File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Molecular cloning and sequence analysis of the murine cDNA for the cystic fibrosis transmembrane conductance regulator
  • Basic View
  • Metadata View
  • XML View
TitleMolecular cloning and sequence analysis of the murine cDNA for the cystic fibrosis transmembrane conductance regulator
 
AuthorsYorifuji, T1
Lemna, WK1
Ballard, CF1
Rosenbloom, CL1
Rozmahel, R1
Plavsic, N1
Tsui, LC1
Beaudet, AL1
 
Issue Date1991
 
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygeno
 
CitationGenomics, 1991, v. 10 n. 3, p. 547-550 [How to Cite?]
DOI: http://dx.doi.org/10.1016/0888-7543(91)90434-G
 
AbstractWe have cloned the mouse homolog of the human cystic fibrosis transmembrane conductance regulator (CFTR) using clones isolated from a mouse lung cDNA library and using amplification of cDNA to isolate specific regions. The cDNA was 6304 bp in length and encoded a polypeptide of 1476 amino acids. Comparison of the deduced amino acid sequence showed that the mouse protein has high homology to the human protein; overall identity was 78.3%. The amino acid identity was high for both transmembrane domains (first transmembrane domain, 86.7%; second transmembrane domain, 81.1%) and for both ATP-binding folds (first ATP-binding fold, 80.5%; second ATP-binding fold, 83.9%), suggesting the functional importance of these regions. On the other hand, the R domain was less well conserved (68.9% identity). All of the published missense mutation sites and the site of the common ΔF508 mutation were conserved between human and mouse.
 
ISSN0888-7543
2012 Impact Factor: 3.01
2012 SCImago Journal Rankings: 1.280
 
DOIhttp://dx.doi.org/10.1016/0888-7543(91)90434-G
 
ISI Accession Number IDWOS:A1991FQ64000005
 
DC FieldValue
dc.contributor.authorYorifuji, T
 
dc.contributor.authorLemna, WK
 
dc.contributor.authorBallard, CF
 
dc.contributor.authorRosenbloom, CL
 
dc.contributor.authorRozmahel, R
 
dc.contributor.authorPlavsic, N
 
dc.contributor.authorTsui, LC
 
dc.contributor.authorBeaudet, AL
 
dc.date.accessioned2007-09-12T03:49:47Z
 
dc.date.available2007-09-12T03:49:47Z
 
dc.date.issued1991
 
dc.description.abstractWe have cloned the mouse homolog of the human cystic fibrosis transmembrane conductance regulator (CFTR) using clones isolated from a mouse lung cDNA library and using amplification of cDNA to isolate specific regions. The cDNA was 6304 bp in length and encoded a polypeptide of 1476 amino acids. Comparison of the deduced amino acid sequence showed that the mouse protein has high homology to the human protein; overall identity was 78.3%. The amino acid identity was high for both transmembrane domains (first transmembrane domain, 86.7%; second transmembrane domain, 81.1%) and for both ATP-binding folds (first ATP-binding fold, 80.5%; second ATP-binding fold, 83.9%), suggesting the functional importance of these regions. On the other hand, the R domain was less well conserved (68.9% identity). All of the published missense mutation sites and the site of the common ΔF508 mutation were conserved between human and mouse.
 
dc.description.natureabstract
 
dc.identifier.citationGenomics, 1991, v. 10 n. 3, p. 547-550 [How to Cite?]
DOI: http://dx.doi.org/10.1016/0888-7543(91)90434-G
 
dc.identifier.doihttp://dx.doi.org/10.1016/0888-7543(91)90434-G
 
dc.identifier.epage550
 
dc.identifier.isiWOS:A1991FQ64000005
 
dc.identifier.issn0888-7543
2012 Impact Factor: 3.01
2012 SCImago Journal Rankings: 1.280
 
dc.identifier.issue3
 
dc.identifier.pmid1716243
 
dc.identifier.scopuseid_2-s2.0-0025789705
 
dc.identifier.spage547
 
dc.identifier.urihttp://hdl.handle.net/10722/44245
 
dc.identifier.volume10
 
dc.languageeng
 
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygeno
 
dc.publisher.placeUnited States
 
dc.relation.ispartofGenomics
 
dc.subject.meshCystic fibrosis transmembrane conductance regulator
 
dc.subject.meshAmino acid sequence
 
dc.subject.meshDna - genetics
 
dc.subject.meshMembrane proteins - genetics
 
dc.subject.meshMice - genetics
 
dc.titleMolecular cloning and sequence analysis of the murine cDNA for the cystic fibrosis transmembrane conductance regulator
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Yorifuji, T</contributor.author>
<contributor.author>Lemna, WK</contributor.author>
<contributor.author>Ballard, CF</contributor.author>
<contributor.author>Rosenbloom, CL</contributor.author>
<contributor.author>Rozmahel, R</contributor.author>
<contributor.author>Plavsic, N</contributor.author>
<contributor.author>Tsui, LC</contributor.author>
<contributor.author>Beaudet, AL</contributor.author>
<date.accessioned>2007-09-12T03:49:47Z</date.accessioned>
<date.available>2007-09-12T03:49:47Z</date.available>
<date.issued>1991</date.issued>
<identifier.citation>Genomics, 1991, v. 10 n. 3, p. 547-550</identifier.citation>
<identifier.issn>0888-7543</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/44245</identifier.uri>
<description.abstract>We have cloned the mouse homolog of the human cystic fibrosis transmembrane conductance regulator (CFTR) using clones isolated from a mouse lung cDNA library and using amplification of cDNA to isolate specific regions. The cDNA was 6304 bp in length and encoded a polypeptide of 1476 amino acids. Comparison of the deduced amino acid sequence showed that the mouse protein has high homology to the human protein; overall identity was 78.3%. The amino acid identity was high for both transmembrane domains (first transmembrane domain, 86.7%; second transmembrane domain, 81.1%) and for both ATP-binding folds (first ATP-binding fold, 80.5%; second ATP-binding fold, 83.9%), suggesting the functional importance of these regions. On the other hand, the R domain was less well conserved (68.9% identity). All of the published missense mutation sites and the site of the common &#916;F508 mutation were conserved between human and mouse.</description.abstract>
<language>eng</language>
<publisher>Academic Press. The Journal&apos;s web site is located at http://www.elsevier.com/locate/ygeno</publisher>
<relation.ispartof>Genomics</relation.ispartof>
<subject.mesh>Cystic fibrosis transmembrane conductance regulator</subject.mesh>
<subject.mesh>Amino acid sequence</subject.mesh>
<subject.mesh>Dna - genetics</subject.mesh>
<subject.mesh>Membrane proteins - genetics</subject.mesh>
<subject.mesh>Mice - genetics</subject.mesh>
<title>Molecular cloning and sequence analysis of the murine cDNA for the cystic fibrosis transmembrane conductance regulator</title>
<type>Article</type>
<description.nature>abstract</description.nature>
<identifier.doi>10.1016/0888-7543(91)90434-G</identifier.doi>
<identifier.pmid>1716243</identifier.pmid>
<identifier.scopus>eid_2-s2.0-0025789705</identifier.scopus>
<identifier.volume>10</identifier.volume>
<identifier.issue>3</identifier.issue>
<identifier.spage>547</identifier.spage>
<identifier.epage>550</identifier.epage>
<identifier.isi>WOS:A1991FQ64000005</identifier.isi>
<publisher.place>United States</publisher.place>
</item>
Author Affiliations
  1. Baylor College of Medicine