Article: Identification of the cystic fibrosis gene: Chromosome walking and jumping

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TitleIdentification of the cystic fibrosis gene: Chromosome walking and jumping
AuthorsRommens, JM1
Iannuzzi, MC1
Kerem, BS1
Drumm, ML1
Melmer, G1
Dean, M1
Rozmahel, R1
Cole, JL1
Kennedy, D1
Hidaka, N1
Zsiga, M1
Buchwald, M1
Riordan, JR1
Tsui, LC1
Collins, FS1
Issue Date1989
PublisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
CitationScience, 1989, v. 245 n. 4922, p. 1059-1065 [How to Cite?]
DOI: http://dx.doi.org/10.1126/science.2772657
AbstractAn understanding of the basic defect in the inherited disorder cystic fibrosis requires cloning of the cystic fibrosis gene and definition of its protein product. In the absence of direct functional information, chromosomal map position is a guide for locating the gene. Chromosome walking and jumping and complementary DNA hybridization were used to isolate DNA sequences, encompassing more than 500,000 base pairs, from the cystic fibrosis region on the long arm of human chromosome 7. Several transcribed sequences and conserved segments were identified in this cloned region. One of these corresponds to the cystic fibrosis gene and spans approximately 250,000 base pairs of genomic DNA.
ISSN0036-8075
2011 Impact Factor: 31.201
2011 SCImago Journal Rankings: 5.425
DOIhttp://dx.doi.org/10.1126/science.2772657
ISI Accession Number IDWOS:A1989AN70200025
DC Field
Value
dc.contributor.authorRommens, JM
dc.contributor.authorIannuzzi, MC
dc.contributor.authorKerem, BS
dc.contributor.authorDrumm, ML
dc.contributor.authorMelmer, G
dc.contributor.authorDean, M
dc.contributor.authorRozmahel, R
dc.contributor.authorCole, JL
dc.contributor.authorKennedy, D
dc.contributor.authorHidaka, N
dc.contributor.authorZsiga, M
dc.contributor.authorBuchwald, M
dc.contributor.authorRiordan, JR
dc.contributor.authorTsui, LC
dc.contributor.authorCollins, FS
dc.date.accessioned2007-09-12T03:49:33Z
dc.date.available2007-09-12T03:49:33Z
dc.date.issued1989
dc.description.abstractAn understanding of the basic defect in the inherited disorder cystic fibrosis requires cloning of the cystic fibrosis gene and definition of its protein product. In the absence of direct functional information, chromosomal map position is a guide for locating the gene. Chromosome walking and jumping and complementary DNA hybridization were used to isolate DNA sequences, encompassing more than 500,000 base pairs, from the cystic fibrosis region on the long arm of human chromosome 7. Several transcribed sequences and conserved segments were identified in this cloned region. One of these corresponds to the cystic fibrosis gene and spans approximately 250,000 base pairs of genomic DNA.
dc.description.naturelink_to_OA_fulltext
dc.identifier.citationScience, 1989, v. 245 n. 4922, p. 1059-1065 [How to Cite?]
DOI: http://dx.doi.org/10.1126/science.2772657
dc.identifier.doihttp://dx.doi.org/10.1126/science.2772657
dc.identifier.epage1065
dc.identifier.isiWOS:A1989AN70200025
dc.identifier.issn0036-8075
2011 Impact Factor: 31.201
2011 SCImago Journal Rankings: 5.425
dc.identifier.issue4922
dc.identifier.openurl
dc.identifier.pmid2772657
dc.identifier.scopuseid_2-s2.0-0024453308
dc.identifier.spage1059
dc.identifier.urihttp://hdl.handle.net/10722/44234
dc.identifier.volume245
dc.languageeng
dc.publisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
dc.publisher.placeUnited States
dc.relation.ispartofScience
dc.rightsScience. Copyright © American Association for the Advancement of Science.
dc.subject.meshChromosome mapping
dc.subject.meshChromosomes, human, pair 7
dc.subject.meshCloning, molecular - methods
dc.subject.meshCricetinae
dc.subject.meshCystic fibrosis - genetics
dc.titleIdentification of the cystic fibrosis gene: Chromosome walking and jumping
dc.typeArticle
Author Affiliations
  1. Hospital for Sick Children, Toronto