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Article: Cell-cycle-dependent localisation of Ulp1, a Schizosaccharomyces pombe Pmt3 (SUMO)-specific protease

TitleCell-cycle-dependent localisation of Ulp1, a Schizosaccharomyces pombe Pmt3 (SUMO)-specific protease
Authors
Issue Date2002
PublisherCompany of Biologists Ltd.
Citation
Journal of Cell Science, 2002, v. 115 n. pt 6, p. 1113-1122 How to Cite?
AbstractWe report here on the characterisation of Ulp1, a component of the SUMO modification process in S. pombe. Recombinant S. pombe Ulp1 has de-sumoylating activity; it is involved in the processing of Pmt3 (S. pombe SUMO) and can, to a limited extent, remove Pmt3 from modified targets in S. pombe cell extracts. ulp1 is not essential for cell viability, but cells lacking the gene display severe cell and nuclear abnormalities. ulp1-null (ulp1.d) cells are sensitive to ultraviolet radiation in a manner similar to rad31.d and hus5.62, which have mutations in one subunit of the activator and the conjugator for the ubiquitin-like protein SUMO respectively. However ulp1.d cells are less sensitive to ionising radiation and hydroxyurea (HU) than are rad31.d and hus5.62. ulp1-null cells are defective in processing precursor Pmt3 and display reduced levels of Pmt3 conjugates compared with wild-type cells. The slow growth phenotype of ulp1 null cells is not substantially rescued by over-expression of the mature form of Pmt3 (Pmt3-GG), suggesting that the de-conjugating activity of Ulp1 is required for normal cell cycle progression. During the S and G2 phases of the cell cycle the Ulp1 protein is localised to the nuclear periphery. However, during mitosis the pattern of staining alters, and during anaphase, Ulp1 is observed within the nucleus. Ulp1 localisation at the nuclear periphery is generally re-established by the time of septation (S phase).
Persistent Identifierhttp://hdl.handle.net/10722/43516
ISSN
2015 Impact Factor: 4.706
2015 SCImago Journal Rankings: 3.501
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTaylor, DLen_HK
dc.contributor.authorHo, JCYen_HK
dc.contributor.authorOliver, Aen_HK
dc.contributor.authorWatts, FZen_HK
dc.date.accessioned2007-03-23T04:47:42Z-
dc.date.available2007-03-23T04:47:42Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal of Cell Science, 2002, v. 115 n. pt 6, p. 1113-1122en_HK
dc.identifier.issn0021-9533en_HK
dc.identifier.urihttp://hdl.handle.net/10722/43516-
dc.description.abstractWe report here on the characterisation of Ulp1, a component of the SUMO modification process in S. pombe. Recombinant S. pombe Ulp1 has de-sumoylating activity; it is involved in the processing of Pmt3 (S. pombe SUMO) and can, to a limited extent, remove Pmt3 from modified targets in S. pombe cell extracts. ulp1 is not essential for cell viability, but cells lacking the gene display severe cell and nuclear abnormalities. ulp1-null (ulp1.d) cells are sensitive to ultraviolet radiation in a manner similar to rad31.d and hus5.62, which have mutations in one subunit of the activator and the conjugator for the ubiquitin-like protein SUMO respectively. However ulp1.d cells are less sensitive to ionising radiation and hydroxyurea (HU) than are rad31.d and hus5.62. ulp1-null cells are defective in processing precursor Pmt3 and display reduced levels of Pmt3 conjugates compared with wild-type cells. The slow growth phenotype of ulp1 null cells is not substantially rescued by over-expression of the mature form of Pmt3 (Pmt3-GG), suggesting that the de-conjugating activity of Ulp1 is required for normal cell cycle progression. During the S and G2 phases of the cell cycle the Ulp1 protein is localised to the nuclear periphery. However, during mitosis the pattern of staining alters, and during anaphase, Ulp1 is observed within the nucleus. Ulp1 localisation at the nuclear periphery is generally re-established by the time of septation (S phase).en_HK
dc.format.extent525575 bytes-
dc.format.extent25088 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherCompany of Biologists Ltd.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshCysteine endopeptidases - analysis - genetics - physiologyen_HK
dc.subject.meshRepressor proteins - analysis - genetics - metabolismen_HK
dc.subject.meshSchizosaccharomyces - enzymology - genetics - metabolism - radiation effectsen_HK
dc.subject.meshSchizosaccharomyces pombe proteinsen_HK
dc.subject.meshNucleic acid amplification techniquesen_HK
dc.titleCell-cycle-dependent localisation of Ulp1, a Schizosaccharomyces pombe Pmt3 (SUMO)-specific proteaseen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9533&volume=115&issue=pt 6&spage=1113&epage=1122&date=2002&atitle=Cell-cycle-dependent+localisation+of+Ulp1,+a+Schizosaccharomyces+pombe+Pmt3+(SUMO)-specific+proteaseen_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid11884512-
dc.identifier.scopuseid_2-s2.0-0037087504-
dc.identifier.hkuros89288-
dc.identifier.isiWOS:000174773500004-

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