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Article: In situ detection of beta-galactosidase in lenses of transgenic mice with a gamma-crystallin/lacZ gene

TitleIn situ detection of beta-galactosidase in lenses of transgenic mice with a gamma-crystallin/lacZ gene
Authors
Issue Date1987
PublisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
Citation
Science, 1987, v. 235 n. 4787, p. 456-458 How to Cite?
AbstractTransgenic mice carrying the gamma 2-crystallin promoter fused to the coding region of the bacterial lacZ gene were generated. The offspring of three founder mice expressed high levels of the enzyme solely in the central nuclear fiber cells of the lens as measured by an in situ assay for the detection of beta-galactosidase activity. These results suggest that gamma 2-crystallin sequences between -759 to +45 contain essential information required for appropriate tissue-specific and temporal regulation of the mouse gamma 2-crystallin gene. In a broader context, this study also demonstrates the utility of beta-galactosidase hybrid gene constructs for monitoring the activity of gene regulatory elements in transgenic mice.
Persistent Identifierhttp://hdl.handle.net/10722/44221
ISSN
2015 Impact Factor: 34.661
2015 SCImago Journal Rankings: 13.217
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGoring, DRen_HK
dc.contributor.authorRossant, Jen_HK
dc.contributor.authorClapoff, Sen_HK
dc.contributor.authorBreitman, MLen_HK
dc.contributor.authorTsui, L-Cen_HK
dc.date.accessioned2007-09-12T03:49:17Z-
dc.date.available2007-09-12T03:49:17Z-
dc.date.issued1987en_HK
dc.identifier.citationScience, 1987, v. 235 n. 4787, p. 456-458en_HK
dc.identifier.issn0036-8075en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44221-
dc.description.abstractTransgenic mice carrying the gamma 2-crystallin promoter fused to the coding region of the bacterial lacZ gene were generated. The offspring of three founder mice expressed high levels of the enzyme solely in the central nuclear fiber cells of the lens as measured by an in situ assay for the detection of beta-galactosidase activity. These results suggest that gamma 2-crystallin sequences between -759 to +45 contain essential information required for appropriate tissue-specific and temporal regulation of the mouse gamma 2-crystallin gene. In a broader context, this study also demonstrates the utility of beta-galactosidase hybrid gene constructs for monitoring the activity of gene regulatory elements in transgenic mice.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.orgen_HK
dc.relation.ispartofScience-
dc.rightsScience. Copyright © American Association for the Advancement of Science.en_HK
dc.subject.meshCataract - enzymologyen_HK
dc.subject.meshCrystallins - geneticsen_HK
dc.subject.meshGalactosidases - geneticsen_HK
dc.subject.meshGene expression regulationen_HK
dc.subject.meshLens, crystalline - physiologyen_HK
dc.titleIn situ detection of beta-galactosidase in lenses of transgenic mice with a gamma-crystallin/lacZ geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0036-8075&volume=235&issue=4787&spage=456&epage=458&date=1987&atitle=In+situ+detection+of+b-galactosidase+in+lenses+of+transgenic+mice+with+a+g-crystallin/lacZ+geneen_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.pmid3099390-
dc.identifier.scopuseid_2-s2.0-0023141516-
dc.identifier.isiWOS:A1987F689100023-

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