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Article: cAMP-inducible chloride conductance in mouse fibroblast lines stably expressing the human cystic fibrosis transmembrane conductance regulator

TitlecAMP-inducible chloride conductance in mouse fibroblast lines stably expressing the human cystic fibrosis transmembrane conductance regulator
Authors
KeywordscAMP regulation
chloride channel
full-length cDNA
Issue Date1991
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings Of The National Academy Of Sciences Of The United States Of America, 1991, v. 88 n. 17, p. 7500-7504 How to Cite?
AbstractA cAMP-inducible chloride permeability has been detected in mouse fibroblast (L cell) lines upon stable integration of a full-length cDNA encoding the human cystic fibrosis transmembrane conductance regulator (CFTR). As indicated by a Cl--indicator dye, the Cl- permeability of the plasma membrane increases by 10- to 30-fold within 2 min after treatment of the cells with forskolin, an activator of adenylyl cyclase. The properties of the conductance are similar to those described in secretory epithelial cells; the whole-cell current-voltage relationship is linear and there is no evidence of voltage-dependent inactivation or activation. In contrast, this cAMP-dependent Cl- flux is undetectable in the untransfected cells or cells harboring defective cDNA constructs, including one with a phenylalanine deletion at amino acid position 508 (ΔF508), the most common mutation causing cystic fibrosis. These observations are consistent with the hypothesis that the CFTR is a cAMP-dependent Cl- channel. The availability of a heterologous (nonepithelial) cell type expressing the CFTR offers an excellent system to understand the basic mechanisms underlying this CFTR-associated ion permeability and to study the structure and function of the CFTR.
Persistent Identifierhttp://hdl.handle.net/10722/44246
ISSN
2014 Impact Factor: 9.674
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRommens, JMen_HK
dc.contributor.authorDho, Sen_HK
dc.contributor.authorBear, CEen_HK
dc.contributor.authorKartner, Nen_HK
dc.contributor.authorKennedy, Den_HK
dc.contributor.authorRiordan, JRen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorFoskett, JKen_HK
dc.date.accessioned2007-09-12T03:49:48Z-
dc.date.available2007-09-12T03:49:48Z-
dc.date.issued1991en_HK
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America, 1991, v. 88 n. 17, p. 7500-7504en_HK
dc.identifier.issn0027-8424en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44246-
dc.description.abstractA cAMP-inducible chloride permeability has been detected in mouse fibroblast (L cell) lines upon stable integration of a full-length cDNA encoding the human cystic fibrosis transmembrane conductance regulator (CFTR). As indicated by a Cl--indicator dye, the Cl- permeability of the plasma membrane increases by 10- to 30-fold within 2 min after treatment of the cells with forskolin, an activator of adenylyl cyclase. The properties of the conductance are similar to those described in secretory epithelial cells; the whole-cell current-voltage relationship is linear and there is no evidence of voltage-dependent inactivation or activation. In contrast, this cAMP-dependent Cl- flux is undetectable in the untransfected cells or cells harboring defective cDNA constructs, including one with a phenylalanine deletion at amino acid position 508 (ΔF508), the most common mutation causing cystic fibrosis. These observations are consistent with the hypothesis that the CFTR is a cAMP-dependent Cl- channel. The availability of a heterologous (nonepithelial) cell type expressing the CFTR offers an excellent system to understand the basic mechanisms underlying this CFTR-associated ion permeability and to study the structure and function of the CFTR.en_HK
dc.languageengen_HK
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_HK
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_HK
dc.subjectcAMP regulationen_HK
dc.subjectchloride channelen_HK
dc.subjectfull-length cDNAen_HK
dc.subject.meshChlorides - metabolismen_HK
dc.subject.meshFull-length cdnaen_HK
dc.subject.meshChloride channelen_HK
dc.subject.meshCamp regulationen_HK
dc.subject.meshCystic fibrosis - genetics - physiopathologyen_HK
dc.titlecAMP-inducible chloride conductance in mouse fibroblast lines stably expressing the human cystic fibrosis transmembrane conductance regulatoren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0027-8424&volume=88&issue=17&spage=7500&epage=7504&date=1991&atitle=Cyclic-AMP-inducible+chloride+conductance+in+mouse+fibroblast+lines+stably+expressing+human+cystic+fibrosis+transmembrane+conductance+regulatoren_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_OA_fulltexten_HK
dc.identifier.pmid1715567en_HK
dc.identifier.pmcidPMC52328-
dc.identifier.scopuseid_2-s2.0-0025807911en_HK
dc.identifier.volume88en_HK
dc.identifier.issue17en_HK
dc.identifier.spage7500en_HK
dc.identifier.epage7504en_HK
dc.identifier.isiWOS:A1991GC99200010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridRommens, JM=7006884140en_HK
dc.identifier.scopusauthoridDho, S=6603797159en_HK
dc.identifier.scopusauthoridBear, CE=7006718679en_HK
dc.identifier.scopusauthoridKartner, N=6701620107en_HK
dc.identifier.scopusauthoridKennedy, D=7403112289en_HK
dc.identifier.scopusauthoridRiordan, JR=7202229758en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridFoskett, JK=7005723620en_HK

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