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Article: Isolation of clones on chromosome 7 that contain recognition sites for rare-cutting enzymes by oligonucleotide hybridization

TitleIsolation of clones on chromosome 7 that contain recognition sites for rare-cutting enzymes by oligonucleotide hybridization
Authors
Issue Date1990
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygeno
Citation
Genomics, 1990, v. 7 n. 2, p. 173-181 How to Cite?
AbstractFive G/C-containing oligonucleotides that include the recognition sequences of rare-cutting restriction enzymes have been used to isolate almost 100 different genomic segments from chromosome 7 that contain recognition sites for those enzymes. Hybridization and washing at 27°C allow the use of 8-bp radiolabeled oligonucleotides to detect specific G/C-containing sequences in less than 1 ng of cloned DNA. This method was used to isolate 9 positive clones from 138 previously isolated single-copy probes from a flow-sorted chromosome 7 library. The specificity of the method was confirmed by showing that clones that gave positive hybridization signals also contained the corresponding restriction site. The oligonucleotides were also used to analyze approximately 12,000 kb of genomic sequence from a newly constructed chromosome 7 cosmid library that yielded 88 positive cosmids from 350 analyzed. The average distances between binding sites ranged from 200 to 690 kb and was independent of the number of CpG residues present in the oligonucleotide. Confirmation that clones containing restriction sites for these rare-cutting enzymes are located near genes was obtained by hybridization to RNA and cross-species DNA blots.
Persistent Identifierhttp://hdl.handle.net/10722/44237
ISSN
2015 Impact Factor: 2.386
2015 SCImago Journal Rankings: 1.281
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMelmer, Gen_HK
dc.contributor.authorSood, Ren_HK
dc.contributor.authorRommens, Jen_HK
dc.contributor.authorRego, Den_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorBuchwald, Men_HK
dc.date.accessioned2007-09-12T03:49:37Z-
dc.date.available2007-09-12T03:49:37Z-
dc.date.issued1990en_HK
dc.identifier.citationGenomics, 1990, v. 7 n. 2, p. 173-181en_HK
dc.identifier.issn0888-7543en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44237-
dc.description.abstractFive G/C-containing oligonucleotides that include the recognition sequences of rare-cutting restriction enzymes have been used to isolate almost 100 different genomic segments from chromosome 7 that contain recognition sites for those enzymes. Hybridization and washing at 27°C allow the use of 8-bp radiolabeled oligonucleotides to detect specific G/C-containing sequences in less than 1 ng of cloned DNA. This method was used to isolate 9 positive clones from 138 previously isolated single-copy probes from a flow-sorted chromosome 7 library. The specificity of the method was confirmed by showing that clones that gave positive hybridization signals also contained the corresponding restriction site. The oligonucleotides were also used to analyze approximately 12,000 kb of genomic sequence from a newly constructed chromosome 7 cosmid library that yielded 88 positive cosmids from 350 analyzed. The average distances between binding sites ranged from 200 to 690 kb and was independent of the number of CpG residues present in the oligonucleotide. Confirmation that clones containing restriction sites for these rare-cutting enzymes are located near genes was obtained by hybridization to RNA and cross-species DNA blots.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygenoen_HK
dc.relation.ispartofGenomicsen_HK
dc.subject.meshChromosomes, human, pair 7en_HK
dc.subject.meshCloning, molecularen_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshDna restriction enzymes - metabolismen_HK
dc.subject.meshFlow cytometryen_HK
dc.titleIsolation of clones on chromosome 7 that contain recognition sites for rare-cutting enzymes by oligonucleotide hybridizationen_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1016/0888-7543(90)90538-6en_HK
dc.identifier.pmid2161402-
dc.identifier.scopuseid_2-s2.0-0025285348en_HK
dc.identifier.volume7en_HK
dc.identifier.issue2en_HK
dc.identifier.spage173en_HK
dc.identifier.epage181en_HK
dc.identifier.isiWOS:A1990DE81500005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMelmer, G=7003309613en_HK
dc.identifier.scopusauthoridSood, R=7102088007en_HK
dc.identifier.scopusauthoridRommens, J=7006884140en_HK
dc.identifier.scopusauthoridRego, D=6603959475en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridBuchwald, M=7006759922en_HK

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