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Article: Identification of the cystic fibrosis gene: Chromosome walking and jumping
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TitleIdentification of the cystic fibrosis gene: Chromosome walking and jumping
 
AuthorsRommens, JM1
Iannuzzi, MC1
Kerem, BS1
Drumm, ML1
Melmer, G1
Dean, M1
Rozmahel, R1
Cole, JL1
Kennedy, D1
Hidaka, N1
Zsiga, M1
Buchwald, M1
Riordan, JR1
Tsui, LC1
Collins, FS1
 
Issue Date1989
 
PublisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
 
CitationScience, 1989, v. 245 n. 4922, p. 1059-1065 [How to Cite?]
DOI: http://dx.doi.org/10.1126/science.2772657
 
AbstractAn understanding of the basic defect in the inherited disorder cystic fibrosis requires cloning of the cystic fibrosis gene and definition of its protein product. In the absence of direct functional information, chromosomal map position is a guide for locating the gene. Chromosome walking and jumping and complementary DNA hybridization were used to isolate DNA sequences, encompassing more than 500,000 base pairs, from the cystic fibrosis region on the long arm of human chromosome 7. Several transcribed sequences and conserved segments were identified in this cloned region. One of these corresponds to the cystic fibrosis gene and spans approximately 250,000 base pairs of genomic DNA.
 
ISSN0036-8075
2012 Impact Factor: 31.027
2012 SCImago Journal Rankings: 10.618
 
DOIhttp://dx.doi.org/10.1126/science.2772657
 
ISI Accession Number IDWOS:A1989AN70200025
 
DC FieldValue
dc.contributor.authorRommens, JM
 
dc.contributor.authorIannuzzi, MC
 
dc.contributor.authorKerem, BS
 
dc.contributor.authorDrumm, ML
 
dc.contributor.authorMelmer, G
 
dc.contributor.authorDean, M
 
dc.contributor.authorRozmahel, R
 
dc.contributor.authorCole, JL
 
dc.contributor.authorKennedy, D
 
dc.contributor.authorHidaka, N
 
dc.contributor.authorZsiga, M
 
dc.contributor.authorBuchwald, M
 
dc.contributor.authorRiordan, JR
 
dc.contributor.authorTsui, LC
 
dc.contributor.authorCollins, FS
 
dc.date.accessioned2007-09-12T03:49:33Z
 
dc.date.available2007-09-12T03:49:33Z
 
dc.date.issued1989
 
dc.description.abstractAn understanding of the basic defect in the inherited disorder cystic fibrosis requires cloning of the cystic fibrosis gene and definition of its protein product. In the absence of direct functional information, chromosomal map position is a guide for locating the gene. Chromosome walking and jumping and complementary DNA hybridization were used to isolate DNA sequences, encompassing more than 500,000 base pairs, from the cystic fibrosis region on the long arm of human chromosome 7. Several transcribed sequences and conserved segments were identified in this cloned region. One of these corresponds to the cystic fibrosis gene and spans approximately 250,000 base pairs of genomic DNA.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationScience, 1989, v. 245 n. 4922, p. 1059-1065 [How to Cite?]
DOI: http://dx.doi.org/10.1126/science.2772657
 
dc.identifier.doihttp://dx.doi.org/10.1126/science.2772657
 
dc.identifier.epage1065
 
dc.identifier.isiWOS:A1989AN70200025
 
dc.identifier.issn0036-8075
2012 Impact Factor: 31.027
2012 SCImago Journal Rankings: 10.618
 
dc.identifier.issue4922
 
dc.identifier.openurl
 
dc.identifier.pmid2772657
 
dc.identifier.scopuseid_2-s2.0-0024453308
 
dc.identifier.spage1059
 
dc.identifier.urihttp://hdl.handle.net/10722/44234
 
dc.identifier.volume245
 
dc.languageeng
 
dc.publisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
 
dc.publisher.placeUnited States
 
dc.relation.ispartofScience
 
dc.rightsScience. Copyright © American Association for the Advancement of Science.
 
dc.subject.meshChromosome mapping
 
dc.subject.meshChromosomes, human, pair 7
 
dc.subject.meshCloning, molecular - methods
 
dc.subject.meshCricetinae
 
dc.subject.meshCystic fibrosis - genetics
 
dc.titleIdentification of the cystic fibrosis gene: Chromosome walking and jumping
 
dc.typeArticle
 
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<contributor.author>Melmer, G</contributor.author>
<contributor.author>Dean, M</contributor.author>
<contributor.author>Rozmahel, R</contributor.author>
<contributor.author>Cole, JL</contributor.author>
<contributor.author>Kennedy, D</contributor.author>
<contributor.author>Hidaka, N</contributor.author>
<contributor.author>Zsiga, M</contributor.author>
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Author Affiliations
  1. Hospital for Sick Children University of Toronto