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Article: Gamma-crystallins of the human eye lens: expression analysis of five members of the gene family.

TitleGamma-crystallins of the human eye lens: expression analysis of five members of the gene family.
Authors
Issue Date1987
PublisherAmerican Society for Microbiology.
Citation
Molecular And Cellular Biology, 1987, v. 7 n. 8, p. 2671-2679 How to Cite?
AbstractWhile only two gamma-crystallins have been identified in the human eye lens, molecular studies indicate that the human gamma-crystallins are encoded in a multigene family comprising at least seven closely related members. Sequence analysis of five of these genes has suggested that three (gamma 1-2, G3, and G4) are potentially active, while two (G1 psi and G2 psi) correspond to closely related pseudogenes. Here we report on the detailed structure of a sixth gamma-crystallin gene, G5, and our results obtained with transient expression assays to characterize both the promoter activity and translation products of five members of the gene family. We show that 5'-flanking sequences of G1 psi and G2 psi lacked detectable promoter activity, while the corresponding sequences of G3, G4, and G5 were able to direct high levels of expression of the bacterial chloramphenicol acetyltransferase gene in primary lens epithelia, but not in cultures of nonlens origin. Detailed sequence comparisons indicated that active genes contained several conserved sequence tracts 5' of the TATA box which may constitute functional elements of a lens-specific gamma-crystallin promoter. Expression of the gamma-crystallin coding sequences from the human metallothionein IIA promoter in nonlens cells facilitated characterization of the polypeptides encoded by individual gamma-genes and, in future studies, should permit comparison of these proteins with distinct gamma-crystallins in the human lens.
Persistent Identifierhttp://hdl.handle.net/10722/44223
ISSN
2015 Impact Factor: 4.427
2015 SCImago Journal Rankings: 3.806
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DC FieldValueLanguage
dc.contributor.authorMeakin, SOen_HK
dc.contributor.authorDu, RPen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorBreitman, MLen_HK
dc.date.accessioned2007-09-12T03:49:20Z-
dc.date.available2007-09-12T03:49:20Z-
dc.date.issued1987en_HK
dc.identifierhttp://mcb.asm.org/cgi/reprint/7/8/2671.pdfen_HK
dc.identifier.citationMolecular And Cellular Biology, 1987, v. 7 n. 8, p. 2671-2679en_HK
dc.identifier.issn0270-7306en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44223-
dc.description.abstractWhile only two gamma-crystallins have been identified in the human eye lens, molecular studies indicate that the human gamma-crystallins are encoded in a multigene family comprising at least seven closely related members. Sequence analysis of five of these genes has suggested that three (gamma 1-2, G3, and G4) are potentially active, while two (G1 psi and G2 psi) correspond to closely related pseudogenes. Here we report on the detailed structure of a sixth gamma-crystallin gene, G5, and our results obtained with transient expression assays to characterize both the promoter activity and translation products of five members of the gene family. We show that 5'-flanking sequences of G1 psi and G2 psi lacked detectable promoter activity, while the corresponding sequences of G3, G4, and G5 were able to direct high levels of expression of the bacterial chloramphenicol acetyltransferase gene in primary lens epithelia, but not in cultures of nonlens origin. Detailed sequence comparisons indicated that active genes contained several conserved sequence tracts 5' of the TATA box which may constitute functional elements of a lens-specific gamma-crystallin promoter. Expression of the gamma-crystallin coding sequences from the human metallothionein IIA promoter in nonlens cells facilitated characterization of the polypeptides encoded by individual gamma-genes and, in future studies, should permit comparison of these proteins with distinct gamma-crystallins in the human lens.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofMolecular and Cellular Biologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Molecular and cellular biology, 1987, v. 7 n. 8, p. 2671-2679en_HK
dc.subject.meshAmino acid sequenceen_HK
dc.subject.meshCloning, molecularen_HK
dc.subject.meshCrystallins - geneticsen_HK
dc.subject.meshLens, crystalline - metabolismen_HK
dc.subject.meshMultigene familyen_HK
dc.titleGamma-crystallins of the human eye lens: expression analysis of five members of the gene family.en_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid3670288en_HK
dc.identifier.pmcidPMC367883-
dc.identifier.scopuseid_2-s2.0-0023389564en_HK
dc.identifier.volume7en_HK
dc.identifier.issue8en_HK
dc.identifier.spage2671en_HK
dc.identifier.epage2679en_HK
dc.identifier.isiWOS:A1987J391400003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMeakin, SO=7006735265en_HK
dc.identifier.scopusauthoridDu, RP=55204157500en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridBreitman, ML=7005448008en_HK

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