File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Study of the expression of myelin proteolipid protein (lipophilin) using a cloned complementary DNA

TitleStudy of the expression of myelin proteolipid protein (lipophilin) using a cloned complementary DNA
Authors
Issue Date1985
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 1985, v. 13 n. 20, p. 7413-7425 How to Cite?
AbstractWe have prepared a λgt10 cDNA library with the mRNA isolated from fetal calf brains which were actively myelinating. Using two oligonucleotides made according to the known amino acid sequence of myelin proteolipid protein (PLP or lipophilin), we have isolated several cDNA clones for this major intrinsic membrane protein of myelin. One of these clones, designated as pLP1, is found to contain 444 bp of coding sequence for the C-terminal half of PLP and 486 bp of 3′ untranslated sequence. Using pLP1 as a hybridization probe, we have studied the regulation of PLP at the mRNA level during rat brain development. Our results show that the relative amounts of mRNA for PLP and that for the major extrinsic protein of the myelin membrane, myelin basic protein, increase coordinately during the course of myelination in the brain. © 1985 IRL Press Limited.
Persistent Identifierhttp://hdl.handle.net/10722/44213
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNaismith, ALen_HK
dc.contributor.authorHoffmanChudzik, Een_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorRiordan, JRen_HK
dc.date.accessioned2007-09-12T03:49:08Z-
dc.date.available2007-09-12T03:49:08Z-
dc.date.issued1985en_HK
dc.identifier.citationNucleic Acids Research, 1985, v. 13 n. 20, p. 7413-7425en_HK
dc.identifier.issn0305-1048en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44213-
dc.description.abstractWe have prepared a λgt10 cDNA library with the mRNA isolated from fetal calf brains which were actively myelinating. Using two oligonucleotides made according to the known amino acid sequence of myelin proteolipid protein (PLP or lipophilin), we have isolated several cDNA clones for this major intrinsic membrane protein of myelin. One of these clones, designated as pLP1, is found to contain 444 bp of coding sequence for the C-terminal half of PLP and 486 bp of 3′ untranslated sequence. Using pLP1 as a hybridization probe, we have studied the regulation of PLP at the mRNA level during rat brain development. Our results show that the relative amounts of mRNA for PLP and that for the major extrinsic protein of the myelin membrane, myelin basic protein, increase coordinately during the course of myelination in the brain. © 1985 IRL Press Limited.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_HK
dc.relation.ispartofNucleic Acids Researchen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAmino acid sequenceen_HK
dc.subject.meshBrain - embryology - growth & development - physiologyen_HK
dc.subject.meshDna - geneticsen_HK
dc.subject.meshGene expression regulationen_HK
dc.subject.meshMyelin proteins - geneticsen_HK
dc.titleStudy of the expression of myelin proteolipid protein (lipophilin) using a cloned complementary DNAen_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1093/nar/13.20.7413en_HK
dc.identifier.pmid3840591-
dc.identifier.pmcidPMC322052-
dc.identifier.scopuseid_2-s2.0-0022432487en_HK
dc.identifier.volume13en_HK
dc.identifier.issue20en_HK
dc.identifier.spage7413en_HK
dc.identifier.epage7425en_HK
dc.identifier.isiWOS:A1985ATH5700017-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridNaismith, AL=6602332581en_HK
dc.identifier.scopusauthoridHoffmanChudzik, E=6504330883en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridRiordan, JR=7202229758en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats