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Article: Persistence of freely replicating SV40 recombinant molecules carrying a selectable marker in permissive simian cells

TitlePersistence of freely replicating SV40 recombinant molecules carrying a selectable marker in permissive simian cells
Authors
Issue Date1982
PublisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/cell
Citation
Cell, 1982, v. 30 n. 2, p. 499-508 How to Cite?
AbstractWe have demonstrated that a SV40-pBR322 recombinant vector (pSV2-gpt) carrying a bacterial gene of selectable phenotype (Eco-gpt) may persist extrachromosomally in COS1 cells, a simian cell line that endogenously produces SV40 large T antigen. The amount of circular (supercoiled) recombinant DNA was estimated to be between 5 and 2000 copies per cell among several pSV2-transformed COS1 clonal lines examined. Complete pSV2 molecules were found in the majority of the transformants, although some of the pSV2 DNAs recovered were shown to have deletions in the pBR322 region. Our results indicate that removal of the pBR322 "inhibitory sequence" in pSV2 is not necessary for stable maintenance of these recombinant molecules in COS1 cells. In addition, large amounts of pSV2-related high molecular weight DNAs, probably concatemers of pSV2, were detected in the transformed lines. © 1982.
Persistent Identifierhttp://hdl.handle.net/10722/44204
ISSN
2015 Impact Factor: 28.71
2015 SCImago Journal Rankings: 28.188
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorBreitman, MLen_HK
dc.contributor.authorSiminovitch, Len_HK
dc.contributor.authorBuchwald, Men_HK
dc.date.accessioned2007-09-12T03:48:57Z-
dc.date.available2007-09-12T03:48:57Z-
dc.date.issued1982en_HK
dc.identifier.citationCell, 1982, v. 30 n. 2, p. 499-508en_HK
dc.identifier.issn0092-8674en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44204-
dc.description.abstractWe have demonstrated that a SV40-pBR322 recombinant vector (pSV2-gpt) carrying a bacterial gene of selectable phenotype (Eco-gpt) may persist extrachromosomally in COS1 cells, a simian cell line that endogenously produces SV40 large T antigen. The amount of circular (supercoiled) recombinant DNA was estimated to be between 5 and 2000 copies per cell among several pSV2-transformed COS1 clonal lines examined. Complete pSV2 molecules were found in the majority of the transformants, although some of the pSV2 DNAs recovered were shown to have deletions in the pBR322 region. Our results indicate that removal of the pBR322 "inhibitory sequence" in pSV2 is not necessary for stable maintenance of these recombinant molecules in COS1 cells. In addition, large amounts of pSV2-related high molecular weight DNAs, probably concatemers of pSV2, were detected in the transformed lines. © 1982.en_HK
dc.languageengen_HK
dc.publisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/cellen_HK
dc.relation.ispartofCellen_HK
dc.rightsThis paper has been submitted to Cancer Cell for considerationen_HK
dc.subject.meshAntigens, viralen_HK
dc.subject.meshAntigens, viral, tumoren_HK
dc.subject.meshCell transformation, viralen_HK
dc.subject.meshDna replicationen_HK
dc.subject.meshDna, recombinant - biosynthesisen_HK
dc.titlePersistence of freely replicating SV40 recombinant molecules carrying a selectable marker in permissive simian cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0092-8674&volume=30&issue=2&spage=499&epage=508&date=1982&atitle=Persistence+of+a+freely+replicating+SV40+recombinant+molecule+carrying+a+selectable+marker+in+permissive+simian+cellsen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1016/0092-8674(82)90247-1en_HK
dc.identifier.pmid6291772-
dc.identifier.scopuseid_2-s2.0-0020181589en_HK
dc.identifier.volume30en_HK
dc.identifier.issue2en_HK
dc.identifier.spage499en_HK
dc.identifier.epage508en_HK
dc.identifier.isiWOS:A1982PH54600017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridBreitman, ML=7005448008en_HK
dc.identifier.scopusauthoridSiminovitch, L=7006464595en_HK
dc.identifier.scopusauthoridBuchwald, M=7006759922en_HK

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