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Article: Expression of the cystic fibrosis gene in non-epithelial invertebrate cells produces a regulated anion conductance

TitleExpression of the cystic fibrosis gene in non-epithelial invertebrate cells produces a regulated anion conductance
Authors
Issue Date1991
PublisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/cell
Citation
Cell, 1991, v. 64 n. 4, p. 681-691 How to Cite?
AbstractThe nature of involvement of the cystic fibrosis gene product (CFTR) in epithelial anion transport is not yet understood. We have expressed CFTR in Sf9 insect cells using the baculovirus expression vector system. Reactivity with antibodies against 12 different epitopes spanning the entire sequence suggested that the complete polypeptide chain was synthesized. Immunogold labeling showed localization to both cell-surface and intracellular membranes. Concomitant with CFTR expression, these cells exhibited a new cAMP-stimulated anion permeability. This conductance, monitored both by radioiodide efflux and patch clamping, strongly resembled that present in several CFTR-expressing human epithelial cells. These findings demonstrate that CFTR can function in heterologous nonepithelial cells and lend support to the possibility that CFTR may itself be a regulated anion channel.
Persistent Identifierhttp://hdl.handle.net/10722/44244
ISSN
2015 Impact Factor: 28.71
2015 SCImago Journal Rankings: 28.188
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKartner, Nen_HK
dc.contributor.authorHanrahan, JWen_HK
dc.contributor.authorJensen, TJen_HK
dc.contributor.authorNaismith, ALen_HK
dc.contributor.authorShizhang, Sen_HK
dc.contributor.authorAckerley, CAen_HK
dc.contributor.authorReyes, EFen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorRommens, JMen_HK
dc.contributor.authorBear, CEen_HK
dc.contributor.authorRiordan, JRen_HK
dc.date.accessioned2007-09-12T03:49:46Z-
dc.date.available2007-09-12T03:49:46Z-
dc.date.issued1991en_HK
dc.identifier.citationCell, 1991, v. 64 n. 4, p. 681-691en_HK
dc.identifier.issn0092-8674en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44244-
dc.description.abstractThe nature of involvement of the cystic fibrosis gene product (CFTR) in epithelial anion transport is not yet understood. We have expressed CFTR in Sf9 insect cells using the baculovirus expression vector system. Reactivity with antibodies against 12 different epitopes spanning the entire sequence suggested that the complete polypeptide chain was synthesized. Immunogold labeling showed localization to both cell-surface and intracellular membranes. Concomitant with CFTR expression, these cells exhibited a new cAMP-stimulated anion permeability. This conductance, monitored both by radioiodide efflux and patch clamping, strongly resembled that present in several CFTR-expressing human epithelial cells. These findings demonstrate that CFTR can function in heterologous nonepithelial cells and lend support to the possibility that CFTR may itself be a regulated anion channel.en_HK
dc.languageengen_HK
dc.publisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/cellen_HK
dc.relation.ispartofCellen_HK
dc.rightsThis paper has been submitted to Cancer Cell for considerationen_HK
dc.subject.meshBaculoviridae - geneticsen_HK
dc.subject.meshCell membrane - physiology - ultrastructureen_HK
dc.subject.meshCyclic amp - pharmacologyen_HK
dc.subject.meshCystic fibrosis - geneticsen_HK
dc.subject.meshIon channels - genetics - physiologyen_HK
dc.titleExpression of the cystic fibrosis gene in non-epithelial invertebrate cells produces a regulated anion conductanceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0092-8674&volume=64&issue=4&spage=681&epage=691&date=1991&atitle=Expression+of+the+cystic+fibrosis+gene+in+non-epithelial+invertebrate+cells+produces+a+regulated+anion+conductanceen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1016/0092-8674(91)90498-N-
dc.identifier.pmid1705179-
dc.identifier.scopuseid_2-s2.0-0026073070en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0026073070&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume64en_HK
dc.identifier.issue4en_HK
dc.identifier.spage681en_HK
dc.identifier.epage691en_HK
dc.identifier.isiWOS:A1991EZ47800004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKartner, N=6701620107en_HK
dc.identifier.scopusauthoridHanrahan, JW=7103386837en_HK
dc.identifier.scopusauthoridJensen, TJ=7401539543en_HK
dc.identifier.scopusauthoridNaismith, AL=6602332581en_HK
dc.identifier.scopusauthoridShizhang, S=6504433612en_HK
dc.identifier.scopusauthoridAckerley, CA=7006092057en_HK
dc.identifier.scopusauthoridReyes, EF=7102192268en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridRommens, JM=7006884140en_HK
dc.identifier.scopusauthoridBear, CE=7006718679en_HK
dc.identifier.scopusauthoridRiordan, JR=7202229758en_HK

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