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Article: Analysis of the mouse γ-crystallin gene family: Assignment of multiple cDNAs to discrete genomic sequences and characterization of a representative gene
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TitleAnalysis of the mouse γ-crystallin gene family: Assignment of multiple cDNAs to discrete genomic sequences and characterization of a representative gene
 
AuthorsLok, S2
Tsui, LC2 3
Shinohara, T1
Piatigorsky, J1
Gold, R2
Breitman, M2 3
 
Issue Date1984
 
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
 
CitationNucleic Acids Research, 1984, v. 12 n. 11, p. 4517-4529 [How to Cite?]
DOI: http://dx.doi.org/10.1093/nar/12.11.4517
 
AbstractBlot hybridization analysis of mouse DNA with γ-crystallin-specific CDNAs has detected the presence of a multigene family comprised of at least four related genes. The detailed structure of one of these genes, mouse γ 4-crystallin (Mγ 4.1), and its corresponding cDNA has been determined. The gene spans approximately 2.6 kilobases (kb) and contains two introns. The gene predicts a polypeptide of 174 amino acids that shares extensive sequence homology with γ-crystallin polypeptides of other species. The two similar structural domains of the protein correspond exactly to the second and third exons of the gene, supporting an exon-duplication model of gene evolution. The similarity in structure of this gene to that recently reported for a γ-crystallin gene of the rat (1) suggests that a common structure may exist for all γ-crystallin genes of the two species. Moreover, a highly conserved region, 50 nucleotides in length, immediately precedes the TATA box of both the mouse and rat genes, suggesting that this sequence may be important in gene regulation. © 1984 IRL Press Limited.
 
ISSN0305-1048
2013 Impact Factor: 8.808
 
DOIhttp://dx.doi.org/10.1093/nar/12.11.4517
 
PubMed Central IDPMC318855
 
ISI Accession Number IDWOS:A1984SW20600008
 
DC FieldValue
dc.contributor.authorLok, S
 
dc.contributor.authorTsui, LC
 
dc.contributor.authorShinohara, T
 
dc.contributor.authorPiatigorsky, J
 
dc.contributor.authorGold, R
 
dc.contributor.authorBreitman, M
 
dc.date.accessioned2007-09-12T03:49:00Z
 
dc.date.available2007-09-12T03:49:00Z
 
dc.date.issued1984
 
dc.description.abstractBlot hybridization analysis of mouse DNA with γ-crystallin-specific CDNAs has detected the presence of a multigene family comprised of at least four related genes. The detailed structure of one of these genes, mouse γ 4-crystallin (Mγ 4.1), and its corresponding cDNA has been determined. The gene spans approximately 2.6 kilobases (kb) and contains two introns. The gene predicts a polypeptide of 174 amino acids that shares extensive sequence homology with γ-crystallin polypeptides of other species. The two similar structural domains of the protein correspond exactly to the second and third exons of the gene, supporting an exon-duplication model of gene evolution. The similarity in structure of this gene to that recently reported for a γ-crystallin gene of the rat (1) suggests that a common structure may exist for all γ-crystallin genes of the two species. Moreover, a highly conserved region, 50 nucleotides in length, immediately precedes the TATA box of both the mouse and rat genes, suggesting that this sequence may be important in gene regulation. © 1984 IRL Press Limited.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationNucleic Acids Research, 1984, v. 12 n. 11, p. 4517-4529 [How to Cite?]
DOI: http://dx.doi.org/10.1093/nar/12.11.4517
 
dc.identifier.doihttp://dx.doi.org/10.1093/nar/12.11.4517
 
dc.identifier.epage4529
 
dc.identifier.isiWOS:A1984SW20600008
 
dc.identifier.issn0305-1048
2013 Impact Factor: 8.808
 
dc.identifier.issue11
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC318855
 
dc.identifier.pmid6330674
 
dc.identifier.scopuseid_2-s2.0-0021760456
 
dc.identifier.spage4517
 
dc.identifier.urihttp://hdl.handle.net/10722/44207
 
dc.identifier.volume12
 
dc.languageeng
 
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofNucleic Acids Research
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshAmino acid sequence
 
dc.subject.meshBacteriophage lambda - genetics
 
dc.subject.meshCloning, molecular
 
dc.subject.meshCrystallins - genetics
 
dc.subject.meshDna - analysis
 
dc.titleAnalysis of the mouse γ-crystallin gene family: Assignment of multiple cDNAs to discrete genomic sequences and characterization of a representative gene
 
dc.typeArticle
 
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<contributor.author>Tsui, LC</contributor.author>
<contributor.author>Shinohara, T</contributor.author>
<contributor.author>Piatigorsky, J</contributor.author>
<contributor.author>Gold, R</contributor.author>
<contributor.author>Breitman, M</contributor.author>
<date.accessioned>2007-09-12T03:49:00Z</date.accessioned>
<date.available>2007-09-12T03:49:00Z</date.available>
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<description.abstract>Blot hybridization analysis of mouse DNA with &#947;-crystallin-specific CDNAs has detected the presence of a multigene family comprised of at least four related genes. The detailed structure of one of these genes, mouse &#947; 4-crystallin (M&#947; 4.1), and its corresponding cDNA has been determined. The gene spans approximately 2.6 kilobases (kb) and contains two introns. The gene predicts a polypeptide of 174 amino acids that shares extensive sequence homology with &#947;-crystallin polypeptides of other species. The two similar structural domains of the protein correspond exactly to the second and third exons of the gene, supporting an exon-duplication model of gene evolution. The similarity in structure of this gene to that recently reported for a &#947;-crystallin gene of the rat (1) suggests that a common structure may exist for all &#947;-crystallin genes of the two species. Moreover, a highly conserved region, 50 nucleotides in length, immediately precedes the TATA box of both the mouse and rat genes, suggesting that this sequence may be important in gene regulation. &#169; 1984 IRL Press Limited.</description.abstract>
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<subject.mesh>Amino acid sequence</subject.mesh>
<subject.mesh>Bacteriophage lambda - genetics</subject.mesh>
<subject.mesh>Cloning, molecular</subject.mesh>
<subject.mesh>Crystallins - genetics</subject.mesh>
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Author Affiliations
  1. National Eye Institute
  2. University of Toronto
  3. Hospital for Sick Children University of Toronto