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Article: A rabbit lens epithelial cell line supports expression of an exogenous crystallin gene characteristic of lens fiber cell differentiation

TitleA rabbit lens epithelial cell line supports expression of an exogenous crystallin gene characteristic of lens fiber cell differentiation
Authors
Issue Date1989
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexer
Citation
Experimental Eye Research, 1989, v. 48 n. 1, p. 131-137 How to Cite?
AbstractCell lines derived from the lens generally fail to maintain synthesis of crystallins in long-term culture. Here we demonstrate that the N/N1003A line of undifferentiated lens epithelial cells, derived from a newborn rabbit, does not produce detectable levels of α, β or γ-crystallin transcripts, yet is capable of supporting the transient expression of the mouse γ2-crystallin promoter, a promoter which is active only in terminally differentiated cells in vivo. Analysis of a set deletion constructs suggested that sequences required for activity of the mouse promoter in N/N1003A cells are similar, but not identical, to those previously shown to be essential in primary chick embryo lens explants. Therefore, these results suggest that different transcriptional factors may be capable of supporting lens-specific activity of the mouse γ2 promoter. In addition, this cell line, N/N1003A, should be useful for investigations on the elements regulating γ-crystallin gene expression.
Persistent Identifierhttp://hdl.handle.net/10722/44229
ISSN
2015 Impact Factor: 2.998
2015 SCImago Journal Rankings: 1.218
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMeakin, SOen_HK
dc.contributor.authorReddan, JRen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorBreitman, MLen_HK
dc.date.accessioned2007-09-12T03:49:26Z-
dc.date.available2007-09-12T03:49:26Z-
dc.date.issued1989en_HK
dc.identifier.citationExperimental Eye Research, 1989, v. 48 n. 1, p. 131-137en_HK
dc.identifier.issn0014-4835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44229-
dc.description.abstractCell lines derived from the lens generally fail to maintain synthesis of crystallins in long-term culture. Here we demonstrate that the N/N1003A line of undifferentiated lens epithelial cells, derived from a newborn rabbit, does not produce detectable levels of α, β or γ-crystallin transcripts, yet is capable of supporting the transient expression of the mouse γ2-crystallin promoter, a promoter which is active only in terminally differentiated cells in vivo. Analysis of a set deletion constructs suggested that sequences required for activity of the mouse promoter in N/N1003A cells are similar, but not identical, to those previously shown to be essential in primary chick embryo lens explants. Therefore, these results suggest that different transcriptional factors may be capable of supporting lens-specific activity of the mouse γ2 promoter. In addition, this cell line, N/N1003A, should be useful for investigations on the elements regulating γ-crystallin gene expression.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexeren_HK
dc.relation.ispartofExperimental Eye Researchen_HK
dc.subject.meshCell differentiationen_HK
dc.subject.meshChick embryoen_HK
dc.subject.meshCrystallins - biosynthesisen_HK
dc.subject.meshEpithelium - metabolismen_HK
dc.subject.meshLens, crystalline - cytologyen_HK
dc.titleA rabbit lens epithelial cell line supports expression of an exogenous crystallin gene characteristic of lens fiber cell differentiationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-4835&volume=48&issue=1&spage=131&epage=137&date=1989&atitle=A+rabbit+lens+epithelial+cell+line+supports+expression+of+an+exogenous+crystallin+gene+characteristic+of+lens+fiber+cell+differentiationen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1016/0014-4835(89)90026-2en_HK
dc.identifier.pmid2920780-
dc.identifier.scopuseid_2-s2.0-0024490930en_HK
dc.identifier.volume48en_HK
dc.identifier.issue1en_HK
dc.identifier.spage131en_HK
dc.identifier.epage137en_HK
dc.identifier.isiWOS:A1989R754500014-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridMeakin, SO=7006735265en_HK
dc.identifier.scopusauthoridReddan, JR=7006372927en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridBreitman, ML=7005448008en_HK

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