File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Activity of the EBNA1 promoter associated with lytic replication (Fp) in Epstein-Barr virus associated disorders

TitleActivity of the EBNA1 promoter associated with lytic replication (Fp) in Epstein-Barr virus associated disorders
Authors
KeywordsBamHI-F region
Epstein-Barr virus
Epstein-Barr virus nuclear antigen 1
Issue Date2001
PublisherB M J Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/
Citation
Journal Of Clinical Pathology - Molecular Pathology, 2001, v. 54 n. 2, p. 98-102 How to Cite?
AbstractBackground/Aims - In Epstein-Barr virus (EBV) positive cell lines that are stably infected, three different promoters are known to direct the transcription of EBV nuclear antigen 1 (EBNA1). These are located in the BamHI-C, BamHI-Q, and BamHI-F regions of the viral genome (Cp, Qp, and Fp, respectively). Fp is activated upon induction of the viral lytic cycle. The aim of this study was to investigate the activity of Fp in EBV associated diseases. Methods - Using reverse transcriptase polymerase chain reaction, a qualitative analysis of EBNA1 promoter usage in various EBV associated diseases was performed. Results - Fp driven transcription was detected in the context of primary infection and/or lytic replication; at least a portion of the Fp driven transcripts encoded EBNA1. Qp driven EBNA1 transcripts were detected in most samples across the range of disorders tested. Cp driven EBNA1 transcripts were detected in the context of immune suppression and in samples containing EBV positive (nonneoplastic) lymphoid cells. Conclusions - These results confirm the previously proposed housekeeping function of the Qp promoter.
Persistent Identifierhttp://hdl.handle.net/10722/43611
ISSN
2005 Impact Factor: 2.576
2005 SCImago Journal Rankings: 0.122
PubMed Central ID
References

 

DC FieldValueLanguage
dc.contributor.authorBrink, AATPen_HK
dc.contributor.authorMeijer, CJLMen_HK
dc.contributor.authorNicholls, JMen_HK
dc.contributor.authorMiddeldorp, JMen_HK
dc.contributor.authorVan den Brule, AJCen_HK
dc.date.accessioned2007-03-23T04:50:18Z-
dc.date.available2007-03-23T04:50:18Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Clinical Pathology - Molecular Pathology, 2001, v. 54 n. 2, p. 98-102en_HK
dc.identifier.issn1366-8714en_HK
dc.identifier.urihttp://hdl.handle.net/10722/43611-
dc.description.abstractBackground/Aims - In Epstein-Barr virus (EBV) positive cell lines that are stably infected, three different promoters are known to direct the transcription of EBV nuclear antigen 1 (EBNA1). These are located in the BamHI-C, BamHI-Q, and BamHI-F regions of the viral genome (Cp, Qp, and Fp, respectively). Fp is activated upon induction of the viral lytic cycle. The aim of this study was to investigate the activity of Fp in EBV associated diseases. Methods - Using reverse transcriptase polymerase chain reaction, a qualitative analysis of EBNA1 promoter usage in various EBV associated diseases was performed. Results - Fp driven transcription was detected in the context of primary infection and/or lytic replication; at least a portion of the Fp driven transcripts encoded EBNA1. Qp driven EBNA1 transcripts were detected in most samples across the range of disorders tested. Cp driven EBNA1 transcripts were detected in the context of immune suppression and in samples containing EBV positive (nonneoplastic) lymphoid cells. Conclusions - These results confirm the previously proposed housekeeping function of the Qp promoter.en_HK
dc.format.extent216283 bytes-
dc.format.extent25088 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathology - Molecular Pathologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsMolecular Pathology. Copyright © B M J Publishing Group.en_HK
dc.subjectBamHI-F regionen_HK
dc.subjectEpstein-Barr virusen_HK
dc.subjectEpstein-Barr virus nuclear antigen 1en_HK
dc.subject.meshDeoxyribonuclease bamhi - geneticsen_HK
dc.subject.meshEpstein-barr virus infections - geneticsen_HK
dc.subject.meshEpstein-barr virus nuclear antigens - geneticsen_HK
dc.subject.meshLymphoma - genetics - virologyen_HK
dc.subject.meshPromoter regions (genetics)en_HK
dc.titleActivity of the EBNA1 promoter associated with lytic replication (Fp) in Epstein-Barr virus associated disordersen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1366-8714&volume=54&issue=2&spage=98&epage=102&date=2001&atitle=Activity+of+the+EBNA1+promoter+associated+with+lytic+replication+(Fp)+in+Epstein-Barr+virus+associated+disordersen_HK
dc.identifier.emailNicholls, JM:nicholls@pathology.hku.hken_HK
dc.identifier.authorityNicholls, JM=rp00364en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1136/mp.54.2.98en_HK
dc.identifier.pmid11322171-
dc.identifier.pmcidPMC1187010-
dc.identifier.scopuseid_2-s2.0-0035061653en_HK
dc.identifier.hkuros56563-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035061653&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume54en_HK
dc.identifier.issue2en_HK
dc.identifier.spage98en_HK
dc.identifier.epage102en_HK
dc.publisher.placeUnited Kingdomen_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats