystallin genes, the PCR technique was exploited to determine their relative abundance. The data showed that while all six γ-crystallin genes were expressed in the embryonic lens, they were differentially regulated during development. At early stages, the levels of γB and γC mRNAs were found to be relatively low in comparison to those for γA, γD, γE and γF. After 30-40 days however, the levels of γA, γE, and γF mRNAs declined rapidly, and the γB, γC and γD transcripts became the major γ-crystallin mRNA species. The utility of the PCR technique in studying the relative abundance of steady-state γ-crystallin mRNAs was also investigated.
Persistent Identifierhttp://hdl.handle.net/10722/44253
ISSN
2015 Impact Factor: 2.998
2015 SCImago Journal Rankings: 1.218
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGoring, DRen_HK
dc.contributor.authorBreitman, MLen_HK
dc.contributor.authorTsui, LCen_HK
dc.date.accessioned2007-09-12T03:49:57Z-
dc.date.available2007-09-12T03:49:57Z-
dc.date.issued1992en_HK
dc.identifier.citationExperimental Eye Research, 1992, v. 54 n. 5, p. 785-795en_HK
dc.identifier.issn0014-4835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44253-
dc.description.abstractUsing the polymerase chain reaction (PCR) and RNA blot analysis. we have examined the differential expression patterns of the γ-crystallins during lens development. Since only four of these genes had been previously characterized, the cDNAs for the remaining two genes. γC and γF, were isolated and sequenced. The steady-state mRNA profiles were then determined by RNA blot analysis of samples from embryonic stages to 180 days after birth, with gene-specific probes for γA, γB, γC, and γD, and a common probe for γE and γF. Due to the paucity of mismatches between the γE and γF-crystallin genes, the PCR technique was exploited to determine their relative abundance. The data showed that while all six γ-crystallin genes were expressed in the embryonic lens, they were differentially regulated during development. At early stages, the levels of γB and γC mRNAs were found to be relatively low in comparison to those for γA, γD, γE and γF. After 30-40 days however, the levels of γA, γE, and γF mRNAs declined rapidly, and the γB, γC and γD transcripts became the major γ-crystallin mRNA species. The utility of the PCR technique in studying the relative abundance of steady-state γ-crystallin mRNAs was also investigated.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexeren_HK
dc.relation.ispartofExperimental Eye Researchen_HK
dc.subjectGene-expressionen_HK
dc.subjectLens developmenten_HK
dc.subjectMurine γ-crystallinsen_HK
dc.subjectPolymerase chain reactionen_HK
dc.subject.meshCrystallins - geneticsen_HK
dc.subject.meshGene expression regulation - physiologyen_HK
dc.subject.meshImmunoblottingen_HK
dc.subject.meshLens, crystalline - growth & development - metabolismen_HK
dc.subject.meshPolymerase chain reactionen_HK
dc.titleTemporal regulation of six crystallin transcripts during mouse lens developmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-4835&volume=54&issue=5&spage=785&epage=795&date=1992&atitle=Temporal+regulation+of+six+crystallin+transcripts+during+mouse+lens+developmenten_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1016/0014-4835(92)90034-Pen_HK
dc.identifier.pmid1623964-
dc.identifier.scopuseid_2-s2.0-0026777207en_HK
dc.identifier.volume54en_HK
dc.identifier.issue5en_HK
dc.identifier.spage785en_HK
dc.identifier.epage795en_HK
dc.identifier.isiWOS:A1992HY28900016-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridGoring, DR=7005468517en_HK
dc.identifier.scopusauthoridBreitman, ML=7005448008en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats