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Article: The depression of endolysin synthesis in bacteria infected with high multiplicities of phage λ

TitleThe depression of endolysin synthesis in bacteria infected with high multiplicities of phage λ
Authors
Issue Date1976
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00438/index.htm
Citation
Molecular And General Genetics, 1976, v. 143 n. 3, p. 269-278 How to Cite?
AbstractThe effect of multiplicity of infection was studied in Escherichia coli with λ phage, using phage endolysin as an example of a late gene product. A very sensitive endolysin assay method was used so that the initiation time of endolysin synthesis could be more accurately determined. It was observed that high multiplicity of infection increases the rate of lysogenization, progressively delays lysis time, and significantly delays and reduces the synthesis of endolysin in λcIII+ cII+ infected cells. The extent of delay and reduction in endolysin synthesis increases with increasing multiplicity. In contrast, λcIII67cII68 infected cells show no delay in endolysin synthesis at high multiplicity of infection when compared with the λcIII+ cII+ infected cells. The results suggest that the expression of cIII and cII genes is multiplicity dependent, high multiplicity of infection enhances the expression of the cIII and cII genes, and the expression of the cIII and cII genes interferes with the expression of the late genes. A model to explain how the expression of the cIII and cII genes interferes with the expression of the late genes is proposed.
Persistent Identifierhttp://hdl.handle.net/10722/44200
ISSN
2003 Impact Factor: 2.727
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTsui, Len_HK
dc.contributor.authorMark, Ken_HK
dc.date.accessioned2007-09-12T03:48:52Z-
dc.date.available2007-09-12T03:48:52Z-
dc.date.issued1976en_HK
dc.identifier.citationMolecular And General Genetics, 1976, v. 143 n. 3, p. 269-278en_HK
dc.identifier.issn0026-8925en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44200-
dc.description.abstractThe effect of multiplicity of infection was studied in Escherichia coli with λ phage, using phage endolysin as an example of a late gene product. A very sensitive endolysin assay method was used so that the initiation time of endolysin synthesis could be more accurately determined. It was observed that high multiplicity of infection increases the rate of lysogenization, progressively delays lysis time, and significantly delays and reduces the synthesis of endolysin in λcIII+ cII+ infected cells. The extent of delay and reduction in endolysin synthesis increases with increasing multiplicity. In contrast, λcIII67cII68 infected cells show no delay in endolysin synthesis at high multiplicity of infection when compared with the λcIII+ cII+ infected cells. The results suggest that the expression of cIII and cII genes is multiplicity dependent, high multiplicity of infection enhances the expression of the cIII and cII genes, and the expression of the cIII and cII genes interferes with the expression of the late genes. A model to explain how the expression of the cIII and cII genes interferes with the expression of the late genes is proposed.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00438/index.htmen_HK
dc.relation.ispartofMolecular and General Geneticsen_HK
dc.rightsThe original publication is available at www.springerlink.comen_HK
dc.subject.meshBacteriolysisen_HK
dc.subject.meshColiphages - enzymology - metabolismen_HK
dc.subject.meshEndopeptidases - biosynthesisen_HK
dc.subject.meshEscherichia coli - enzymologyen_HK
dc.subject.meshGalactosidases - metabolismen_HK
dc.titleThe depression of endolysin synthesis in bacteria infected with high multiplicities of phage λen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0026-8925&volume=143&issue=3&spage=269&epage=278&date=1976&atitle=The+depression+of+endolysin+synthesis+in+bacteria+infected+with+high+multiplicity+of+phage+lambdaen_HK
dc.identifier.emailTsui, L: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, L=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltexten_HK
dc.identifier.doi10.1007/BF00269403en_HK
dc.identifier.pmid765760en_HK
dc.identifier.scopuseid_2-s2.0-0017257530en_HK
dc.identifier.volume143en_HK
dc.identifier.issue3en_HK
dc.identifier.spage269en_HK
dc.identifier.epage278en_HK
dc.identifier.isiWOS:A1976BF45800005-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridTsui, L=7102754167en_HK
dc.identifier.scopusauthoridMark, K=7006784243en_HK

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