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Article: Genetic ablation: Targeted expression of a toxin gene causes microphthalmia in transgenic mice

TitleGenetic ablation: Targeted expression of a toxin gene causes microphthalmia in transgenic mice
Authors
Issue Date1987
PublisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
Citation
Science, 1987, v. 238 n. 4833, p. 1563-1565 How to Cite?
AbstractLineage-specific regulatory elements can be used to direct expression of a variety of genes to specific tissues in transgenic mice. If the hybrid constructs contain a gene encoding a cytotoxic gene product, then genetic ablation of a specific cell lineage can be achieved. We have generated six transgenic mice by introducing into fertilized eggs the mouse γ2-crystallin promoter fused to the coding region of the diphtheria toxin A-chain gene. Three of these mice and all the transgenic offspring analyzed were microphthalmic. The lenses of these mice displayed considerable heterogeneity: some were almost normal morphologically but reduced in size, whereas others were grossly aberrant and deficient in nuclear fiber cells. These studies indicate that programmed ablation of specific cell types can be stably transmitted through the germ line.
Persistent Identifierhttp://hdl.handle.net/10722/44226
ISSN
2015 Impact Factor: 34.661
2015 SCImago Journal Rankings: 13.217
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBreitman, MLen_HK
dc.contributor.authorClapoff, Sen_HK
dc.contributor.authorRossant, Jen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorGlode, LMen_HK
dc.contributor.authorMaxwell, IHen_HK
dc.contributor.authorBernstein, Aen_HK
dc.date.accessioned2007-09-12T03:49:23Z-
dc.date.available2007-09-12T03:49:23Z-
dc.date.issued1987en_HK
dc.identifier.citationScience, 1987, v. 238 n. 4833, p. 1563-1565en_HK
dc.identifier.issn0036-8075en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44226-
dc.description.abstractLineage-specific regulatory elements can be used to direct expression of a variety of genes to specific tissues in transgenic mice. If the hybrid constructs contain a gene encoding a cytotoxic gene product, then genetic ablation of a specific cell lineage can be achieved. We have generated six transgenic mice by introducing into fertilized eggs the mouse γ2-crystallin promoter fused to the coding region of the diphtheria toxin A-chain gene. Three of these mice and all the transgenic offspring analyzed were microphthalmic. The lenses of these mice displayed considerable heterogeneity: some were almost normal morphologically but reduced in size, whereas others were grossly aberrant and deficient in nuclear fiber cells. These studies indicate that programmed ablation of specific cell types can be stably transmitted through the germ line.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.orgen_HK
dc.relation.ispartofScienceen_HK
dc.rightsScience. Copyright © American Association for the Advancement of Science.en_HK
dc.subject.meshCrystallins - geneticsen_HK
dc.subject.meshDiphtheria toxin - geneticsen_HK
dc.subject.meshEye - pathologyen_HK
dc.subject.meshMicrophthalmos - genetics - pathologyen_HK
dc.subject.meshMice, transgenicen_HK
dc.titleGenetic ablation: Targeted expression of a toxin gene causes microphthalmia in transgenic miceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0036-8075&volume=238&issue=4833&spage=1563&epage=1565&date=1987&atitle=Genetic+ablation:+Targeted+expression+of+a+toxin+gene+causes+microphthalmia+in+transgenic+miceen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.pmid3685993-
dc.identifier.scopuseid_2-s2.0-0023631457en_HK
dc.identifier.volume238en_HK
dc.identifier.issue4833en_HK
dc.identifier.spage1563en_HK
dc.identifier.epage1565en_HK
dc.identifier.isiWOS:A1987L178900043-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridBreitman, ML=7005448008en_HK
dc.identifier.scopusauthoridClapoff, S=6508066159en_HK
dc.identifier.scopusauthoridRossant, J=35392915000en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridGlode, LM=7005204065en_HK
dc.identifier.scopusauthoridMaxwell, IH=7005879119en_HK
dc.identifier.scopusauthoridBernstein, A=35463110000en_HK

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