Article: Identification of the cystic fibrosis gene: Cloning and characterization of complementary DNA

File Download Links for fulltext
(May Require Subscription)
Supplementary
  • Basic View
  • Metadata View
  • XML View
TitleIdentification of the cystic fibrosis gene: Cloning and characterization of complementary DNA
AuthorsRiordan, JR1
Rommens, JM1
Kerem, BS1
Alon, N1
Rozmahel, R1
Grzelczak, Z1
Zielinski, J1
Lok, S1
Plavsic, N1
Chou, JL1
Drumm, ML1
Iannuzzi, MC1
Collins, FS1
Tsui, LC1
Issue Date1989
PublisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
CitationScience, 1989, v. 245 n. 4922, p. 1066-1073 [How to Cite?]
DOI: http://dx.doi.org/10.1126/science.2475911
AbstractOverlapping complementary DNA clones were isolated from epithelial cell libraries with a genomic DNA segment containing a portion of the putative cystic fibrosis (CF) locus, which is on chromosome 7. Transcripts, approximately 6500 nucleotides in size, were detected in the tissues affected in patients with CF. The predicted protein consists of two similar motifs, each with (i) a domain having properties consistent with membrane association and (ii) a domain believed to be involved in ATP (adenosine triphosphate) binding. A deletion of three base pairs that results in the omission of a phenylalanine residue at the center of the first predicted nucleotide-binding domain was detected in CF patients.
ISSN0036-8075
2011 Impact Factor: 31.201
2011 SCImago Journal Rankings: 5.425
DOIhttp://dx.doi.org/10.1126/science.2475911
ISI Accession Number IDWOS:A1989AN70200026
DC Field
Value
dc.contributor.authorRiordan, JR
dc.contributor.authorRommens, JM
dc.contributor.authorKerem, BS
dc.contributor.authorAlon, N
dc.contributor.authorRozmahel, R
dc.contributor.authorGrzelczak, Z
dc.contributor.authorZielinski, J
dc.contributor.authorLok, S
dc.contributor.authorPlavsic, N
dc.contributor.authorChou, JL
dc.contributor.authorDrumm, ML
dc.contributor.authorIannuzzi, MC
dc.contributor.authorCollins, FS
dc.contributor.authorTsui, LC
dc.date.accessioned2007-09-12T03:49:35Z
dc.date.available2007-09-12T03:49:35Z
dc.date.issued1989
dc.description.abstractOverlapping complementary DNA clones were isolated from epithelial cell libraries with a genomic DNA segment containing a portion of the putative cystic fibrosis (CF) locus, which is on chromosome 7. Transcripts, approximately 6500 nucleotides in size, were detected in the tissues affected in patients with CF. The predicted protein consists of two similar motifs, each with (i) a domain having properties consistent with membrane association and (ii) a domain believed to be involved in ATP (adenosine triphosphate) binding. A deletion of three base pairs that results in the omission of a phenylalanine residue at the center of the first predicted nucleotide-binding domain was detected in CF patients.
dc.description.natureabstract
dc.identifier.citationScience, 1989, v. 245 n. 4922, p. 1066-1073 [How to Cite?]
DOI: http://dx.doi.org/10.1126/science.2475911
dc.identifier.citeulike3717390
dc.identifier.doihttp://dx.doi.org/10.1126/science.2475911
dc.identifier.epage1073
dc.identifier.isiWOS:A1989AN70200026
dc.identifier.issn0036-8075
2011 Impact Factor: 31.201
2011 SCImago Journal Rankings: 5.425
dc.identifier.issue4922
dc.identifier.openurl
dc.identifier.pmid2475911
dc.identifier.scopuseid_2-s2.0-0024424270
dc.identifier.spage1066
dc.identifier.urihttp://hdl.handle.net/10722/44235
dc.identifier.volume245
dc.languageeng
dc.publisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
dc.publisher.placeUnited States
dc.relation.ispartofScience
dc.rightsScience. Copyright © American Association for the Advancement of Science.
dc.subject.meshAmino acid sequence
dc.subject.meshBiological transport
dc.subject.meshCloning, molecular - methods
dc.subject.meshCystic fibrosis - genetics - metabolism - pathology
dc.subject.meshCystic fibrosis transmembrane conductance regulator
dc.titleIdentification of the cystic fibrosis gene: Cloning and characterization of complementary DNA
dc.typeArticle
Author Affiliations
  1. Hospital for Sick Children, Toronto