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Article: Relationship between proteins encoded by three human gamma-crystallin genes and distinct polypeptides in the eye lens.

TitleRelationship between proteins encoded by three human gamma-crystallin genes and distinct polypeptides in the eye lens.
Authors
Issue Date1987
PublisherAmerican Society for Microbiology.
Citation
Molecular And Cellular Biology, 1987, v. 7 n. 9, p. 3320-3323 How to Cite?
AbstractAlthough individual gamma-crystallins from the human eye lens have not been successfully purified and sequenced, most of the genes coding for these lens-specific structural proteins have been cloned and characterized. To investigate the relationship between these genes and the gamma-crystallins of the human lens, we made use of mouse cell lines which contain stably integrated copies of the coding sequences for three of the human gamma-crystallin genes coupled to the human metallothionein IIA promoter. The proteins produced by these hybrid genes in cell culture were detected immunologically and compared by physical characteristics with the gamma-crystallins from the human lens. The protein encoded by the G3 gene showed properties identical to those of the 21,000-molecular-weight gamma-crystallin from 11-month-old lens. The protein isolated from the cells expressing the G4 gene was similar to a 19,000-molecular-weight lens gamma-crystallin, while gene G5 encodes a highly basic gamma-crystallin which may be synthesized in only limited amounts in the human lens. These correlations provide a basis for future investigations on the relationship between putative mutations in human gamma-crystallin genes and altered proteins in hereditary lens cataracts.
Persistent Identifierhttp://hdl.handle.net/10722/44224
ISSN
2015 Impact Factor: 4.427
2015 SCImago Journal Rankings: 3.806
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DC FieldValueLanguage
dc.contributor.authorRussell, Pen_HK
dc.contributor.authorMeakin, SOen_HK
dc.contributor.authorHohman, TCen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorBreitman, MLen_HK
dc.date.accessioned2007-09-12T03:49:21Z-
dc.date.available2007-09-12T03:49:21Z-
dc.date.issued1987en_HK
dc.identifierhttp://mcb.asm.org/cgi/reprint/7/9/3320.pdfen_HK
dc.identifier.citationMolecular And Cellular Biology, 1987, v. 7 n. 9, p. 3320-3323en_HK
dc.identifier.issn0270-7306en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44224-
dc.description.abstractAlthough individual gamma-crystallins from the human eye lens have not been successfully purified and sequenced, most of the genes coding for these lens-specific structural proteins have been cloned and characterized. To investigate the relationship between these genes and the gamma-crystallins of the human lens, we made use of mouse cell lines which contain stably integrated copies of the coding sequences for three of the human gamma-crystallin genes coupled to the human metallothionein IIA promoter. The proteins produced by these hybrid genes in cell culture were detected immunologically and compared by physical characteristics with the gamma-crystallins from the human lens. The protein encoded by the G3 gene showed properties identical to those of the 21,000-molecular-weight gamma-crystallin from 11-month-old lens. The protein isolated from the cells expressing the G4 gene was similar to a 19,000-molecular-weight lens gamma-crystallin, while gene G5 encodes a highly basic gamma-crystallin which may be synthesized in only limited amounts in the human lens. These correlations provide a basis for future investigations on the relationship between putative mutations in human gamma-crystallin genes and altered proteins in hereditary lens cataracts.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofMolecular and Cellular Biologyen_HK
dc.rightsCopyright © American Society for Microbiology, Molecular and cellular biology, 1987, v. 7 n. 9, p. 3320-3323en_HK
dc.subject.meshCrystallins - geneticsen_HK
dc.subject.meshImmunosorbent techniquesen_HK
dc.subject.meshIsoelectric pointen_HK
dc.subject.meshLens, crystalline - physiologyen_HK
dc.subject.meshMolecular weighten_HK
dc.titleRelationship between proteins encoded by three human gamma-crystallin genes and distinct polypeptides in the eye lens.en_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_OA_fulltexten_HK
dc.identifier.pmid3313014-
dc.identifier.pmcidPMC367970-
dc.identifier.scopuseid_2-s2.0-0023408704en_HK
dc.identifier.volume7en_HK
dc.identifier.issue9en_HK
dc.identifier.spage3320en_HK
dc.identifier.epage3323en_HK
dc.identifier.isiWOS:A1987J716400038-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridRussell, P=7401844544en_HK
dc.identifier.scopusauthoridMeakin, SO=7006735265en_HK
dc.identifier.scopusauthoridHohman, TC=35583725600en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridBreitman, ML=7005448008en_HK

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