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Article: Lens-specific promoter activity of a mouse g-crystallin gene

TitleLens-specific promoter activity of a mouse g-crystallin gene
Authors
Issue Date1985
PublisherAmerican Society for Microbiology.
Citation
Molecular and cellular biology, 1985, v. 5 n. 9, p. 2221-2230 How to Cite?
AbstractCrystallins are the major water-soluble proteins in vertebrate eye lenses. These lens-specific proteins are encoded by several gene families, and their expression is differentially regulated during lens cell differentiation. Here we show that a cloned mouse gamma-crystallin promoter is active in lens explants derived from 14-day-old chicken embryos but inactive in a variety of cells of non-lens origin. We also show that sequences required for proper utilization of this promoter are contained between nucleotide positions -392 and +47 relative to the transcription initiation site; deletion of sequences from positions -392 to -171 completely abolishes promoter activity. Since chickens do not have gamma-crystallin genes, the expression of a mouse gamma-crystallin promoter in chicken lens cells suggests that different classes of crystallin genes may be regulated by common lens tissue-specific mechanism(s) independent of species.
Persistent Identifierhttp://hdl.handle.net/10722/44211
ISSN
2015 Impact Factor: 4.427
2015 SCImago Journal Rankings: 3.806
Other Identifiers
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLok, Sen_HK
dc.contributor.authorBreitman, MLen_HK
dc.contributor.authorChepelinsky, ABen_HK
dc.contributor.authorPiatigorsky, Jen_HK
dc.contributor.authorGold, RJMen_HK
dc.contributor.authorTsui, L-Cen_HK
dc.date.accessioned2007-09-12T03:49:06Z-
dc.date.available2007-09-12T03:49:06Z-
dc.date.issued1985en_HK
dc.identifierhttp://mcb.asm.org/cgi/reprint/5/9/2221.pdfen_HK
dc.identifier.citationMolecular and cellular biology, 1985, v. 5 n. 9, p. 2221-2230en_HK
dc.identifier.issn0270-7306en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44211-
dc.description.abstractCrystallins are the major water-soluble proteins in vertebrate eye lenses. These lens-specific proteins are encoded by several gene families, and their expression is differentially regulated during lens cell differentiation. Here we show that a cloned mouse gamma-crystallin promoter is active in lens explants derived from 14-day-old chicken embryos but inactive in a variety of cells of non-lens origin. We also show that sequences required for proper utilization of this promoter are contained between nucleotide positions -392 and +47 relative to the transcription initiation site; deletion of sequences from positions -392 to -171 completely abolishes promoter activity. Since chickens do not have gamma-crystallin genes, the expression of a mouse gamma-crystallin promoter in chicken lens cells suggests that different classes of crystallin genes may be regulated by common lens tissue-specific mechanism(s) independent of species.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Molecular and cellular biology, 1985, v. 5 n. 9, p. 2221-2230en_HK
dc.subject.meshBiology - biochemistry biology - cytology and histologyen_HK
dc.subject.meshChick embryoen_HK
dc.subject.meshCrystallins - biosynthesis - geneticsen_HK
dc.subject.meshDna, recombinant - metabolismen_HK
dc.subject.meshGene expression regulationen_HK
dc.titleLens-specific promoter activity of a mouse g-crystallin geneen_HK
dc.typeArticleen_HK
dc.description.naturelink_to_OA_fulltexten_HK
dc.identifier.pmid3837188-
dc.identifier.pmcidPMC366947-
dc.identifier.isiWOS:A1985AQH5200008-

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