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Article: Identification of the cystic fibrosis gene: Chromosome walking and jumping

TitleIdentification of the cystic fibrosis gene: Chromosome walking and jumping
Authors
Issue Date1989
PublisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
Citation
Science, 1989, v. 245 n. 4922, p. 1059-1065 How to Cite?
Abstract
An understanding of the basic defect in the inherited disorder cystic fibrosis requires cloning of the cystic fibrosis gene and definition of its protein product. In the absence of direct functional information, chromosomal map position is a guide for locating the gene. Chromosome walking and jumping and complementary DNA hybridization were used to isolate DNA sequences, encompassing more than 500,000 base pairs, from the cystic fibrosis region on the long arm of human chromosome 7. Several transcribed sequences and conserved segments were identified in this cloned region. One of these corresponds to the cystic fibrosis gene and spans approximately 250,000 base pairs of genomic DNA.
Persistent Identifierhttp://hdl.handle.net/10722/44234
ISSN
2013 Impact Factor: 31.477
2013 SCImago Journal Rankings: 12.465
ISI Accession Number ID

 

Author Affiliations
  1. Hospital for Sick Children University of Toronto
DC FieldValueLanguage
dc.contributor.authorRommens, JMen_HK
dc.contributor.authorIannuzzi, MCen_HK
dc.contributor.authorKerem, BSen_HK
dc.contributor.authorDrumm, MLen_HK
dc.contributor.authorMelmer, Gen_HK
dc.contributor.authorDean, Men_HK
dc.contributor.authorRozmahel, Ren_HK
dc.contributor.authorCole, JLen_HK
dc.contributor.authorKennedy, Den_HK
dc.contributor.authorHidaka, Nen_HK
dc.contributor.authorZsiga, Men_HK
dc.contributor.authorBuchwald, Men_HK
dc.contributor.authorRiordan, JRen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorCollins, FSen_HK
dc.date.accessioned2007-09-12T03:49:33Z-
dc.date.available2007-09-12T03:49:33Z-
dc.date.issued1989en_HK
dc.identifier.citationScience, 1989, v. 245 n. 4922, p. 1059-1065en_HK
dc.identifier.issn0036-8075en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44234-
dc.description.abstractAn understanding of the basic defect in the inherited disorder cystic fibrosis requires cloning of the cystic fibrosis gene and definition of its protein product. In the absence of direct functional information, chromosomal map position is a guide for locating the gene. Chromosome walking and jumping and complementary DNA hybridization were used to isolate DNA sequences, encompassing more than 500,000 base pairs, from the cystic fibrosis region on the long arm of human chromosome 7. Several transcribed sequences and conserved segments were identified in this cloned region. One of these corresponds to the cystic fibrosis gene and spans approximately 250,000 base pairs of genomic DNA.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.orgen_HK
dc.relation.ispartofScienceen_HK
dc.rightsScience. Copyright © American Association for the Advancement of Science.en_HK
dc.subject.meshChromosome mappingen_HK
dc.subject.meshChromosomes, human, pair 7en_HK
dc.subject.meshCloning, molecular - methodsen_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshCystic fibrosis - geneticsen_HK
dc.titleIdentification of the cystic fibrosis gene: Chromosome walking and jumpingen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0036-8075&volume=245&issue=4922&spage=1059&epage=1065&date=1989&atitle=Identification+of+the+cystic+fibrosis+gene:+chromosome+walking+and+jumpingen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_OA_fulltexten_HK
dc.identifier.doi10.1126/science.2772657-
dc.identifier.pmid2772657en_HK
dc.identifier.scopuseid_2-s2.0-0024453308en_HK
dc.identifier.volume245en_HK
dc.identifier.issue4922en_HK
dc.identifier.spage1059en_HK
dc.identifier.epage1065en_HK
dc.identifier.isiWOS:A1989AN70200025-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridRommens, JM=7006884140en_HK
dc.identifier.scopusauthoridIannuzzi, MC=7006338485en_HK
dc.identifier.scopusauthoridKerem, BS=35376353800en_HK
dc.identifier.scopusauthoridDrumm, ML=7006422406en_HK
dc.identifier.scopusauthoridMelmer, G=7003309613en_HK
dc.identifier.scopusauthoridDean, M=35374417600en_HK
dc.identifier.scopusauthoridRozmahel, R=6701510561en_HK
dc.identifier.scopusauthoridCole, JL=7403376226en_HK
dc.identifier.scopusauthoridKennedy, D=7403112289en_HK
dc.identifier.scopusauthoridHidaka, N=7006119259en_HK
dc.identifier.scopusauthoridZsiga, M=7801563333en_HK
dc.identifier.scopusauthoridBuchwald, M=7006759922en_HK
dc.identifier.scopusauthoridRiordan, JR=7202229758en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridCollins, FS=7403031285en_HK

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