Article: Characterization of the promoter region of the cystic fibrosis transmembrane conductance regulator gene

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TitleCharacterization of the promoter region of the cystic fibrosis transmembrane conductance regulator gene
AuthorsChou, JL1
Rozmahel, R1
Tsui, LC1
Issue Date1991
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
CitationJournal Of Biological Chemistry, 1991, v. 266 n. 36, p. 24471-24476 [How to Cite?]
AbstractTo identify the transcription regulatory elements of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, DNA fragments located in the 5'-upstream region were fused with the bacterial chloramphenicol acetyltransferase (CAT) reporter gene and transfected into various cell lines to test for promoter activity. The results of these studies suggested that there were at least two positive and one negative cis-acting elements involved in CFTR transcription initiation. One of them was a proximal, positive element delimited by the 5' deletion constructs -226 base parts upstream of the transcription start site. This minimal promoter sequence (-226 to +98) alone seemed to be sufficient to direct cell-specific CAT expression. The sequences immediately upstream of -277, on the other hand, appeared to contain a negative regulatory element; inclusion of this sequence with the proximal element (e.g. a construct containing sequences -345 to +98) rendered the CFTR promoter inactive. This negative regulatory element could also suppress the activity of a heterologous promoter. In addition, the DNA transfection study suggested the existence of another positive regulatory element outside the CFTR promoter region examined, as the inability of this region (e.g. -658 to +98) to function in a CAT assay could be overcome by the presence of a viral enhancer element.
ISSN0021-9258
2011 Impact Factor: 4.773
2011 SCImago Journal Rankings: 0.793
Other Identifiershttp://www.jbc.org/cgi/reprint/266/36/24471.pdf
ISI Accession Number IDWOS:A1991GW84500037
DC Field
Value
dc.contributor.authorChou, JL
dc.contributor.authorRozmahel, R
dc.contributor.authorTsui, LC
dc.date.accessioned2007-09-12T03:49:52Z
dc.date.available2007-09-12T03:49:52Z
dc.date.issued1991
dc.description.abstractTo identify the transcription regulatory elements of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, DNA fragments located in the 5'-upstream region were fused with the bacterial chloramphenicol acetyltransferase (CAT) reporter gene and transfected into various cell lines to test for promoter activity. The results of these studies suggested that there were at least two positive and one negative cis-acting elements involved in CFTR transcription initiation. One of them was a proximal, positive element delimited by the 5' deletion constructs -226 base parts upstream of the transcription start site. This minimal promoter sequence (-226 to +98) alone seemed to be sufficient to direct cell-specific CAT expression. The sequences immediately upstream of -277, on the other hand, appeared to contain a negative regulatory element; inclusion of this sequence with the proximal element (e.g. a construct containing sequences -345 to +98) rendered the CFTR promoter inactive. This negative regulatory element could also suppress the activity of a heterologous promoter. In addition, the DNA transfection study suggested the existence of another positive regulatory element outside the CFTR promoter region examined, as the inability of this region (e.g. -658 to +98) to function in a CAT assay could be overcome by the presence of a viral enhancer element.
dc.description.naturepublished_or_final_version
dc.identifier.citationJournal Of Biological Chemistry, 1991, v. 266 n. 36, p. 24471-24476 [How to Cite?]
dc.identifier.epage24476
dc.identifier.isiWOS:A1991GW84500037
dc.identifier.issn0021-9258
2011 Impact Factor: 4.773
2011 SCImago Journal Rankings: 0.793
dc.identifier.issue36
dc.identifierhttp://www.jbc.org/cgi/reprint/266/36/24471.pdf
dc.identifier.pmid1722205
dc.identifier.scopuseid_2-s2.0-0026327058
dc.identifier.spage24471
dc.identifier.urihttp://hdl.handle.net/10722/44249
dc.identifier.volume266
dc.languageeng
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Biological Chemistry
dc.rightsJournal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc.
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.subject.meshChloramphenicol o-acetyltransferase - genetics - metabolism
dc.subject.meshCystic fibrosis - genetics
dc.subject.meshMembrane proteins - genetics
dc.subject.meshPromoter regions (genetics)
dc.subject.meshNucleic acid hybridization
dc.titleCharacterization of the promoter region of the cystic fibrosis transmembrane conductance regulator gene
dc.typeArticle
Author Affiliations
  1. Hospital for Sick Children, Toronto