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- Publisher Website: 10.1007/BF00269403
- Scopus: eid_2-s2.0-0017257530
- PMID: 765760
- WOS: WOS:A1976BF45800005
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Article: The depression of endolysin synthesis in bacteria infected with high multiplicities of phage λ
Title | The depression of endolysin synthesis in bacteria infected with high multiplicities of phage λ |
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Authors | |
Issue Date | 1976 |
Publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00438/index.htm |
Citation | Molecular And General Genetics, 1976, v. 143 n. 3, p. 269-278 How to Cite? |
Abstract | The effect of multiplicity of infection was studied in Escherichia coli with λ phage, using phage endolysin as an example of a late gene product. A very sensitive endolysin assay method was used so that the initiation time of endolysin synthesis could be more accurately determined. It was observed that high multiplicity of infection increases the rate of lysogenization, progressively delays lysis time, and significantly delays and reduces the synthesis of endolysin in λcIII+ cII+ infected cells. The extent of delay and reduction in endolysin synthesis increases with increasing multiplicity. In contrast, λcIII67cII68 infected cells show no delay in endolysin synthesis at high multiplicity of infection when compared with the λcIII+ cII+ infected cells. The results suggest that the expression of cIII and cII genes is multiplicity dependent, high multiplicity of infection enhances the expression of the cIII and cII genes, and the expression of the cIII and cII genes interferes with the expression of the late genes. A model to explain how the expression of the cIII and cII genes interferes with the expression of the late genes is proposed. |
Persistent Identifier | http://hdl.handle.net/10722/44200 |
ISSN | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Tsui, L | en_HK |
dc.contributor.author | Mark, K | en_HK |
dc.date.accessioned | 2007-09-12T03:48:52Z | - |
dc.date.available | 2007-09-12T03:48:52Z | - |
dc.date.issued | 1976 | en_HK |
dc.identifier.citation | Molecular And General Genetics, 1976, v. 143 n. 3, p. 269-278 | en_HK |
dc.identifier.issn | 0026-8925 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/44200 | - |
dc.description.abstract | The effect of multiplicity of infection was studied in Escherichia coli with λ phage, using phage endolysin as an example of a late gene product. A very sensitive endolysin assay method was used so that the initiation time of endolysin synthesis could be more accurately determined. It was observed that high multiplicity of infection increases the rate of lysogenization, progressively delays lysis time, and significantly delays and reduces the synthesis of endolysin in λcIII+ cII+ infected cells. The extent of delay and reduction in endolysin synthesis increases with increasing multiplicity. In contrast, λcIII67cII68 infected cells show no delay in endolysin synthesis at high multiplicity of infection when compared with the λcIII+ cII+ infected cells. The results suggest that the expression of cIII and cII genes is multiplicity dependent, high multiplicity of infection enhances the expression of the cIII and cII genes, and the expression of the cIII and cII genes interferes with the expression of the late genes. A model to explain how the expression of the cIII and cII genes interferes with the expression of the late genes is proposed. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00438/index.htm | en_HK |
dc.relation.ispartof | Molecular and General Genetics | en_HK |
dc.rights | The original publication is available at www.springerlink.com | en_HK |
dc.subject.mesh | Bacteriolysis | en_HK |
dc.subject.mesh | Coliphages - enzymology - metabolism | en_HK |
dc.subject.mesh | Endopeptidases - biosynthesis | en_HK |
dc.subject.mesh | Escherichia coli - enzymology | en_HK |
dc.subject.mesh | Galactosidases - metabolism | en_HK |
dc.title | The depression of endolysin synthesis in bacteria infected with high multiplicities of phage λ | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0026-8925&volume=143&issue=3&spage=269&epage=278&date=1976&atitle=The+depression+of+endolysin+synthesis+in+bacteria+infected+with+high+multiplicity+of+phage+lambda | en_HK |
dc.identifier.email | Tsui, L: tsuilc@hkucc.hku.hk | en_HK |
dc.identifier.authority | Tsui, L=rp00058 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | en_HK |
dc.identifier.doi | 10.1007/BF00269403 | en_HK |
dc.identifier.pmid | 765760 | en_HK |
dc.identifier.scopus | eid_2-s2.0-0017257530 | en_HK |
dc.identifier.volume | 143 | en_HK |
dc.identifier.issue | 3 | en_HK |
dc.identifier.spage | 269 | en_HK |
dc.identifier.epage | 278 | en_HK |
dc.identifier.isi | WOS:A1976BF45800005 | - |
dc.publisher.place | Germany | en_HK |
dc.identifier.scopusauthorid | Tsui, L=7102754167 | en_HK |
dc.identifier.scopusauthorid | Mark, K=7006784243 | en_HK |
dc.identifier.issnl | 0026-8925 | - |