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Article: Role of the host in virus assembly: Cloning of the Escherichia coli groE gene and identification of its protein product

TitleRole of the host in virus assembly: Cloning of the Escherichia coli groE gene and identification of its protein product
Authors
Issue Date1978
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings Of The National Academy Of Sciences Of The United States Of America, 1978, v. 75 n. 1, p. 136-139 How to Cite?
AbstractCorrect assembly of the heads of bacteriophages λ and T4 requires the function of the groE gene of the Escherichia coli host. We have isolated a transducing derivative of λ, called λgt-Ec.groE, that carries a functional copy of the groE gene. Unlike wild-type λ, this phage is able to form plaques on hosts with a mutant groE gene. We have isolated an amber mutation in the groE gene carried by the phage, and this has made it possible to identify the groE product as a protein of molecular weight 65,000. In the phage, the groE gene is under the control of an early phage promoter.
Persistent Identifierhttp://hdl.handle.net/10722/44201
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHendrix, RWen_HK
dc.contributor.authorTsui, Len_HK
dc.date.accessioned2007-09-12T03:48:54Z-
dc.date.available2007-09-12T03:48:54Z-
dc.date.issued1978en_HK
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America, 1978, v. 75 n. 1, p. 136-139en_HK
dc.identifier.issn0027-8424en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44201-
dc.description.abstractCorrect assembly of the heads of bacteriophages λ and T4 requires the function of the groE gene of the Escherichia coli host. We have isolated a transducing derivative of λ, called λgt-Ec.groE, that carries a functional copy of the groE gene. Unlike wild-type λ, this phage is able to form plaques on hosts with a mutant groE gene. We have isolated an amber mutation in the groE gene carried by the phage, and this has made it possible to identify the groE product as a protein of molecular weight 65,000. In the phage, the groE gene is under the control of an early phage promoter.en_HK
dc.languageengen_HK
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_HK
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_HK
dc.subject.meshBacterial proteins - geneticsen_HK
dc.subject.meshColiphages - genetics - metabolismen_HK
dc.subject.meshDna, recombinanten_HK
dc.subject.meshEscherichia coli - geneticsen_HK
dc.subject.meshMolecular weighten_HK
dc.titleRole of the host in virus assembly: Cloning of the Escherichia coli groE gene and identification of its protein producten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0027-8424&volume=75&issue=1&spage=136&epage=139&date=1978&atitle=Role+of+the+host+in+virus+assembly:+Cloning+of+the+Escherichia+coli+groE+gene+and+identification+of+its+protein+producten_HK
dc.identifier.emailTsui, L: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, L=rp00058en_HK
dc.description.naturelink_to_OA_fulltexten_HK
dc.identifier.pmid343102-
dc.identifier.pmcidPMC411199-
dc.identifier.scopuseid_2-s2.0-0017813211en_HK
dc.identifier.volume75en_HK
dc.identifier.issue1en_HK
dc.identifier.spage136en_HK
dc.identifier.epage139en_HK
dc.identifier.isiWOS:A1978EM12800031-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHendrix, RW=7005953845en_HK
dc.identifier.scopusauthoridTsui, L=7102754167en_HK

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