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Article: Correction of the cystic fibrosis defect in vitro by retrovirus-mediated gene transfer

TitleCorrection of the cystic fibrosis defect in vitro by retrovirus-mediated gene transfer
Authors
Issue Date1990
PublisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/cell
Citation
Cell, 1990, v. 62 n. 6, p. 1227-1233 How to Cite?
AbstractWe have used retrovirus-mediated gene transfer to demonstrate complementation of the cystic fibrosis (CF) defect in vitro. Amphotropic retroviruses were used to transduce a functional cystic fibrosis transmembrane conductance regulator (CFTR) cDNA into CFPAC-1, a pancreatic adenocarcinoma cell line derived from a patient with CF that stably expresses the chloride transport abnormalities characteristic of CF. CFPAC-1 cells were exposed to control virus (PLJ) and CFTR-expressing virus (PLJ-CFTR); viral-transduced clones were isolated and subjected to molecular and physiologic analysis. RNA analysis detected a viral-derived CFTR transcript in all of the PLJ-CFTR clones that contained unrearranged proviral sequences. Agents that increase intracellular cAMP stimulated 125I efflux in PLJ-CFTR clones but not PLJ clones. Whole-cell patch-clamp performed on three responding clones showed that the anion efflux responses were due to cAMP stimulation of Cl conductance. Our findings indicate that expression of the normal CFTR gene confers cAMP-dependent Cl channel regulation on CF epithelial cells.
Persistent Identifierhttp://hdl.handle.net/10722/44240
ISSN
2015 Impact Factor: 28.71
2015 SCImago Journal Rankings: 28.188
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDrumm, MLen_HK
dc.contributor.authorPope, HAen_HK
dc.contributor.authorCliff, WHen_HK
dc.contributor.authorRommens, JMen_HK
dc.contributor.authorMarvin, SAen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorCollins, FSen_HK
dc.contributor.authorFrizzell, RAen_HK
dc.contributor.authorWilson, JMen_HK
dc.date.accessioned2007-09-12T03:49:41Z-
dc.date.available2007-09-12T03:49:41Z-
dc.date.issued1990en_HK
dc.identifier.citationCell, 1990, v. 62 n. 6, p. 1227-1233en_HK
dc.identifier.issn0092-8674en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44240-
dc.description.abstractWe have used retrovirus-mediated gene transfer to demonstrate complementation of the cystic fibrosis (CF) defect in vitro. Amphotropic retroviruses were used to transduce a functional cystic fibrosis transmembrane conductance regulator (CFTR) cDNA into CFPAC-1, a pancreatic adenocarcinoma cell line derived from a patient with CF that stably expresses the chloride transport abnormalities characteristic of CF. CFPAC-1 cells were exposed to control virus (PLJ) and CFTR-expressing virus (PLJ-CFTR); viral-transduced clones were isolated and subjected to molecular and physiologic analysis. RNA analysis detected a viral-derived CFTR transcript in all of the PLJ-CFTR clones that contained unrearranged proviral sequences. Agents that increase intracellular cAMP stimulated 125I efflux in PLJ-CFTR clones but not PLJ clones. Whole-cell patch-clamp performed on three responding clones showed that the anion efflux responses were due to cAMP stimulation of Cl conductance. Our findings indicate that expression of the normal CFTR gene confers cAMP-dependent Cl channel regulation on CF epithelial cells.en_HK
dc.languageengen_HK
dc.publisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/cellen_HK
dc.relation.ispartofCellen_HK
dc.rightsThis paper has been submitted to Cancer Cell for considerationen_HK
dc.subject.meshAdenocarcinoma - genetics - physiopathologyen_HK
dc.subject.meshChloride channelsen_HK
dc.subject.meshCyclic amp - metabolismen_HK
dc.subject.meshCystic fibrosis - geneticsen_HK
dc.subject.meshIon channels - physiologyen_HK
dc.titleCorrection of the cystic fibrosis defect in vitro by retrovirus-mediated gene transferen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0092-8674&volume=62&issue=6&spage=1227&epage=1233&date=1990&atitle=Correction+of+the+cystic+fibrosis+defect+in+vitro+by+retrovirus-mediated+gene+transferen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1016/0092-8674(90)90398-Xen_HK
dc.identifier.pmid1698126en_HK
dc.identifier.scopuseid_2-s2.0-0025094612en_HK
dc.identifier.volume62en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1227en_HK
dc.identifier.epage1233en_HK
dc.identifier.isiWOS:A1990EA11700019-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDrumm, ML=7006422406en_HK
dc.identifier.scopusauthoridPope, HA=36794690500en_HK
dc.identifier.scopusauthoridCliff, WH=7005075155en_HK
dc.identifier.scopusauthoridRommens, JM=7006884140en_HK
dc.identifier.scopusauthoridMarvin, SA=7003285865en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridCollins, FS=7403031285en_HK
dc.identifier.scopusauthoridFrizzell, RA=7005033460en_HK
dc.identifier.scopusauthoridWilson, JM=35452154800en_HK

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