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Article: Improved double immunohistochemical staining method for cryostat and paraffin wax sections, combining alkaline phosphatase anti-alkaline phosphatase and indirect immunofluorescence

TitleImproved double immunohistochemical staining method for cryostat and paraffin wax sections, combining alkaline phosphatase anti-alkaline phosphatase and indirect immunofluorescence
Authors
Issue Date1994
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
Citation
Journal Of Clinical Pathology, 1994, v. 47 n. 7, p. 597-600 How to Cite?
AbstractAims - To develop an immunohistochemical staining method for cryostat and paraffin wax sections so that two different antigens in the same section of tissues could be detected by combining immunoenzyme and immunofluorescence techniques. Methods - This double immunohistochemical staining method combines alkaline phosphatase-anti-alkaline phosphatase (APAAP) using New Fuchsin as a chromogen and indirect immunofluorescence. Results - APAAP staining for one antigen of this double immunohistochemical staining method was observed under bright field conditions alternating with immunofluorescence for another antigen under ultraviolet light. The double exposed photograph of both easily identified the two signals within the same cell. Conclusions - This double immunohistochemical staining method can overcome the disadvantages of any masking effect of the double immunoenzymatic methods and the background problems of double immunofluorescence method especially when applied to paraffin wax sections. It also permits good morphological identification of the doubly stained cells which may be of crucial importance in studies on pathology specimens.
Persistent Identifierhttp://hdl.handle.net/10722/43608
ISSN
2015 Impact Factor: 2.912
2015 SCImago Journal Rankings: 1.260
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTao, Qen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorLoke, SLen_HK
dc.contributor.authorChan, EYTen_HK
dc.contributor.authorHo, FCSen_HK
dc.date.accessioned2007-03-23T04:50:14Z-
dc.date.available2007-03-23T04:50:14Z-
dc.date.issued1994en_HK
dc.identifier.citationJournal Of Clinical Pathology, 1994, v. 47 n. 7, p. 597-600en_HK
dc.identifier.issn0021-9746en_HK
dc.identifier.urihttp://hdl.handle.net/10722/43608-
dc.description.abstractAims - To develop an immunohistochemical staining method for cryostat and paraffin wax sections so that two different antigens in the same section of tissues could be detected by combining immunoenzyme and immunofluorescence techniques. Methods - This double immunohistochemical staining method combines alkaline phosphatase-anti-alkaline phosphatase (APAAP) using New Fuchsin as a chromogen and indirect immunofluorescence. Results - APAAP staining for one antigen of this double immunohistochemical staining method was observed under bright field conditions alternating with immunofluorescence for another antigen under ultraviolet light. The double exposed photograph of both easily identified the two signals within the same cell. Conclusions - This double immunohistochemical staining method can overcome the disadvantages of any masking effect of the double immunoenzymatic methods and the background problems of double immunofluorescence method especially when applied to paraffin wax sections. It also permits good morphological identification of the doubly stained cells which may be of crucial importance in studies on pathology specimens.en_HK
dc.format.extent1225479 bytes-
dc.format.extent25088 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.en_HK
dc.subject.meshAlkaline phosphataseen_HK
dc.subject.meshImmunologyen_HK
dc.subject.meshAntigens - analysisen_HK
dc.subject.meshImmunoenzyme techniquesen_HK
dc.subject.meshImmunohistochemistry - methodsen_HK
dc.titleImproved double immunohistochemical staining method for cryostat and paraffin wax sections, combining alkaline phosphatase anti-alkaline phosphatase and indirect immunofluorescenceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=47&issue=7&spage=597&epage=600&date=1994&atitle=Improved+double+immunohistochemical+staining+method+for+cryostat+and+paraffin+wax+sections,+combining+alkaline+phosphatase+anti-alkaline+phosphatase+and+indirect+immunofluorescenceen_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid8089213-
dc.identifier.pmcidPMC502070-
dc.identifier.scopuseid_2-s2.0-0028648842en_HK
dc.identifier.hkuros5245-
dc.identifier.volume47en_HK
dc.identifier.issue7en_HK
dc.identifier.spage597en_HK
dc.identifier.epage600en_HK
dc.identifier.isiWOS:A1994NW73700006-
dc.publisher.placeUnited Kingdomen_HK

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