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Article: Role of gene T in phage λ tail assembly

TitleRole of gene T in phage λ tail assembly
Authors
Issue Date1983
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
Citation
Virology, 1983, v. 125 n. 2, p. 265-273 How to Cite?
AbstractWe have studied the phenotype of a heat-sensitive mutation that defines λ tail gene T. Induction and growth at the nonpermissive temperature of a λTts40 prophage results in production of morphologically normal but biologically inactive tails, which can be activated in vitro by lysates supplying the products of genes T, U, and Z. Thus, gene T acts between genes V and U in the tail assembly pathway or immediately after the completion of tail shaft polymerization. λTs40 lysates also contain a substantial number of morphologically normal phage particles which are deficient in cleavage of the minor tail protein gpH. This result is consistent with the fact that gpH cleavage normally occurs later in the assembly pathway than the time found for gene T action. Purified λTts40 virions that were produced at the permissive temperature are normal with respect to gpH cleavage. However, they are more sensitive to heat inactivation than are wild-type virions, suggesting that the T gene product is in the virion. At the permissive temperature, the mutant protein made by λTts40 is active in vivo even if it has been synthesized at the nonpermissive temperature.
Persistent Identifierhttp://hdl.handle.net/10722/44206
ISSN
2015 Impact Factor: 3.2
2015 SCImago Journal Rankings: 1.805
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorHendrix, RWen_HK
dc.date.accessioned2007-09-12T03:48:59Z-
dc.date.available2007-09-12T03:48:59Z-
dc.date.issued1983en_HK
dc.identifier.citationVirology, 1983, v. 125 n. 2, p. 265-273en_HK
dc.identifier.issn0042-6822en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44206-
dc.description.abstractWe have studied the phenotype of a heat-sensitive mutation that defines λ tail gene T. Induction and growth at the nonpermissive temperature of a λTts40 prophage results in production of morphologically normal but biologically inactive tails, which can be activated in vitro by lysates supplying the products of genes T, U, and Z. Thus, gene T acts between genes V and U in the tail assembly pathway or immediately after the completion of tail shaft polymerization. λTs40 lysates also contain a substantial number of morphologically normal phage particles which are deficient in cleavage of the minor tail protein gpH. This result is consistent with the fact that gpH cleavage normally occurs later in the assembly pathway than the time found for gene T action. Purified λTts40 virions that were produced at the permissive temperature are normal with respect to gpH cleavage. However, they are more sensitive to heat inactivation than are wild-type virions, suggesting that the T gene product is in the virion. At the permissive temperature, the mutant protein made by λTts40 is active in vivo even if it has been synthesized at the nonpermissive temperature.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviroen_HK
dc.relation.ispartofVirologyen_HK
dc.subject.meshBacteriophage lambda - geneticsen_HK
dc.subject.meshGenes, viralen_HK
dc.subject.meshGenetic complementation testen_HK
dc.subject.meshMutationen_HK
dc.subject.meshViral proteins - metabolismen_HK
dc.titleRole of gene T in phage λ tail assemblyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0042-6822&volume=125&issue=2&spage=265&epage=273&date=1983&atitle=Role+of+gene+T+in+phage+lambda+tail+assemblyen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1016/0042-6822(83)90200-3-
dc.identifier.pmid6220514-
dc.identifier.scopuseid_2-s2.0-0020522445en_HK
dc.identifier.volume125en_HK
dc.identifier.issue2en_HK
dc.identifier.spage265en_HK
dc.identifier.epage273en_HK
dc.identifier.isiWOS:A1983QJ42000002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridHendrix, RW=7005953845en_HK

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