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Article: Molecular cloning and sequence analysis of the murine cDNA for the cystic fibrosis transmembrane conductance regulator

TitleMolecular cloning and sequence analysis of the murine cDNA for the cystic fibrosis transmembrane conductance regulator
Authors
Issue Date1991
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygeno
Citation
Genomics, 1991, v. 10 n. 3, p. 547-550 How to Cite?
Abstract
We have cloned the mouse homolog of the human cystic fibrosis transmembrane conductance regulator (CFTR) using clones isolated from a mouse lung cDNA library and using amplification of cDNA to isolate specific regions. The cDNA was 6304 bp in length and encoded a polypeptide of 1476 amino acids. Comparison of the deduced amino acid sequence showed that the mouse protein has high homology to the human protein; overall identity was 78.3%. The amino acid identity was high for both transmembrane domains (first transmembrane domain, 86.7%; second transmembrane domain, 81.1%) and for both ATP-binding folds (first ATP-binding fold, 80.5%; second ATP-binding fold, 83.9%), suggesting the functional importance of these regions. On the other hand, the R domain was less well conserved (68.9% identity). All of the published missense mutation sites and the site of the common ΔF508 mutation were conserved between human and mouse.
Persistent Identifierhttp://hdl.handle.net/10722/44245
ISSN
2013 Impact Factor: 2.793
ISI Accession Number ID

 

Author Affiliations
  1. Baylor College of Medicine
DC FieldValueLanguage
dc.contributor.authorYorifuji, Ten_HK
dc.contributor.authorLemna, WKen_HK
dc.contributor.authorBallard, CFen_HK
dc.contributor.authorRosenbloom, CLen_HK
dc.contributor.authorRozmahel, Ren_HK
dc.contributor.authorPlavsic, Nen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorBeaudet, ALen_HK
dc.date.accessioned2007-09-12T03:49:47Z-
dc.date.available2007-09-12T03:49:47Z-
dc.date.issued1991en_HK
dc.identifier.citationGenomics, 1991, v. 10 n. 3, p. 547-550en_HK
dc.identifier.issn0888-7543en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44245-
dc.description.abstractWe have cloned the mouse homolog of the human cystic fibrosis transmembrane conductance regulator (CFTR) using clones isolated from a mouse lung cDNA library and using amplification of cDNA to isolate specific regions. The cDNA was 6304 bp in length and encoded a polypeptide of 1476 amino acids. Comparison of the deduced amino acid sequence showed that the mouse protein has high homology to the human protein; overall identity was 78.3%. The amino acid identity was high for both transmembrane domains (first transmembrane domain, 86.7%; second transmembrane domain, 81.1%) and for both ATP-binding folds (first ATP-binding fold, 80.5%; second ATP-binding fold, 83.9%), suggesting the functional importance of these regions. On the other hand, the R domain was less well conserved (68.9% identity). All of the published missense mutation sites and the site of the common ΔF508 mutation were conserved between human and mouse.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygenoen_HK
dc.relation.ispartofGenomicsen_HK
dc.subject.meshCystic fibrosis transmembrane conductance regulatoren_HK
dc.subject.meshAmino acid sequenceen_HK
dc.subject.meshDna - geneticsen_HK
dc.subject.meshMembrane proteins - geneticsen_HK
dc.subject.meshMice - geneticsen_HK
dc.titleMolecular cloning and sequence analysis of the murine cDNA for the cystic fibrosis transmembrane conductance regulatoren_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.natureabstracten_HK
dc.identifier.doi10.1016/0888-7543(91)90434-Gen_HK
dc.identifier.pmid1716243en_HK
dc.identifier.scopuseid_2-s2.0-0025789705en_HK
dc.identifier.volume10en_HK
dc.identifier.issue3en_HK
dc.identifier.spage547en_HK
dc.identifier.epage550en_HK
dc.identifier.isiWOS:A1991FQ64000005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYorifuji, T=16154876500en_HK
dc.identifier.scopusauthoridLemna, WK=6506413347en_HK
dc.identifier.scopusauthoridBallard, CF=7101938620en_HK
dc.identifier.scopusauthoridRosenbloom, CL=36807850700en_HK
dc.identifier.scopusauthoridRozmahel, R=6701510561en_HK
dc.identifier.scopusauthoridPlavsic, N=8314412600en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridBeaudet, AL=7102581677en_HK

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