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Article: Characterization of the promoter region of the cystic fibrosis transmembrane conductance regulator gene
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TitleCharacterization of the promoter region of the cystic fibrosis transmembrane conductance regulator gene
 
AuthorsChou, JL1
Rozmahel, R1
Tsui, LC1
 
Issue Date1991
 
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
 
CitationJournal Of Biological Chemistry, 1991, v. 266 n. 36, p. 24471-24476 [How to Cite?]
 
AbstractTo identify the transcription regulatory elements of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, DNA fragments located in the 5'-upstream region were fused with the bacterial chloramphenicol acetyltransferase (CAT) reporter gene and transfected into various cell lines to test for promoter activity. The results of these studies suggested that there were at least two positive and one negative cis-acting elements involved in CFTR transcription initiation. One of them was a proximal, positive element delimited by the 5' deletion constructs -226 base parts upstream of the transcription start site. This minimal promoter sequence (-226 to +98) alone seemed to be sufficient to direct cell-specific CAT expression. The sequences immediately upstream of -277, on the other hand, appeared to contain a negative regulatory element; inclusion of this sequence with the proximal element (e.g. a construct containing sequences -345 to +98) rendered the CFTR promoter inactive. This negative regulatory element could also suppress the activity of a heterologous promoter. In addition, the DNA transfection study suggested the existence of another positive regulatory element outside the CFTR promoter region examined, as the inability of this region (e.g. -658 to +98) to function in a CAT assay could be overcome by the presence of a viral enhancer element.
 
ISSN0021-9258
2012 Impact Factor: 4.651
2012 SCImago Journal Rankings: 2.723
 
Other Identifiershttp://www.jbc.org/cgi/reprint/266/36/24471.pdf
 
ISI Accession Number IDWOS:A1991GW84500037
 
DC FieldValue
dc.contributor.authorChou, JL
 
dc.contributor.authorRozmahel, R
 
dc.contributor.authorTsui, LC
 
dc.date.accessioned2007-09-12T03:49:52Z
 
dc.date.available2007-09-12T03:49:52Z
 
dc.date.issued1991
 
dc.description.abstractTo identify the transcription regulatory elements of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, DNA fragments located in the 5'-upstream region were fused with the bacterial chloramphenicol acetyltransferase (CAT) reporter gene and transfected into various cell lines to test for promoter activity. The results of these studies suggested that there were at least two positive and one negative cis-acting elements involved in CFTR transcription initiation. One of them was a proximal, positive element delimited by the 5' deletion constructs -226 base parts upstream of the transcription start site. This minimal promoter sequence (-226 to +98) alone seemed to be sufficient to direct cell-specific CAT expression. The sequences immediately upstream of -277, on the other hand, appeared to contain a negative regulatory element; inclusion of this sequence with the proximal element (e.g. a construct containing sequences -345 to +98) rendered the CFTR promoter inactive. This negative regulatory element could also suppress the activity of a heterologous promoter. In addition, the DNA transfection study suggested the existence of another positive regulatory element outside the CFTR promoter region examined, as the inability of this region (e.g. -658 to +98) to function in a CAT assay could be overcome by the presence of a viral enhancer element.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationJournal Of Biological Chemistry, 1991, v. 266 n. 36, p. 24471-24476 [How to Cite?]
 
dc.identifier.epage24476
 
dc.identifier.isiWOS:A1991GW84500037
 
dc.identifier.issn0021-9258
2012 Impact Factor: 4.651
2012 SCImago Journal Rankings: 2.723
 
dc.identifier.issue36
 
dc.identifierhttp://www.jbc.org/cgi/reprint/266/36/24471.pdf
 
dc.identifier.pmid1722205
 
dc.identifier.scopuseid_2-s2.0-0026327058
 
dc.identifier.spage24471
 
dc.identifier.urihttp://hdl.handle.net/10722/44249
 
dc.identifier.volume266
 
dc.languageeng
 
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Biological Chemistry
 
dc.rightsJournal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshChloramphenicol o-acetyltransferase - genetics - metabolism
 
dc.subject.meshCystic fibrosis - genetics
 
dc.subject.meshMembrane proteins - genetics
 
dc.subject.meshPromoter regions (genetics)
 
dc.subject.meshNucleic acid hybridization
 
dc.titleCharacterization of the promoter region of the cystic fibrosis transmembrane conductance regulator gene
 
dc.typeArticle
 
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<contributor.author>Rozmahel, R</contributor.author>
<contributor.author>Tsui, LC</contributor.author>
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<date.available>2007-09-12T03:49:52Z</date.available>
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<description.abstract>To identify the transcription regulatory elements of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, DNA fragments located in the 5&apos;-upstream region were fused with the bacterial chloramphenicol acetyltransferase (CAT) reporter gene and transfected into various cell lines to test for promoter activity. The results of these studies suggested that there were at least two positive and one negative cis-acting elements involved in CFTR transcription initiation. One of them was a proximal, positive element delimited by the 5&apos; deletion constructs -226 base parts upstream of the transcription start site. This minimal promoter sequence (-226 to +98) alone seemed to be sufficient to direct cell-specific CAT expression. The sequences immediately upstream of -277, on the other hand, appeared to contain a negative regulatory element; inclusion of this sequence with the proximal element (e.g. a construct containing sequences -345 to +98) rendered the CFTR promoter inactive. This negative regulatory element could also suppress the activity of a heterologous promoter. In addition, the DNA transfection study suggested the existence of another positive regulatory element outside the CFTR promoter region examined, as the inability of this region (e.g. -658 to +98) to function in a CAT assay could be overcome by the presence of a viral enhancer element.</description.abstract>
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<subject.mesh>Chloramphenicol o-acetyltransferase - genetics - metabolism</subject.mesh>
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Author Affiliations
  1. Hospital for Sick Children University of Toronto