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Article: Multiple regulatory elements of the murine γ2-crystallin promoter

TitleMultiple regulatory elements of the murine γ2-crystallin promoter
Authors
Issue Date1989
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 1989, v. 17 n. 9, p. 3563-3582 How to Cite?
AbstractCrystallins are the major water-soluble proteins of the vertebrate eye lens. These lens-specific proteins are encoded by several multi-gene families whose expression is differentially regulated during development. Our previous studies showed that the mouse gamma 2-crystallin promoter is active on transfection into lens-explant cultures derived from 14-day-old chick embryos but not on transfection into a variety of non-lens cells. In this study, transient expression data show that a sequence of 226 nucleotides upstream from the transcription start site is sufficient for activity of this promoter in the chicken lens cells. This sequence can be further divided into two domains, A and B, both of which are required for promoter function. Domain A (nucleotide -68 to -18) contains the TATA box and sequence motifs that are conserved in all gamma-crystallin promoters. Domain B (-226 to -120) consists of three regions. One of these regions contains an element with dyad symmetry and a sequence similar to the octamer motif. The second region contains an enhancer core consensus sequence. Two 'enhancer-like' activities have been detected, one in Domain B and a second in a more distal region (-392 to -278) that does not appear to be required for promoter activity in transfection assays.
Persistent Identifierhttp://hdl.handle.net/10722/44231
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLok, Sen_HK
dc.contributor.authorStevens, Wen_HK
dc.contributor.authorBreitman, MLen_HK
dc.contributor.authorTsui, LCen_HK
dc.date.accessioned2007-09-12T03:49:29Z-
dc.date.available2007-09-12T03:49:29Z-
dc.date.issued1989en_HK
dc.identifier.citationNucleic Acids Research, 1989, v. 17 n. 9, p. 3563-3582en_HK
dc.identifier.issn0305-1048en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44231-
dc.description.abstractCrystallins are the major water-soluble proteins of the vertebrate eye lens. These lens-specific proteins are encoded by several multi-gene families whose expression is differentially regulated during development. Our previous studies showed that the mouse gamma 2-crystallin promoter is active on transfection into lens-explant cultures derived from 14-day-old chick embryos but not on transfection into a variety of non-lens cells. In this study, transient expression data show that a sequence of 226 nucleotides upstream from the transcription start site is sufficient for activity of this promoter in the chicken lens cells. This sequence can be further divided into two domains, A and B, both of which are required for promoter function. Domain A (nucleotide -68 to -18) contains the TATA box and sequence motifs that are conserved in all gamma-crystallin promoters. Domain B (-226 to -120) consists of three regions. One of these regions contains an element with dyad symmetry and a sequence similar to the octamer motif. The second region contains an enhancer core consensus sequence. Two 'enhancer-like' activities have been detected, one in Domain B and a second in a more distal region (-392 to -278) that does not appear to be required for promoter activity in transfection assays.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_HK
dc.relation.ispartofNucleic Acids Researchen_HK
dc.subject.meshChick embryoen_HK
dc.subject.meshLens, crystalline - metabolismen_HK
dc.subject.meshCrystallins - geneticsen_HK
dc.subject.meshGenes, regulatoren_HK
dc.subject.meshMultigene familyen_HK
dc.titleMultiple regulatory elements of the murine γ2-crystallin promoteren_HK
dc.typeArticleen_HK
dc.identifier.emailLok, S: silok@genome.hku.hken_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityLok, S=rp00271en_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_OA_fulltexten_HK
dc.identifier.doi10.1093/nar/17.9.3563-
dc.identifier.pmid2726487-
dc.identifier.pmcidPMC317796-
dc.identifier.scopuseid_2-s2.0-0024560880en_HK
dc.identifier.volume17en_HK
dc.identifier.issue9en_HK
dc.identifier.spage3563en_HK
dc.identifier.epage3582en_HK
dc.identifier.isiWOS:A1989U545200019-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLok, S=21035019900en_HK
dc.identifier.scopusauthoridStevens, W=55424060700en_HK
dc.identifier.scopusauthoridBreitman, ML=7005448008en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK

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