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Article: Identification of the cystic fibrosis gene: Cloning and characterization of complementary DNA
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TitleIdentification of the cystic fibrosis gene: Cloning and characterization of complementary DNA
 
AuthorsRiordan, JR1
Rommens, JM1
Kerem, BS1
Alon, N1
Rozmahel, R1
Grzelczak, Z1
Zielinski, J1
Lok, S1
Plavsic, N1
Chou, JL1
Drumm, ML1
Iannuzzi, MC1
Collins, FS1
Tsui, LC1
 
Issue Date1989
 
PublisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
 
CitationScience, 1989, v. 245 n. 4922, p. 1066-1073 [How to Cite?]
DOI: http://dx.doi.org/10.1126/science.2475911
 
AbstractOverlapping complementary DNA clones were isolated from epithelial cell libraries with a genomic DNA segment containing a portion of the putative cystic fibrosis (CF) locus, which is on chromosome 7. Transcripts, approximately 6500 nucleotides in size, were detected in the tissues affected in patients with CF. The predicted protein consists of two similar motifs, each with (i) a domain having properties consistent with membrane association and (ii) a domain believed to be involved in ATP (adenosine triphosphate) binding. A deletion of three base pairs that results in the omission of a phenylalanine residue at the center of the first predicted nucleotide-binding domain was detected in CF patients.
 
ISSN0036-8075
2013 Impact Factor: 31.477
2013 SCImago Journal Rankings: 12.465
 
DOIhttp://dx.doi.org/10.1126/science.2475911
 
ISI Accession Number IDWOS:A1989AN70200026
 
DC FieldValue
dc.contributor.authorRiordan, JR
 
dc.contributor.authorRommens, JM
 
dc.contributor.authorKerem, BS
 
dc.contributor.authorAlon, N
 
dc.contributor.authorRozmahel, R
 
dc.contributor.authorGrzelczak, Z
 
dc.contributor.authorZielinski, J
 
dc.contributor.authorLok, S
 
dc.contributor.authorPlavsic, N
 
dc.contributor.authorChou, JL
 
dc.contributor.authorDrumm, ML
 
dc.contributor.authorIannuzzi, MC
 
dc.contributor.authorCollins, FS
 
dc.contributor.authorTsui, LC
 
dc.date.accessioned2007-09-12T03:49:35Z
 
dc.date.available2007-09-12T03:49:35Z
 
dc.date.issued1989
 
dc.description.abstractOverlapping complementary DNA clones were isolated from epithelial cell libraries with a genomic DNA segment containing a portion of the putative cystic fibrosis (CF) locus, which is on chromosome 7. Transcripts, approximately 6500 nucleotides in size, were detected in the tissues affected in patients with CF. The predicted protein consists of two similar motifs, each with (i) a domain having properties consistent with membrane association and (ii) a domain believed to be involved in ATP (adenosine triphosphate) binding. A deletion of three base pairs that results in the omission of a phenylalanine residue at the center of the first predicted nucleotide-binding domain was detected in CF patients.
 
dc.description.natureabstract
 
dc.identifier.citationScience, 1989, v. 245 n. 4922, p. 1066-1073 [How to Cite?]
DOI: http://dx.doi.org/10.1126/science.2475911
 
dc.identifier.citeulike3717390
 
dc.identifier.doihttp://dx.doi.org/10.1126/science.2475911
 
dc.identifier.epage1073
 
dc.identifier.isiWOS:A1989AN70200026
 
dc.identifier.issn0036-8075
2013 Impact Factor: 31.477
2013 SCImago Journal Rankings: 12.465
 
dc.identifier.issue4922
 
dc.identifier.openurl
 
dc.identifier.pmid2475911
 
dc.identifier.scopuseid_2-s2.0-0024424270
 
dc.identifier.spage1066
 
dc.identifier.urihttp://hdl.handle.net/10722/44235
 
dc.identifier.volume245
 
dc.languageeng
 
dc.publisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
 
dc.publisher.placeUnited States
 
dc.relation.ispartofScience
 
dc.rightsScience. Copyright © American Association for the Advancement of Science.
 
dc.subject.meshAmino acid sequence
 
dc.subject.meshBiological transport
 
dc.subject.meshCloning, molecular - methods
 
dc.subject.meshCystic fibrosis - genetics - metabolism - pathology
 
dc.subject.meshCystic fibrosis transmembrane conductance regulator
 
dc.titleIdentification of the cystic fibrosis gene: Cloning and characterization of complementary DNA
 
dc.typeArticle
 
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<contributor.author>Alon, N</contributor.author>
<contributor.author>Rozmahel, R</contributor.author>
<contributor.author>Grzelczak, Z</contributor.author>
<contributor.author>Zielinski, J</contributor.author>
<contributor.author>Lok, S</contributor.author>
<contributor.author>Plavsic, N</contributor.author>
<contributor.author>Chou, JL</contributor.author>
<contributor.author>Drumm, ML</contributor.author>
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Author Affiliations
  1. Hospital for Sick Children University of Toronto