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Article: Comparison of haemoglobin H inclusion bodies with embryonic ζ globin in screening for α thalassaemia

TitleComparison of haemoglobin H inclusion bodies with embryonic ζ globin in screening for α thalassaemia
Authors
Keywordsα thalassaemia
ζ chains
HbH inclusion test
Issue Date1995
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
Citation
Journal Of Clinical Pathology, 1995, v. 48 n. 9, p. 861-864 How to Cite?
AbstractAims - To compare the haemoglobin (Hb) H inclusion test with immunocytochemical detection of embryonic ζ chains in screening for a thalassaemia. Methods - Blood samples from 115 patients with relevant clinical history and hypochromic microcytic indexes were screened using the HbH inclusion test and the Variant Hemoglobin Testing System (BioRad, Hercules, CA, USA). Results - The HbH inclusion test was positive in 61 of 115 cases, three of whom had HbH disease confirmed by electrophoresis. The remaining 58 had α thalassaemia 1. All three HbH cases and 56 of 58 cases of a thalassaemia 1 expressed embryonic ζ chains, giving a specificity of 96.7%. Fifty four of 115 cases had a negative HbH inclusion test, of whom 50 had β thalassaemia trait and three had iron deficiency. No diagnosis was reached for the remaining patient. Conclusion - The immunocytochemical test is as sensitive as the HbH inclusion test in screening for a thalassaemia. The presence of ζ chains is highly specific for a thalassaemia I incorporating the (--/SEA) deletion. The specificity and simplicity of the immunocytochemical test make it the test of choice in screening for α thalassaemia.
Persistent Identifierhttp://hdl.handle.net/10722/43614
ISSN
2015 Impact Factor: 2.912
2015 SCImago Journal Rankings: 1.260
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, LCen_HK
dc.contributor.authorSo, JCCen_HK
dc.contributor.authorChui, DHKen_HK
dc.date.accessioned2007-03-23T04:50:21Z-
dc.date.available2007-03-23T04:50:21Z-
dc.date.issued1995en_HK
dc.identifier.citationJournal Of Clinical Pathology, 1995, v. 48 n. 9, p. 861-864en_HK
dc.identifier.issn0021-9746en_HK
dc.identifier.urihttp://hdl.handle.net/10722/43614-
dc.description.abstractAims - To compare the haemoglobin (Hb) H inclusion test with immunocytochemical detection of embryonic ζ chains in screening for a thalassaemia. Methods - Blood samples from 115 patients with relevant clinical history and hypochromic microcytic indexes were screened using the HbH inclusion test and the Variant Hemoglobin Testing System (BioRad, Hercules, CA, USA). Results - The HbH inclusion test was positive in 61 of 115 cases, three of whom had HbH disease confirmed by electrophoresis. The remaining 58 had α thalassaemia 1. All three HbH cases and 56 of 58 cases of a thalassaemia 1 expressed embryonic ζ chains, giving a specificity of 96.7%. Fifty four of 115 cases had a negative HbH inclusion test, of whom 50 had β thalassaemia trait and three had iron deficiency. No diagnosis was reached for the remaining patient. Conclusion - The immunocytochemical test is as sensitive as the HbH inclusion test in screening for a thalassaemia. The presence of ζ chains is highly specific for a thalassaemia I incorporating the (--/SEA) deletion. The specificity and simplicity of the immunocytochemical test make it the test of choice in screening for α thalassaemia.en_HK
dc.format.extent1102840 bytes-
dc.format.extent26624 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.en_HK
dc.subjectα thalassaemiaen_HK
dc.subjectζ chainsen_HK
dc.subjectHbH inclusion testen_HK
dc.subject.meshErythrocytes - ultrastructureen_HK
dc.subject.meshFluorescent antibody techniqueen_HK
dc.subject.meshGlobins - analysisen_HK
dc.subject.meshInclusion bodies - pathologyen_HK
dc.subject.meshAlpha-thalassemia - blood - diagnosis - geneticsen_HK
dc.titleComparison of haemoglobin H inclusion bodies with embryonic ζ globin in screening for α thalassaemiaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=48&issue=9&spage=861&epage=864&date=1995&atitle=Comparison+of+haemoglobin+H+inclusion+bodies+with+embryonic+zeta+globin+in+screening+for+alpha+thalassaemiaen_HK
dc.identifier.emailChan, LC:chanlc@hkucc.hku.hken_HK
dc.identifier.emailSo, JCC:scc@pathology.hku.hken_HK
dc.identifier.authorityChan, LC=rp00373en_HK
dc.identifier.authoritySo, JCC=rp00391en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid7490322-
dc.identifier.pmcidPMC502877-
dc.identifier.scopuseid_2-s2.0-0029091409en_HK
dc.identifier.hkuros8743-
dc.identifier.volume48en_HK
dc.identifier.issue9en_HK
dc.identifier.spage861en_HK
dc.identifier.epage864en_HK
dc.identifier.isiWOS:A1995RU00800015-
dc.publisher.placeUnited Kingdomen_HK

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