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Article: Lack of correlation between expression of Epstein-Barr virus (EBV) latent membrane protein and bcl-2 oncoprotein in vivo

TitleLack of correlation between expression of Epstein-Barr virus (EBV) latent membrane protein and bcl-2 oncoprotein in vivo
Authors
Issue Date1994
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
Citation
Journal Of Clinical Pathology, 1994, v. 47 n. 7, p. 589-591 How to Cite?
AbstractAims - To evaluate whether there is any correlation between the expression of Epstein-Barr virus (EBV) latent membrane protein (LMP) and oncoprotein bcl-2 in the lymph node biopsy specimens of a Chinese patient with EBV-related reactive lymphoproliferation who later developed T cell lymphoma after a short period of time. Methods - Immunohistochemistry, with a standard alkaline phosphatase anti-alkaline phosphatase (APAAP) method and New Fuchsin as a chromogen, was used for single staining of bcl-2 or LMP. Double immunostaining combining APAAP and indirect immunofluorescence was performed for dual labelling of LMP and bcl-2. Results - bcl-2 was expressed in 10-30% of cells in the first lymph node biopsy specimen (EBV-associated lymphoproliferative disorder) and 30-50% of cells in the second lymph node biopsy specimen (T cell lymphoma). LMP was expressed in the first biopsy specimen but not in the second. Double immunostaining results showed that around 78% of LMP positive cells were bcl-2 negative and 94% bcl-2 positive cells were LMP negative. Among the very small fraction of LMP and bcl-2 double positive cells, the intensity of bcl-2 staining was heterogeneous and was not always stronger than that observed in LMP negative bcl-2 positive cells. Conclusions - The expression of bcl-2 protein is independent of LMP protein status in vivo. Several mechanisms may be involved in EBV associated lymphomagenesis, and bcl-2 induction may occur independently of LMP expression.
Persistent Identifierhttp://hdl.handle.net/10722/43609
ISSN
2015 Impact Factor: 2.912
2015 SCImago Journal Rankings: 1.260
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTao, Qen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorLoke, SLen_HK
dc.contributor.authorHo, FCSen_HK
dc.date.accessioned2007-03-23T04:50:15Z-
dc.date.available2007-03-23T04:50:15Z-
dc.date.issued1994en_HK
dc.identifier.citationJournal Of Clinical Pathology, 1994, v. 47 n. 7, p. 589-591en_HK
dc.identifier.issn0021-9746en_HK
dc.identifier.urihttp://hdl.handle.net/10722/43609-
dc.description.abstractAims - To evaluate whether there is any correlation between the expression of Epstein-Barr virus (EBV) latent membrane protein (LMP) and oncoprotein bcl-2 in the lymph node biopsy specimens of a Chinese patient with EBV-related reactive lymphoproliferation who later developed T cell lymphoma after a short period of time. Methods - Immunohistochemistry, with a standard alkaline phosphatase anti-alkaline phosphatase (APAAP) method and New Fuchsin as a chromogen, was used for single staining of bcl-2 or LMP. Double immunostaining combining APAAP and indirect immunofluorescence was performed for dual labelling of LMP and bcl-2. Results - bcl-2 was expressed in 10-30% of cells in the first lymph node biopsy specimen (EBV-associated lymphoproliferative disorder) and 30-50% of cells in the second lymph node biopsy specimen (T cell lymphoma). LMP was expressed in the first biopsy specimen but not in the second. Double immunostaining results showed that around 78% of LMP positive cells were bcl-2 negative and 94% bcl-2 positive cells were LMP negative. Among the very small fraction of LMP and bcl-2 double positive cells, the intensity of bcl-2 staining was heterogeneous and was not always stronger than that observed in LMP negative bcl-2 positive cells. Conclusions - The expression of bcl-2 protein is independent of LMP protein status in vivo. Several mechanisms may be involved in EBV associated lymphomagenesis, and bcl-2 induction may occur independently of LMP expression.en_HK
dc.format.extent817879 bytes-
dc.format.extent25088 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.en_HK
dc.subject.meshFluorescent antibody techniqueen_HK
dc.subject.meshHerpesviridae infectionsen_HK
dc.subject.meshHerpesvirus 4, humanen_HK
dc.subject.meshLymphoma, t-cellen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.titleLack of correlation between expression of Epstein-Barr virus (EBV) latent membrane protein and bcl-2 oncoprotein in vivoen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=47&issue=7&spage=589&epage=591&date=1994&atitle=Lack+of+correlation+between+expression+of+Epstein-Barr+virus+(EBV)+latent+membrane+protein+and+bcl-2+oncoprotein+in+vivoen_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid8089211-
dc.identifier.pmcidPMC502067-
dc.identifier.scopuseid_2-s2.0-0028641566en_HK
dc.identifier.hkuros5246-
dc.identifier.volume47en_HK
dc.identifier.issue7en_HK
dc.identifier.spage589en_HK
dc.identifier.epage591en_HK
dc.identifier.isiWOS:A1994NW73700004-
dc.publisher.placeUnited Kingdomen_HK

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