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Article: Characterization of the promoter region of the cystic fibrosis transmembrane conductance regulator gene

TitleCharacterization of the promoter region of the cystic fibrosis transmembrane conductance regulator gene
Authors
Issue Date1991
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1991, v. 266 n. 36, p. 24471-24476 How to Cite?
Abstract
To identify the transcription regulatory elements of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, DNA fragments located in the 5'-upstream region were fused with the bacterial chloramphenicol acetyltransferase (CAT) reporter gene and transfected into various cell lines to test for promoter activity. The results of these studies suggested that there were at least two positive and one negative cis-acting elements involved in CFTR transcription initiation. One of them was a proximal, positive element delimited by the 5' deletion constructs -226 base parts upstream of the transcription start site. This minimal promoter sequence (-226 to +98) alone seemed to be sufficient to direct cell-specific CAT expression. The sequences immediately upstream of -277, on the other hand, appeared to contain a negative regulatory element; inclusion of this sequence with the proximal element (e.g. a construct containing sequences -345 to +98) rendered the CFTR promoter inactive. This negative regulatory element could also suppress the activity of a heterologous promoter. In addition, the DNA transfection study suggested the existence of another positive regulatory element outside the CFTR promoter region examined, as the inability of this region (e.g. -658 to +98) to function in a CAT assay could be overcome by the presence of a viral enhancer element.
Persistent Identifierhttp://hdl.handle.net/10722/44249
ISSN
2013 Impact Factor: 4.600
Other Identifiers
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChou, JLen_HK
dc.contributor.authorRozmahel, Ren_HK
dc.contributor.authorTsui, LCen_HK
dc.date.accessioned2007-09-12T03:49:52Z-
dc.date.available2007-09-12T03:49:52Z-
dc.date.issued1991en_HK
dc.identifierhttp://www.jbc.org/cgi/reprint/266/36/24471.pdfen_HK
dc.identifier.citationJournal Of Biological Chemistry, 1991, v. 266 n. 36, p. 24471-24476en_HK
dc.identifier.issn0021-9258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44249-
dc.description.abstractTo identify the transcription regulatory elements of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, DNA fragments located in the 5'-upstream region were fused with the bacterial chloramphenicol acetyltransferase (CAT) reporter gene and transfected into various cell lines to test for promoter activity. The results of these studies suggested that there were at least two positive and one negative cis-acting elements involved in CFTR transcription initiation. One of them was a proximal, positive element delimited by the 5' deletion constructs -226 base parts upstream of the transcription start site. This minimal promoter sequence (-226 to +98) alone seemed to be sufficient to direct cell-specific CAT expression. The sequences immediately upstream of -277, on the other hand, appeared to contain a negative regulatory element; inclusion of this sequence with the proximal element (e.g. a construct containing sequences -345 to +98) rendered the CFTR promoter inactive. This negative regulatory element could also suppress the activity of a heterologous promoter. In addition, the DNA transfection study suggested the existence of another positive regulatory element outside the CFTR promoter region examined, as the inability of this region (e.g. -658 to +98) to function in a CAT assay could be overcome by the presence of a viral enhancer element.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_HK
dc.relation.ispartofJournal of Biological Chemistryen_HK
dc.rightsJournal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshChloramphenicol o-acetyltransferase - genetics - metabolismen_HK
dc.subject.meshCystic fibrosis - geneticsen_HK
dc.subject.meshMembrane proteins - geneticsen_HK
dc.subject.meshPromoter regions (genetics)en_HK
dc.subject.meshNucleic acid hybridizationen_HK
dc.titleCharacterization of the promoter region of the cystic fibrosis transmembrane conductance regulator geneen_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.pmid1722205en_HK
dc.identifier.scopuseid_2-s2.0-0026327058en_HK
dc.identifier.volume266en_HK
dc.identifier.issue36en_HK
dc.identifier.spage24471en_HK
dc.identifier.epage24476en_HK
dc.identifier.isiWOS:A1991GW84500037-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChou, JL=24443879300en_HK
dc.identifier.scopusauthoridRozmahel, R=6701510561en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK

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