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Article: Head-tail connector of bacteriophage lambda

TitleHead-tail connector of bacteriophage lambda
Authors
Issue Date1980
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/jmb
Citation
Journal Of Molecular Biology, 1980, v. 142 n. 3, p. 419-438 How to Cite?
AbstractThe head-tail connector of phage λ, a protein knob inside the head shell to which the tail attaches, is composed primarily of head protein gpB § and its cleaved form gpB*. All of the gpB and gpB* in the virion is located in the connector. gpFII, the protein that is thought to form the site on the head to which the tail binds, is also located in the connector. Head proteins gpE, gpD, X1 and X2 are not components of the connector. These assignments were made by disrupting virions with guanidine hydrochloride, in such a way that heads and tails separate with the connectors attached to the tails, and determining which head proteins co-purify with the tails. We find that lysates from a λE− infection contain a high proportion of tails with connectors attached. (Gene E codes for the major component of the head shell.) Connectors are also present on tails from a λE−C− infection, arguing that gpE, gpC, and their processed forms, X1 and X2, are all unnecessary for assembly of biologically competent connectors. The gpB in the connectors on E− and E−C− tails is in the uncleaved form. Connectors are not seen on tails from infections by λE−B−, λE−FII−, or λE− in a groE− host.
Persistent Identifierhttp://hdl.handle.net/10722/44202
ISSN
2015 Impact Factor: 4.517
2015 SCImago Journal Rankings: 3.002
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTsui, Len_HK
dc.contributor.authorHendrix, RWen_HK
dc.date.accessioned2007-09-12T03:48:55Z-
dc.date.available2007-09-12T03:48:55Z-
dc.date.issued1980en_HK
dc.identifier.citationJournal Of Molecular Biology, 1980, v. 142 n. 3, p. 419-438en_HK
dc.identifier.issn0022-2836en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44202-
dc.description.abstractThe head-tail connector of phage λ, a protein knob inside the head shell to which the tail attaches, is composed primarily of head protein gpB § and its cleaved form gpB*. All of the gpB and gpB* in the virion is located in the connector. gpFII, the protein that is thought to form the site on the head to which the tail binds, is also located in the connector. Head proteins gpE, gpD, X1 and X2 are not components of the connector. These assignments were made by disrupting virions with guanidine hydrochloride, in such a way that heads and tails separate with the connectors attached to the tails, and determining which head proteins co-purify with the tails. We find that lysates from a λE− infection contain a high proportion of tails with connectors attached. (Gene E codes for the major component of the head shell.) Connectors are also present on tails from a λE−C− infection, arguing that gpE, gpC, and their processed forms, X1 and X2, are all unnecessary for assembly of biologically competent connectors. The gpB in the connectors on E− and E−C− tails is in the uncleaved form. Connectors are not seen on tails from infections by λE−B−, λE−FII−, or λE− in a groE− host.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/jmben_HK
dc.relation.ispartofJournal of Molecular Biologyen_HK
dc.subject.meshBacteriophage lambda - analysis - growth & development - ultrastructureen_HK
dc.subject.meshCentrifugation, density gradienten_HK
dc.subject.meshElectrophoresis, polyacrylamide gelen_HK
dc.subject.meshGuanidinesen_HK
dc.subject.meshMicroscopy, electronen_HK
dc.titleHead-tail connector of bacteriophage lambdaen_HK
dc.typeArticleen_HK
dc.identifier.emailTsui, L: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, L=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1016/0022-2836(80)90280-6en_HK
dc.identifier.pmid6450838-
dc.identifier.scopuseid_2-s2.0-0019277120en_HK
dc.identifier.volume142en_HK
dc.identifier.issue3en_HK
dc.identifier.spage419en_HK
dc.identifier.epage438en_HK
dc.identifier.isiWOS:A1980KJ72300008-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTsui, L=7102754167en_HK
dc.identifier.scopusauthoridHendrix, RW=7005953845en_HK

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